James R. Krycer
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View article: Contractile function maintains cardiomyocyte differentiation and inhibits cell cycle activity
Contractile function maintains cardiomyocyte differentiation and inhibits cell cycle activity Open
Numerous endotherm species lose cardiac regenerative capacity shortly after birth, which is in contrast to many ectotherm species who regenerate throughout life. Whether the enhanced contractile function required for endothermy contributes…
View article: Maturation of human cardiac organoids enables complex disease modeling and drug discovery
Maturation of human cardiac organoids enables complex disease modeling and drug discovery Open
View article: Muscle-specific Ryanodine receptor 1 properties underlie limb-girdle muscular dystrophy 2B/R2 progression
Muscle-specific Ryanodine receptor 1 properties underlie limb-girdle muscular dystrophy 2B/R2 progression Open
View article: Tackling pressure fluctuations in ultra-HPLC to robustly resolve and analyze polar metabolites
Tackling pressure fluctuations in ultra-HPLC to robustly resolve and analyze polar metabolites Open
View article: Author Correction: Limited oxygen in standard cell culture alters metabolism and function of differentiated cells
Author Correction: Limited oxygen in standard cell culture alters metabolism and function of differentiated cells Open
View article: Limited oxygen in standard cell culture alters metabolism and function of differentiated cells
Limited oxygen in standard cell culture alters metabolism and function of differentiated cells Open
View article: A miniaturized culture platform for control of the metabolic environment
A miniaturized culture platform for control of the metabolic environment Open
The heart is a metabolic “omnivore” and adjusts its energy source depending on the circulating metabolites. Human cardiac organoids, a three-dimensional in vitro model of the heart wall, are a useful tool to study cardiac physiology and pa…
View article: Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance
Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance Open
The failure of metabolic tissues to appropriately respond to insulin (“insulin resistance”) is an early marker in the pathogenesis of type 2 diabetes. Protein phosphorylation is central to the adipocyte insulin response, but how adipocyte …
View article: Limited oxygen availability in standard cell culture alters metabolism and function in terminally-differentiated cells
Limited oxygen availability in standard cell culture alters metabolism and function in terminally-differentiated cells Open
SUMMARY Cell culture is generally considered to be hyperoxic. However, the importance of cellular oxygen consumption is often underappreciated, with rates of oxygen consumption often sufficient to cause hypoxia at cell monolayers. We initi…
View article: Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance
Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance Open
The failure of metabolic tissues to appropriately respond to insulin (“insulin resistance”) is an early marker in the pathogenesis of type 2 diabetes. Protein phosphorylation is central to the adipocyte insulin response, but how adipocyte …
View article: Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate
Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate Open
Trafficking regulator of GLUT4-1, TRARG1, positively regulates insulin-stimulated GLUT4 trafficking and insulin sensitivity. However, the mechanism(s) by which this occurs remain(s) unclear. Using biochemical and mass spectrometry analyses…
View article: A cell culture platform for quantifying metabolic substrate oxidation in bicarbonate-buffered medium
A cell culture platform for quantifying metabolic substrate oxidation in bicarbonate-buffered medium Open
View article: Dissecting the biology of mTORC1 beyond rapamycin
Dissecting the biology of mTORC1 beyond rapamycin Open
RapaLink1 enables differentiation between functions mediated by the closely related mTORC1 and mTORC2.
View article: Genome-wide analysis in <i>Drosophila</i> reveals diet-by-gene interactions and uncovers diet-responsive genes
Genome-wide analysis in <i>Drosophila</i> reveals diet-by-gene interactions and uncovers diet-responsive genes Open
Genetic and environmental factors play a major role in metabolic health. However, they do not act in isolation, as a change in an environmental factor such as diet may exert different effects based on an individual’s genotype. Here, we sou…
View article: Cannabichromene and Δ<sup>9</sup>-Tetrahydrocannabinolic Acid Identified as Lactate Dehydrogenase-A Inhibitors by <i>in Silico</i> and <i>in Vitro</i> Screening
Cannabichromene and Δ<sup>9</sup>-Tetrahydrocannabinolic Acid Identified as Lactate Dehydrogenase-A Inhibitors by <i>in Silico</i> and <i>in Vitro</i> Screening Open
Cannabis sativa contains >120 phytocannabinoids, but our understanding of these compounds is limited. Determining the molecular modes of action of the phytocannabinoids may assist in their therapeutic development. Ligand-based virtu…
View article: Metabolic buffer analysis reveals the simultaneous, independent control of ATP and adenylate energy ratios
Metabolic buffer analysis reveals the simultaneous, independent control of ATP and adenylate energy ratios Open
Determining the underlying principles behind biological regulation is important for understanding the principles of life, treating complex diseases and creating de novo synthetic biology. Buffering—the use of reservoirs of molecules to mai…
View article: Gut-derived acetate promotes B10 cells with antiinflammatory effects
Gut-derived acetate promotes B10 cells with antiinflammatory effects Open
Autoimmune diseases are characterized by a breakdown of immune tolerance partly due to environmental factors. The short-chain fatty acid acetate, derived mostly from gut microbial fermentation of dietary fiber, promotes antiinflammatory Tr…
View article: Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate
Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate Open
Trafficking regulator of GLUT4-1, TRARG1, positively regulates insulin-stimulated GLUT4 trafficking and insulin sensitivity. However, the mechanism(s) by which this occurs remain(s) unclear. Using biochemical and mass spectrometry analyses…
View article: Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin
Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin Open
[This corrects the article DOI: 10.1016/j.isci.2020.100855.].
View article: Kinetic Trans-omic Analysis Reveals Key Regulatory Mechanisms for Insulin-Regulated Glucose Metabolism in Adipocytes
Kinetic Trans-omic Analysis Reveals Key Regulatory Mechanisms for Insulin-Regulated Glucose Metabolism in Adipocytes Open
Insulin regulates glucose metabolism through thousands of regulatory mechanisms; however, which regulatory mechanisms are keys to control glucose metabolism remains unknown. Here, we performed kinetic trans-omic analysis by integrating iso…
View article: Insulin signaling requires glucose to promote lipid anabolism in adipocytes
Insulin signaling requires glucose to promote lipid anabolism in adipocytes Open
Adipose tissue is essential for metabolic homeostasis, balancing lipid storage and mobilization based on nutritional status. This is coordinated by insulin, which triggers kinase signaling cascades to modulate numerous metabolic proteins, …
View article: Temporal ordering of omics and multiomic events inferred from time-series data
Temporal ordering of omics and multiomic events inferred from time-series data Open
View article: Temporal ordering of omics and multiomic events inferred from time series data
Temporal ordering of omics and multiomic events inferred from time series data Open
Temporal changes in omics events can now be routinely measured, however current analysis methods are often inadequate, especially for multiomics experiments. We report a novel analysis method that can infer event ordering at better tempora…
View article: Is Mitochondrial Dysfunction a Common Root of Noncommunicable Chronic Diseases?
Is Mitochondrial Dysfunction a Common Root of Noncommunicable Chronic Diseases? Open
Mitochondrial damage is implicated as a major contributing factor for a number of noncommunicable chronic diseases such as cardiovascular diseases, cancer, obesity, and insulin resistance/type 2 diabetes. Here, we discuss the role of mitoc…
View article: Metabolic Buffer Analysis Reveals the Simultaneous, Independent Control of ATP and Adenylate Energy Ratios
Metabolic Buffer Analysis Reveals the Simultaneous, Independent Control of ATP and Adenylate Energy Ratios Open
Determining the underlying principles behind biological regulation is important for understanding the principles of life, treating complex diseases, and creating de novo synthetic biology. Buffering - the use of reservoirs of molecules to …
View article: Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin
Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin Open
View article: Mitochondrial oxidants, but not respiration, are sensitive to glucose in adipocytes
Mitochondrial oxidants, but not respiration, are sensitive to glucose in adipocytes Open
Insulin action in adipose tissue is crucial for whole-body glucose homeostasis, with insulin resistance being a major risk factor for metabolic diseases such as type 2 diabetes. Recent studies have proposed mitochondrial oxidants as a unif…
View article: Lactate production is a prioritized feature of adipocyte metabolism
Lactate production is a prioritized feature of adipocyte metabolism Open
Adipose tissue is essential for whole-body glucose homeostasis, with a primary role in lipid storage. It has been previously observed that lactate production is also an important metabolic feature of adipocytes, but its relationship to adi…
View article: Serine 474 phosphorylation is essential for maximal Akt2 kinase activity in adipocytes
Serine 474 phosphorylation is essential for maximal Akt2 kinase activity in adipocytes Open
View article: A Modified Gas-Trapping Method for High-Throughput Metabolic Experiments in <i>Drosophila Melanogaster</i>
A Modified Gas-Trapping Method for High-Throughput Metabolic Experiments in <i>Drosophila Melanogaster</i> Open
Metabolism is often studied in animal models, with the Drosophila melanogaster fruit fly model offering ease of genetic manipulation and high-throughput studies. Fly metabolism is typically studied using end-point assays that are si…