Jamie W. Joseph
YOU?
Author Swipe
View article: Effect of Ankle Foot Orthoses on Gait Parameter among Children with Spastic Diplegic Cerebral Palsy
Effect of Ankle Foot Orthoses on Gait Parameter among Children with Spastic Diplegic Cerebral Palsy Open
Objective: This study evaluated the effects of solid ankle-foot orthosis (SAFO) and ground reaction ankle-foot orthosis (GRAFO) on gait parameters (kinematic and temporospatial) in children with diplegic spastic cerebral palsy (CP).Materia…
View article: Enabling Open Machine Learning of Deoxyribonucleic Acid-Encoded Library Selections to Accelerate the Discovery of Small Molecule Protein Binders
Enabling Open Machine Learning of Deoxyribonucleic Acid-Encoded Library Selections to Accelerate the Discovery of Small Molecule Protein Binders Open
Machine learning (ML) is increasingly used in DNA-encoded library (DEL) screening for ligand discovery, but its success depends on access to suitable data sets, which are often proprietary and costly. To overcome this, we present the first…
View article: Evaluating the impact of lipids in isolated islet research
Evaluating the impact of lipids in isolated islet research Open
Pancreatic β-cells secrete insulin in response to nutrient stimulation. Glucose, amino acids and free fatty acids (FFA) have all been shown to stimulate insulin release in vivo . In vitro , isolated islet studies have also demonstrated sim…
View article: ARC4CFD: Learning how to leverage High-Performance Computing with Computational Fluid Dynamics
ARC4CFD: Learning how to leverage High-Performance Computing with Computational Fluid Dynamics Open
View article: The effects of free fatty acid-free bovine serum albumin and palmitate on pancreatic β-cell function
The effects of free fatty acid-free bovine serum albumin and palmitate on pancreatic β-cell function Open
Pancreatic β-cells release insulin in response to fluctuations in plasma glucose, amino acids, and free fatty acids (FFA). Clonal cell lines and isolated islets serve as essential early models for studying the impact of nutrients and evalu…
View article: Enabling Open Machine Learning of DNA Encoded Library Selections to Accelerate the Discovery of Small Molecule Protein Binders
Enabling Open Machine Learning of DNA Encoded Library Selections to Accelerate the Discovery of Small Molecule Protein Binders Open
Recent advances in DNA-encoded library (DEL) screening have created bioactivity datasets containing billions of molecules, unlocking new opportunities for machine learning (ML) in drug discovery. However, most ultra-large DEL libraries are…
View article: Renal tubule-specific Atgl deletion links kidney lipid metabolism to glucagon-like peptide 1 and insulin secretion independent of renal inflammation or lipotoxicity
Renal tubule-specific Atgl deletion links kidney lipid metabolism to glucagon-like peptide 1 and insulin secretion independent of renal inflammation or lipotoxicity Open
TAG over-storage alone is insufficient to cause renal tubule lipotoxicity. This work is the first to show that endogenously derived LPA modulates GLP-1 levels in vivo, demonstrating a new mechanism of kidney-gut-pancreas crosstalk to regul…
View article: Kinetic modelling of <i>β</i> ‐cell metabolism reveals control points in the insulin‐regulating pyruvate cycling pathways
Kinetic modelling of <i>β</i> ‐cell metabolism reveals control points in the insulin‐regulating pyruvate cycling pathways Open
Insulin, a key hormone in the regulation of glucose homoeostasis, is secreted by pancreatic β ‐cells in response to elevated glucose levels. Insulin is released in a biphasic manner in response to glucose metabolism in β ‐cells. The first …
View article: Supplementary Tables S1-S3 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Tables S1-S3 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Supplementary Tables S1-S3. Summary of EC50 values for avocatin B in a panel of AML cell lines (S1); AML patient sample details used for annexin/PI (S2); AML patient sample details used for colony formation assays (S3).
View article: Supplementary Figures S1-S9 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Figures S1-S9 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Supplementary Figures S1-S9. Avocatin B is the most active avocado lipid analogue (S1); Kinetics of avocatin B-induced death (S2); Cell cycle analysis of avocatin B treated TEX cells (S3); Avocatin B increases DCFH-DA and DHE (S4); Avocati…
View article: Supplementary Figure Legends from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Figure Legends from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Legend for Supplementary Figures S1-S9.
View article: Supplementary Methods and References from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Methods and References from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Supplementary Methods and References. Description of additional methods and procedures used in the study. Also includes Supplementary References.
View article: Data from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Data from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Treatment regimens for acute myeloid leukemia (AML) continue to offer weak clinical outcomes. Through a high-throughput cell-based screen, we identified avocatin B, a lipid derived from avocado fruit, as a novel compound with cytotoxic act…
View article: Supplementary Figures S1-S9 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Figures S1-S9 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Supplementary Figures S1-S9. Avocatin B is the most active avocado lipid analogue (S1); Kinetics of avocatin B-induced death (S2); Cell cycle analysis of avocatin B treated TEX cells (S3); Avocatin B increases DCFH-DA and DHE (S4); Avocati…
View article: Supplementary Methods and References from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Methods and References from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Supplementary Methods and References. Description of additional methods and procedures used in the study. Also includes Supplementary References.
View article: Data from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Data from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Treatment regimens for acute myeloid leukemia (AML) continue to offer weak clinical outcomes. Through a high-throughput cell-based screen, we identified avocatin B, a lipid derived from avocado fruit, as a novel compound with cytotoxic act…
View article: Supplementary Tables S1-S3 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Tables S1-S3 from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Supplementary Tables S1-S3. Summary of EC50 values for avocatin B in a panel of AML cell lines (S1); AML patient sample details used for annexin/PI (S2); AML patient sample details used for colony formation assays (S3).
View article: Supplementary Figure Legends from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Supplementary Figure Legends from Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Legend for Supplementary Figures S1-S9.
View article: Iatrogenic-induced Peripheral Gangrene: From Multiple Limb Amputations to Prosthetic Restoration
Iatrogenic-induced Peripheral Gangrene: From Multiple Limb Amputations to Prosthetic Restoration Open
Objectives: Multiple limb amputations are an uncommon complication from inotrope-induced peripheral gangrene. Case Presentation: A 20-year-old lady with valvular heart disease had septic shock secondary to infective endocarditis and requir…
View article: Prevention of Lipotoxicity in Pancreatic Islets with Gammahydroxybutyrate
Prevention of Lipotoxicity in Pancreatic Islets with Gammahydroxybutyrate Open
Oxidative stress caused by the exposure of pancreatic ß-cells to high levels of fatty acids impairs insulin secretion. This lipotoxicity is thought to play an important role in ß-cell failure in type 2 diabetes and can be prevented by anti…
View article: Nicotinamide Mononucleotide Prevents Free Fatty Acid-Induced Reduction in Glucose Tolerance by Decreasing Insulin Clearance
Nicotinamide Mononucleotide Prevents Free Fatty Acid-Induced Reduction in Glucose Tolerance by Decreasing Insulin Clearance Open
The NAD-dependent deacetylase SIRT1 improves β cell function. Accordingly, nicotinamide mononucleotide (NMN), the product of the rate-limiting step in NAD synthesis, prevents β cell dysfunction and glucose intolerance in mice fed a high-fa…
View article: Isoform-specific Roles of Prolyl Hydroxylases in the Regulation of Pancreatic β-Cell Function
Isoform-specific Roles of Prolyl Hydroxylases in the Regulation of Pancreatic β-Cell Function Open
Pancreatic β-cells can secrete insulin via 2 pathways characterized as KATP channel -dependent and -independent. The KATP channel–independent pathway is characterized by a rise in several potential metabolic signaling molecules, including …
View article: Kinetic modelling of β-cell metabolism reveals control points in the insulin-regulating pyruvate cycling pathways
Kinetic modelling of β-cell metabolism reveals control points in the insulin-regulating pyruvate cycling pathways Open
Insulin, a key hormone in the regulation of glucose homeostasis, is secreted by pancreatic β-cells in response to elevated glucose levels. Insulin is released in a biphasic manner in response to glucose metabolism in β-cells. The first pha…
View article: Prolyl-4-hydroxylase 3 maintains β cell glucose metabolism during fatty acid excess in mice
Prolyl-4-hydroxylase 3 maintains β cell glucose metabolism during fatty acid excess in mice Open
The α-ketoglutarate-dependent dioxygenase, prolyl-4-hydroxylase 3 (PHD3), is an HIF target that uses molecular oxygen to hydroxylate peptidyl prolyl residues. Although PHD3 has been reported to influence cancer cell metabolism and liver in…
View article: The role of α-ketoglutarate and the hypoxia sensing pathway in the regulation of pancreatic β-cell function
The role of α-ketoglutarate and the hypoxia sensing pathway in the regulation of pancreatic β-cell function Open
Anaplerosis and the associated mitochondrial metabolite transporters generate unique cytosolic metabolic signaling molecules that can regulate insulin release from pancreatic β-cells. It has been shown that mitochondrial metabolites, trans…
View article: The Loss of ARNT/HIF1β in Male Pancreatic β-Cells Is Protective Against High-Fat Diet–Induced Diabetes
The Loss of ARNT/HIF1β in Male Pancreatic β-Cells Is Protective Against High-Fat Diet–Induced Diabetes Open
The transcription factor aryl hydrocarbon receptor nuclear translocator (ARNT)/hypoxia-inducible factor (HIF)-1β (ARNT/HIF1β) plays a key role in maintaining β-cell function and has been shown to be one of the most downregulated transcript…
View article: Role of prolyl hydroxylase domain proteins in the regulation of insulin secretion
Role of prolyl hydroxylase domain proteins in the regulation of insulin secretion Open
Type 2 diabetes is associated with impaired nutrient-regulated anaplerosis and insulin secretion in pancreatic β-cells. One key anaplerotic substrate that may be involved in regulating insulin release is α-ketoglutarate (αKG). Since prolyl…
View article: Deletion of ARNT/HIF1β in pancreatic beta cells does not impair glucose homeostasis in mice, but is associated with defective glucose sensing ex vivo
Deletion of ARNT/HIF1β in pancreatic beta cells does not impair glucose homeostasis in mice, but is associated with defective glucose sensing ex vivo Open
View article: Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death
Targeting Mitochondria with Avocatin B Induces Selective Leukemia Cell Death Open
Treatment regimens for acute myeloid leukemia (AML) continue to offer weak clinical outcomes. Through a high-throughput cell-based screen, we identified avocatin B, a lipid derived from avocado fruit, as a novel compound with cytotoxic act…
View article: Effect of N-acetyl-l-cysteine on insulin resistance caused by prolonged free fatty acid elevation
Effect of N-acetyl-l-cysteine on insulin resistance caused by prolonged free fatty acid elevation Open
Circulating free fatty acids (FFAs) are elevated in obesity and cause insulin resistance. The objective of the current study was to determine whether the antioxidant N -acetyl- l -cysteine (NAC) prevented hepatic and peripheral insulin res…