Jan Löwe
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View article: Molecular structure of the ESCRT III-based archaeal CdvAB cell division machinery
Molecular structure of the ESCRT III-based archaeal CdvAB cell division machinery Open
Most prokaryotes divide using filaments of the tubulin-like FtsZ protein, while some archaea employ instead ESCRT-III-like proteins and their filaments for cell division and cytokinesis. The alternative archaeal system comprises Cdv protei…
View article: Foam film vitrification for cryo-EM
Foam film vitrification for cryo-EM Open
Electron cryomicroscopy (cryo-EM) has revolutionised structural biology, enhancing applicability, size limits and speed. Despite these successes, cryo-EM sample preparation remains a major bottleneck for routinely achieving high-resolution…
View article: Foam film vitrification for cryo-EM
Foam film vitrification for cryo-EM Open
Electron cryomicroscopy (cryo-EM) has revolutionised structural biology, enhancing applicability, size limits and speed. Despite these successes, cryo-EM sample preparation remains a major bottleneck for routinely achieving high-resolution…
View article: Mechanism of DNA entrapment by the MukBEF SMC complex and its inhibition by a viral DNA mimic
Mechanism of DNA entrapment by the MukBEF SMC complex and its inhibition by a viral DNA mimic Open
Summary Ring-like structural maintenance of chromosomes (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC …
View article: Honeycomb gold specimen supports enabling orthogonal focussed ion beam-milling of elongated cells for cryo-ET
Honeycomb gold specimen supports enabling orthogonal focussed ion beam-milling of elongated cells for cryo-ET Open
Cryo-focussed ion beam (FIB)-milling is a powerful technique that opens up thick, cellular specimens to high-resolution structural analysis by electron cryotomography (cryo-ET). FIB-milled lamellae can be produced from cells on grids, or c…
View article: Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division
Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division Open
Cell division in all domains of life requires the orchestration of many proteins, but in Archaea most of the machinery remains poorly characterized. Here we investigate the FtsZ-based cell division mechanism in Haloferax volcanii and find …
View article: Filament structure and subcellular organization of the bacterial intermediate filament–like protein crescentin
Filament structure and subcellular organization of the bacterial intermediate filament–like protein crescentin Open
The protein crescentin is required for the crescent shape of the freshwater bacterium Caulobacter crescentus ( vibrioides ). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features with e…
View article: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: Author Response: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
Author Response: What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Full text Figures and data Side by side Abstract eLife assessment Introduction Results Discussion Methods Data availability References Peer review Author response Article and author information Metrics Abstract Cohesin is a trimeric comple…
View article: Reviewer #2 (Public Review): What AlphaFold tells us about cohesin’s retention on and release from chromosomes
Reviewer #2 (Public Review): What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: Reviewer #1 (Public Review): What AlphaFold tells us about cohesin’s retention on and release from chromosomes
Reviewer #1 (Public Review): What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: Author Response: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
Author Response: What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: Author Response: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
Author Response: What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin
Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin Open
The coiled coil protein crescentin is required for the crescent shape of the freshwater bacterium Caulobacter crescentus ( vibrioides ). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares fea…
View article: Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL
Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL Open
In most bacteria, cell division relies on the synthesis of new cell wall material by the multiprotein divisome complex. Thus, at the core of the divisome are the transglycosylase FtsW, which synthesises peptidoglycan strands from its subst…
View article: Structural biology of SMC complexes across the tree of life
Structural biology of SMC complexes across the tree of life Open
Structural maintenance of chromosomes (SMC) complexes guard and organize the three-dimensional structure of chromosomal DNA across the tree of life. Many SMC functions can be explained by an inherent motor activity that extrudes large DNA …
View article: What AlphaFold tells us about cohesin’s retention on and release from chromosomes
What AlphaFold tells us about cohesin’s retention on and release from chromosomes Open
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1. During interphase, it organizes chromosomal DNA topology by extruding loo…
View article: Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics
Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics Open
Protein filaments are used in myriads of ways to organize other molecules within cells. Some filament-forming proteins couple the hydrolysis of nucleotides to their polymerization cycle, thus powering the movement of other molecules. These…
View article: PRC domain-containing proteins modulate FtsZ-based archaeal cell division
PRC domain-containing proteins modulate FtsZ-based archaeal cell division Open
Dividing cells into two daughter cells is a complicated process that in bacteria and eukaryotes requires many proteins to work together. For archaea that divide via an FtsZ-based mechanism, only three proteins of the cell division machiner…
View article: The patterned assembly and stepwise Vps4-mediated disassembly of composite ESCRT-III polymers drives archaeal cell division
The patterned assembly and stepwise Vps4-mediated disassembly of composite ESCRT-III polymers drives archaeal cell division Open
ESCRT-III family proteins form composite polymers that deform and cut membrane tubes in the context of a wide range of cell biological processes across the tree of life. In reconstituted systems, sequential changes in the composition of ES…
View article: AlphaFold predictions for Nasmyth et al., 2023
AlphaFold predictions for Nasmyth et al., 2023 Open
AlphaFold predictions as described in Nasmyth et al., 2023.
View article: AlphaFold predictions for Nasmyth et al., 2023
AlphaFold predictions for Nasmyth et al., 2023 Open
AlphaFold predictions as described in Nasmyth et al., 2023.
View article: Divisome core complex in bacterial cell division revealed by cryo-EM
Divisome core complex in bacterial cell division revealed by cryo-EM Open
Cell division, or cytokinesis is a fundamental process of life and, in most bacteria, is driven by peptidoglycan synthesis at the septum 1 . It is catalysed by the divisome, a multi-protein complex with more than 20 components that spans t…