Jan H. Forth
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View article: Full genome sequence analysis of African swine fever virus isolates from Cameroon
Full genome sequence analysis of African swine fever virus isolates from Cameroon Open
African swine fever (ASF) is a devastating disease of domestic pigs that has spread across the globe since its introduction into Georgia in 2007. The etiological agent is a large double-stranded DNA virus with a genome of 170 to 180 kb in …
View article: Full genome sequence analysis of African swine fever virus isolates from Cameroon
Full genome sequence analysis of African swine fever virus isolates from Cameroon Open
African swine fever is a devastating disease of domestic pigs that has spread across the globe since its introduction into Georgia in 2007. The etiological agent is a large double-stranded DNA virus with a genome of 170 to 180 kb in length…
View article: Characterization of a Novel African Swine Fever Virus p72 Genotype II from Nigeria
Characterization of a Novel African Swine Fever Virus p72 Genotype II from Nigeria Open
African swine fever (ASF) is a high-consequence transboundary hemorrhagic fever of swine. It continues to spread across the globe causing socio-economic issues and threatening food security and biodiversity. In 2020, Nigeria reported a maj…
View article: African swine fever virus – variants on the rise
African swine fever virus – variants on the rise Open
African swine fever virus (ASFV), a large and complex DNA-virus circulating between soft ticks and indigenous suids in sub-Saharan Africa, has made its way into swine populations from Europe to Asia. This virus, causing a severe haemorrhag…
View article: African swine fever virus – variants on the rise
African swine fever virus – variants on the rise Open
African swine fever virus (ASFV), a large and complex DNA-virus circulating between soft ticks and indigenous suids in sub-Saharan Africa, has made its way into swine populations from Europe to Asia. This virus, causing a severe haemorrhag…
View article: Taking a Promising Vaccine Candidate Further: Efficacy of ASFV-G-ΔMGF after Intramuscular Vaccination of Domestic Pigs and Oral Vaccination of Wild Boar
Taking a Promising Vaccine Candidate Further: Efficacy of ASFV-G-ΔMGF after Intramuscular Vaccination of Domestic Pigs and Oral Vaccination of Wild Boar Open
African swine fever (ASF) is a pandemic threat to the global pig industry and wild suids. A safe and efficacious vaccine could monumentally assist in disease eradication. In the past years, promising live attenuated vaccine candidates emer…
View article: Taking a Promising Vaccine Candidate Further: Efficacy of ASFV-G-ΔMGF after Intramuscular Vaccination of Domestic Pigs and Oral Vaccination of Wild Boar
Taking a Promising Vaccine Candidate Further: Efficacy of ASFV-G-ΔMGF after Intramuscular Vaccination of Domestic Pigs and Oral Vaccination of Wild Boar Open
African swine fever (ASF) is a pandemic threat to the global pig industry and wild suids. A safe and efficacious vaccine could monumentally assist in disease eradication. In the past years, promising live attenuated vaccine candidates emer…
View article: New Cell Lines Derived from European Tick Species
New Cell Lines Derived from European Tick Species Open
Tick cell lines are important tools for research on ticks and the pathogens they transmit. Here, we report the establishment of ten new cell lines from European ticks of the genera Argas, Dermacentor, Hyalomma, Ixodes and Rhipicephalus ori…
View article: African swine fever virus – variants on the rise
African swine fever virus – variants on the rise Open
African swine fever virus (ASFV), a large and complex DNA-virus circulating between soft ticks and indigenous suids in sub-Saharan Africa, has made its way into swine populations from Europe to Asia. This virus, causing a severe haemorrhag…
View article: African Swine Fever in Wild Boar in Europe—A Review
African Swine Fever in Wild Boar in Europe—A Review Open
The introduction of genotype II African swine fever (ASF) virus, presumably from Africa into Georgia in 2007, and its continuous spread through Europe and Asia as a panzootic disease of suids, continues to have a huge socio-economic impact…
View article: Correction to: A comparison of the cuticular properties of argasid and ixodid ticks: Ornithodoros moubata (Argasidae) vs. Amblyomma hebraeum and Ixodes pacificus (Ixodidae)
Correction to: A comparison of the cuticular properties of argasid and ixodid ticks: Ornithodoros moubata (Argasidae) vs. Amblyomma hebraeum and Ixodes pacificus (Ixodidae) Open
View article: Whole-Genome Sequence of an African Swine Fever Virus Isolate from the Czech Republic
Whole-Genome Sequence of an African Swine Fever Virus Isolate from the Czech Republic Open
Between June 2017 and April 2018, an outbreak of African swine fever (ASF) affected wild boar in the southeast of the Czech Republic. Here, we present the whole-genome sequence of the causative ASF virus. It belongs to genotype II and show…
View article: Joining the club: First detection of African swine fever in wild boar in Germany
Joining the club: First detection of African swine fever in wild boar in Germany Open
African swine fever (ASF) has spread across many countries in Europe since the introduction into Georgia in 2007. We report here on the first cases of ASF in wild boar detected in Germany close to the border with Poland. In addition to the…
View article: Joining the club: First detection of African swine fever in wild boar in Germany
Joining the club: First detection of African swine fever in wild boar in Germany Open
African swine fever (ASF) has spread across many countries in Europe since the introduction into Georgia in 2007. We report here on the first cases of ASF in wild boar detected in Germany close to the border with Poland. In addition to the…
View article: Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
View article: African swine fever whole-genome sequencing—Quantity wanted but quality needed
African swine fever whole-genome sequencing—Quantity wanted but quality needed Open
The pandemic spread of African swine fever virus (ASFV) genotype II (GTII) has led to a global crisis. Since the circulating strains are almost identical, time and money have been mis-invested in whole-genome sequencing the last years. New…
View article: African swine fever virus-like integrated elements in a soft tick genome – an ancient virus vector arms race?
African swine fever virus-like integrated elements in a soft tick genome – an ancient virus vector arms race? Open
Background African swine fever virus (ASFV) is the only known DNA-arbovirus and a most devastating suid pathogen that, originating from a sylvatic cycle in Africa, has spread to eastern Europe and recently reached western Europe and Asia, …
View article: Additional file 2 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 2 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 2: Table S2. ASFV-like transcripts detected in data from six Ornithodoros moubata cell lines and O.moubata ticks by mRNA-sequencing and data analysis from published sequences ASFV-like sequences from tick cell libraries lib…
View article: Additional file 6 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 6 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 6: Table S5. Results of tick and tick cell line screening for ASFLI-elements by qPCR. DNA from single ticks and every tick cell line (n=1) was extracted and analysed by qPCR for six ASFV-like genes as described in the ‘Mate…
View article: Additional file 15 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 15 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 15: Table S9. Results of small RNA sequencing and mapping against ASFV and ASFLI-elements. Small RNA was sequenced from Ornithodoros porcinus and Ornithodoros moubata nymphal stage ticks. After deduplication using BBMap, 22…
View article: Additional file 1 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 1 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 1: Table S1. Overview of NGS experiments conducted in this study and data obtained from the literature. Ornithodoros moubata cell line names have been abbreviated by removal of “/CTVM” and Ornithodoros porcinus and Ornithod…
View article: Additional file 16 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 16 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 16: Table S10. O. moubata piRNA ping-pong signature analysed by PingPongPro v1.0 with default parameters and -b option (creates additional browser track files, which are suitable for display in common genome browsers). The …
View article: Additional file 19 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 19 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 19: Table S12.Primer and probe sequences used in this study.
View article: Additional file 7 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 7 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 7: Table S6. BLASTn results of SPAdes-assembled contigs from Ornithodoro porcinus France and Kenya17 containing ASFLI-elements. Shown are hits with the lowest e-value for ASFV-genes as obtained by Blastn against the entire …
View article: Additional file 10 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 10 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 10: Table S8. qRT-PCR results of Ornithodoros ticks experimentally infected with different ASFV-genotype isolate. Shown are ASFV-P72 transcript-specific Cq-values of third nymphal stage ticks fed with defibrinated pig blood…
View article: Additional file 17 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 17 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 17: Table S11. O. porcinus piRNA ping-pong signature analysed by PingPongPro v1.0 with default parameters and -b option (creates additional browser track files, which are suitable for display in common genome browsers). The…
View article: Additional file 3 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 3 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 3: Table S3. BLASTn results of SPAdes-assembled contigs containing ASFLI- and other endogenous viral elements detected in the OME/CTVM21 genome. Shown are hits with the lowest e-value for viral, tick and mobile genetic elem…
View article: Additional file 9 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 9 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 9: Table S7. SPAdes-assembled contigs from deep sequencing data from libraries AGL001 and MPA001, as generated from museum-stored ticks. Data from libraries from museum-stored tick were mapped against ASFLI-element-containi…
View article: Additional file 4 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution
Additional file 4 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution Open
Additional file 4: Table S4. BLASTp results of all ORFs >500 bp on SPAdes-assembled contigs containing ASFLI- and other endogenous viral elements detected in the OME/CTVM21 genome Shown are hits with the lowest e-value for viral-, tick and…
View article: African Swine Fever in a Bulgarian Backyard Farm—A Case Report
African Swine Fever in a Bulgarian Backyard Farm—A Case Report Open
African swine fever (ASF) is one of the most threatening diseases for the pig farming sector worldwide. As an effective vaccine is lacking, strict application of control measures is the only way to fight the disease in both industrial farm…