James W. Schumm
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Buccal DNA Samples for DNA Typing: New Collection and Processing Methods Open
With the recent expansion of DNA database laws in many states, there is a critical need for the rapid and simple collection of DNA samples and streamlined processing for downstream applications. The Buccal DNA Collector was developed to ad…
Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis Open
Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enabl…
Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis Open
Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing. To reduce the drawback of preparing gels or using exp…
IPO volume 27 issue 3 Cover and Front matter Open
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Multiplex Sets for the Amplification of Polymorphic Short Tandem Repeat Loci — Silver Stain and Fluorescence Detection Open
Multiplex PCR amplification systems were developed using well-characterized, polymorphic short tandem repeat (STR) loci. Eight loci utilized in the multiplex amplifications included HUMCSF1PO, HUMTPOX, HUMTH01, HUMVWFA31, HUMF13A01, HUMFES…
General Approach to Analysis of Polymorphic Short Tandem Repeat Loci Open
Polymerase chain reaction amplification products of 22 known polymorphic short tandem repeat (STR) loci were subjected to denaturing polyacrylamide gel electrophoresis and detected using a silver staining method. Loci that amplified effici…
Genetic linkage map of human chromosome 7 with 63 DNA markers. Open
High-density genetic maps of individual human chromosomes will permit accurate localization of disease-associated genes and provide signposts that will be useful in the construction of physical maps. We have constructed a genetic map of ch…