Jameson R. Bothe
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View article: Polymorphs in a Pandemic: Structure Elucidation of Polymorphs of Molnupiravir
Polymorphs in a Pandemic: Structure Elucidation of Polymorphs of Molnupiravir Open
Molnupiravir, a small molecule active pharmaceutical ingredient developed through a partnership between Merck and Ridgeback Biotherapeutics, was shown to be efficacious for the treatment of patients with COVID-19. Early in development it w…
View article: Leveraging SAXS for Biologics Formulation Development in the Pharmaceutical Industry
Leveraging SAXS for Biologics Formulation Development in the Pharmaceutical Industry Open
Formulation development of biologics is key for ensuring the safety and efficacy of biologics drug products such as monoclonal antibodies (mAbs) due to their complex degradation and aggregation pathways. Work has been completed by collabor…
View article: A Precipitation-Based Process to Generate a Solid Formulation of a Therapeutic Monoclonal Antibody: An Alternative to Lyophilization
A Precipitation-Based Process to Generate a Solid Formulation of a Therapeutic Monoclonal Antibody: An Alternative to Lyophilization Open
Lyophilization, or freeze-drying, is the default technique for the manufacture of solid-state formulations of therapeutic proteins. This established method offers several advantages, including improved product stability by minimizing chemi…
View article: Learning relationships between chemical and physical stability for drug development
Learning relationships between chemical and physical stability for drug development Open
Chemical and physical stabilities are two key features considered in pharmaceutical development. Chemical stability is typically reported as a combination of potency and degradation product. For peptide products, it is common to measure ph…
View article: The metal chaperone Atox1 regulates the activity of the human copper transporter ATP7B by modulating domain dynamics
The metal chaperone Atox1 regulates the activity of the human copper transporter ATP7B by modulating domain dynamics Open
The human transporter ATP7B delivers copper to the biosynthetic pathways and maintains copper homeostasis in the liver. Mutations in ATP7B cause the potentially fatal hepatoneurological disorder Wilson disease. The activity and intracellul…
View article: Development and Application of a High Throughput Protein Unfolding Kinetic Assay
Development and Application of a High Throughput Protein Unfolding Kinetic Assay Open
The kinetics of folding and unfolding underlie protein stability and quantification of these rates provides important insights into the folding process. Here, we present a simple high throughput protein unfolding kinetic assay using a plat…
View article: The Complex Energy Landscape of the Protein IscU
The Complex Energy Landscape of the Protein IscU Open
IscU, the scaffold protein for iron-sulfur (Fe-S) cluster biosynthesis in Escherichia coli, traverses a complex energy landscape during Fe-S cluster synthesis and transfer. Our previous studies showed that IscU populates two interconvertin…