Anthony Maxwell
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View article: DNA Topoisomerase VI: Structure, Function and Mechanism
DNA Topoisomerase VI: Structure, Function and Mechanism Open
DNA topoisomerase VI (topo VI) is a type IIB topoisomerase that was originally found in archaea but later found in plants and some other eukaryotes and certain bacteria; claims that it is present in plasmodial parasites have yet to be subs…
View article: Developing a Gram-Negative Selective Peptide–Drug Conjugate
Developing a Gram-Negative Selective Peptide–Drug Conjugate Open
Resistance to fluoroquinolone antibiotics has serious implications for healthcare; here, we conjugate the widely used fluoroquinolone ciprofloxacin to a proline-rich antimicrobial peptide (PrAMP) oncocin to improve oncocin's potency in cip…
View article: A conserved region T-cell vaccine for Sarbecoviruses
A conserved region T-cell vaccine for Sarbecoviruses Open
The rapid development of vaccines was a critical part of the global response to the COVID-19 pandemic. SARS-CoV-2 (a Sarbecovirus and member of the Betacoronavirus genus responsible for the pandemic) virus was first detected in Wuhan, Chin…
View article: Undergraduate Students’ Experiences of a Community-Engaged Learning Course: A Mixed-Methods Study
Undergraduate Students’ Experiences of a Community-Engaged Learning Course: A Mixed-Methods Study Open
Background Undergraduate student engagement increases the quality of education. Community-engaged learning (CEL) courses are one way to promote engagement and involve students collaborating with community partners to achieve a common goal …
View article: DNA Topoisomerase Vi: Biology and Mechanism
DNA Topoisomerase Vi: Biology and Mechanism Open
View article: Insights into antibiotic resistance promoted by quinolone exposure
Insights into antibiotic resistance promoted by quinolone exposure Open
Quinolone-induced antibiotic resistance (QIAR) refers to the phenomenon by which bacteria exposed to sublethal levels of quinolones acquire resistance to non-quinolone antibiotics. We have explored this in Escherichia coli MG1655 using a v…
View article: Structural Aspects of <i>Mycobacterium tuberculosis</i> DNA Gyrase Targeted by Novel Bacterial Topoisomerase Inhibitors
Structural Aspects of <i>Mycobacterium tuberculosis</i> DNA Gyrase Targeted by Novel Bacterial Topoisomerase Inhibitors Open
In this Letter, we present a small series of novel bacterial topoisomerase inhibitors (NTBIs) that exhibit both potent inhibition of Mycobacterium tuberculosis DNA gyrase and potent antimycobacterial activity. The disclosed crystal …
View article: Highly sensitive mapping of <i>in vitro</i> type II topoisomerase DNA cleavage sites with SHAN-seq
Highly sensitive mapping of <i>in vitro</i> type II topoisomerase DNA cleavage sites with SHAN-seq Open
Type II topoisomerases (topos) are a ubiquitous and essential class of enzymes that form transient enzyme-bound double-stranded breaks on DNA called cleavage complexes. The location and frequency of these cleavage complexes on DNA is impor…
View article: Phylogenetic distribution of DNA topoisomerase VI and its distinction from SPO11
Phylogenetic distribution of DNA topoisomerase VI and its distinction from SPO11 Open
DNA topoisomerases (topos) are major targets for antimicrobial and chemotherapeutic drugs due to their fundamental roles in regulating DNA topology. Type II topos are essential for chromosome segregation and relaxing positive DNA supercoil…
View article: Exploring the interaction of N-(benzothiazol-2-yl)pyrrolamide DNA gyrase inhibitors with the GyrB ATP-binding site lipophilic floor: A medicinal chemistry and QTAIM study
Exploring the interaction of N-(benzothiazol-2-yl)pyrrolamide DNA gyrase inhibitors with the GyrB ATP-binding site lipophilic floor: A medicinal chemistry and QTAIM study Open
View article: Author response: Rapid, DNA-induced interface swapping by DNA gyrase
Author response: Rapid, DNA-induced interface swapping by DNA gyrase Open
View article: Rapid, DNA-induced interface swapping by DNA gyrase
Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. DNA gyrase can loop DNA around the C-terminal domains (CTDs) of GyrA…
View article: Highly sensitive mapping of<i>in vitro</i>type II topoisomerase DNA cleavage sites with SHAN-seq
Highly sensitive mapping of<i>in vitro</i>type II topoisomerase DNA cleavage sites with SHAN-seq Open
Type II topoisomerases (topos) are a ubiquitous and essential class of enzymes that form transient enzyme-bound double-stranded breaks on DNA called cleavage complexes. The location and frequency of these cleavage complexes on DNA is impor…
View article: Transplanting human infant gut microbiome species into Galleria mellonella
Transplanting human infant gut microbiome species into Galleria mellonella Open
Objective Study of the human infant gut microbiome requires the use of surrogate mammalian species such as mice. We sought to investigate the usefulness of the greater wax moth larva, Galleria mellonella , as an alternative. Results We hav…
View article: Rapid, DNA-induced interface swapping by DNA gyrase
Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist…
View article: Author Response: Rapid, DNA-induced interface swapping by DNA gyrase
Author Response: Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist…
View article: Transplanting human infant gut microbiome species into Galleria mellonella
Transplanting human infant gut microbiome species into Galleria mellonella Open
ObjectiveStudy of the human infant gut microbiome requires the use of surrogate mammalian species such as mice. We sought to investigate the usefulness of the greater wax moth larva, Galleria mellonella, as an alternative. Results We have …
View article: Structural Aspects of Mycobacterium Tuberculosis DNA Gyrase Inhibition by the Novel Bacterial Topoisomerase
Structural Aspects of Mycobacterium Tuberculosis DNA Gyrase Inhibition by the Novel Bacterial Topoisomerase Open
View article: Exploring the 5-Substituted 2-Aminobenzothiazole-Based DNA Gyrase B Inhibitors Active against ESKAPE Pathogens
Exploring the 5-Substituted 2-Aminobenzothiazole-Based DNA Gyrase B Inhibitors Active against ESKAPE Pathogens Open
We present a new series of 2-aminobenzothiazole-based DNA gyrase B inhibitors with promising activity against ESKAPE bacterial pathogens. Based on the binding information extracted from the cocrystal structure of DNA gyrase B inhibitor …
View article: Rapid, DNA-induced subunit exchange by DNA gyrase
Rapid, DNA-induced subunit exchange by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist…
View article: Rapid, DNA-induced interface swapping by DNA gyrase
Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. DNA gyrase can loop DNA around the C-terminal domains (CTDs) of GyrA…
View article: New Dual Inhibitors of Bacterial Topoisomerases with Broad-Spectrum Antibacterial Activity and In Vivo Efficacy against Vancomycin-Intermediate <i>Staphylococcus aureus</i>
New Dual Inhibitors of Bacterial Topoisomerases with Broad-Spectrum Antibacterial Activity and In Vivo Efficacy against Vancomycin-Intermediate <i>Staphylococcus aureus</i> Open
A new series of dual low nanomolar benzothiazole inhibitors of bacterial DNA gyrase and topoisomerase IV were developed. The resulting compounds show excellent broad-spectrum antibacterial activities against Gram-positive Enterococcus f…
View article: Antimicrobial resistance: a Biochemical Society position statement
Antimicrobial resistance: a Biochemical Society position statement Open
Part of the Biochemical Society’s mission is to champion the molecular biosciences and their importance in addressing societal grand challenges. To achieve this, the Society undertakes policy work, aiming to channel the views of its member…
View article: Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold-Based DNA Gyrase Inhibitors with Potent Activity against <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i>
Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold-Based DNA Gyrase Inhibitors with Potent Activity against <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> Open
We have developed compounds with a promising activity against Acinetobacter baumannii and Pseudomonas aeruginosa, which are both on the WHO priority list of antibiotic-resistant bacteria. Starting from DNA gyrase inhibitor
View article: Rapid, DNA-induced interface swapping by DNA gyrase
Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist…
View article: A simplified and easy‐to‐use <scp>HIP HOP</scp> assay provides insights into chalcone antifungal mechanisms of action
A simplified and easy‐to‐use <span>HIP HOP</span> assay provides insights into chalcone antifungal mechanisms of action Open
Elucidating the mechanism of action of an antifungal or cytotoxic compound is a time‐consuming process. Yeast chemogenomic profiling provides a compelling solution to the problem but is experimentally complex. Here, we demonstrate the use …
View article: Topoisomerase VI is a chirally-selective, preferential DNA decatenase
Topoisomerase VI is a chirally-selective, preferential DNA decatenase Open
DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence …
View article: <i>De novo</i> design of type II topoisomerase inhibitors as potential antimicrobial agents targeting a novel binding region
<i>De novo</i> design of type II topoisomerase inhibitors as potential antimicrobial agents targeting a novel binding region Open
A computational design, make and test strategy was used to identify antibacterial inhibitors of bacterial DNA gyrase and topoisomerase IV, proposed to bind at a novel allosteric site.
View article: Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella
Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella Open
Four bacterial strains were isolated from two different colony sources of the wax moth Galleria mellonella . They were characterized by a polyphasic approach including 16S rRNA gene sequence analysis, core-genome analysis, average nucleoti…
View article: De Novo Design of Type II Topoisomerase Inhibitors as Potential Antimicrobial Agents Targeting a Novel Binding Region
De Novo Design of Type II Topoisomerase Inhibitors as Potential Antimicrobial Agents Targeting a Novel Binding Region Open
By 2050 it is predicted that antimicrobial resistance will be responsible for 10 million global deaths annually, costing the world economy $100 trillion. Clearly, strategies to address this problem are required as bacterial evolution is re…