Jan Paleček
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View article: The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3
The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3 Open
The centromere is a specialized domain that facilitates chromosome segregation during mitosis and serves as the site for kinetochore formation. KINETOCHORE NULL2 (αKNL2) is essential for the recognition and loading of the centromeric histo…
View article: Homologous recombination mutant lethality differs between <i>h</i>− and <i>h+ Schizosaccharomyces pombe</i> strains due to <i>mat1</i> heterochromatin
Homologous recombination mutant lethality differs between <i>h</i>− and <i>h+ Schizosaccharomyces pombe</i> strains due to <i>mat1</i> heterochromatin Open
Homologous recombination (HR) is generally considered dispensable in yeast and vertebrates, yet mounting evidence indicates that its essentiality depends on cellular context. Here, we dissect the basis of this context dependency in Schizos…
View article: The Phenotype of Physcomitrium patens SMC6 Mutant with Interrupted Hinge Interactions
The Phenotype of Physcomitrium patens SMC6 Mutant with Interrupted Hinge Interactions Open
Background/Objectives: The Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct inter…
View article: The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3
The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3 Open
The centromere is a specialized domain that facilitates chromosome segregation during mitosis and serves as the site for kinetochore formation. KINETOCHORE NULL2 (αKNL2) is essential for the recognition and loading of the centromeric histo…
View article: Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions
Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions Open
Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, w…
View article: The phenotype of SMC6_G514R hinge mutant of<i>Physcomitrium patents</i>
The phenotype of SMC6_G514R hinge mutant of<i>Physcomitrium patents</i> Open
The Structural Maintenance of Chromosomes (SMC) complexes play roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of SMC proteins with a unique structure consisting of an ATPase head, long…
View article: NSE5 subunit interacts with distant regions of the <scp>SMC</scp> arms in the <i>Physcomitrium patens</i> SMC5/6 complex
NSE5 subunit interacts with distant regions of the <span>SMC</span> arms in the <i>Physcomitrium patens</i> SMC5/6 complex Open
SUMMARY Structural maintenance of chromosome (SMC) complexes play roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of SMC proteins with a unique structure consisting of an ATPase head, l…
View article: The interplay of homology‐directed repair pathways in the repair of zebularine‐induced <scp>DNA</scp>–protein crosslinks in Arabidopsis
The interplay of homology‐directed repair pathways in the repair of zebularine‐induced <span>DNA</span>–protein crosslinks in Arabidopsis Open
SUMMARY DNA–protein crosslinks (DPCs) are highly toxic DNA lesions represented by proteins covalently bound to the DNA. Persisting DPCs interfere with fundamental genetic processes such as DNA replication and transcription. Cytidine analog…
View article: Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex
Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex Open
View article: Correction: Kolesar et al. Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase. Cells 2022, 11, 165
Correction: Kolesar et al. Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase. Cells 2022, 11, 165 Open
In the original publication [...]
View article: NSE5 subunit interacts with distant regions of the SMC arms in the<i>Physcomitrium patens</i>SMC5/6 complex
NSE5 subunit interacts with distant regions of the SMC arms in the<i>Physcomitrium patens</i>SMC5/6 complex Open
Structural Maintenance of Chromosome (SMC) complexes play roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of SMC proteins with a unique structure consisting of an ATPase head, long arm,…
View article: Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions
Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions Open
Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as …
View article: The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
The SAGA histone acetyltransferase module targets SMC5/6 to specific genes Open
View article: Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions
Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions Open
Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as …
View article: Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis
Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis Open
The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identi…
View article: Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions
Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions Open
Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as …
View article: SAGA histone acetyltransferase module facilitates chromatin accessibility to SMC5/6
SAGA histone acetyltransferase module facilitates chromatin accessibility to SMC5/6 Open
Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin, and SMC5/6) play key roles in cohesion, condensation, rep…
View article: Characterization of the NSE6 subunit of the<i>Physcomitrium patens</i>PpSMC5/6 complex
Characterization of the NSE6 subunit of the<i>Physcomitrium patens</i>PpSMC5/6 complex Open
Structural Maintenance of Chromosome (SMC) complexes are molecular machines ensuring chromatin organization at higher levels. They play direct roles in cohesion, condensation, replication, transcription and DNA repair. Their cores are comp…
View article: Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase
Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase Open
Structural Maintenance of Chromosomes (SMC) complexes are important for many aspects of the chromosomal organization. Unlike cohesin and condensin, the SMC5/6 complex contains a variant RING domain carried by its Nse1 subunit. RING domains…
View article: A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6
A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6 Open
View article: A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6
A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6 Open
The SMC (Structural Maintenance of Chromosomes) complexes are composed of SMC dimers, kleisin and kleisin-interacting subunits. Mutual interactions of these subunits constitute the basal architecture of the SMC complexes. Particularly, ter…
View article: A Single Conserved Amino Acid Residue as a Critical Context-Specific Determinant of the Differential Ability of Mdm2 and MdmX RING Domains to Dimerize
A Single Conserved Amino Acid Residue as a Critical Context-Specific Determinant of the Differential Ability of Mdm2 and MdmX RING Domains to Dimerize Open
Mdm2 and MdmX are related proteins serving in the form of the Mdm2 homodimer or Mdm2/MdmX heterodimer as an E3 ubiquitin ligase for the tumor suppressor p53. The dimerization is required for the E3 activity and is mediated by the conserved…
View article: SMC5/6: Multifunctional Player in Replication
SMC5/6: Multifunctional Player in Replication Open
The genome replication process is challenged at many levels. Replication must proceed through different problematic sites and obstacles, some of which can pause or even reverse the replication fork (RF). In addition, replication of DNA wit…
View article: COZOID: contact zone identifier for visual analysis of protein-protein interactions
COZOID: contact zone identifier for visual analysis of protein-protein interactions Open
We developed a system for the exploration of large sets of protein-protein complexes in a fast and intuitive way. The usefulness of our system has been tested and verified on several docking structures covering the three major types of PPI…
View article: Additional file 3 of COZOID: contact zone identifier for visual analysis of protein-protein interactions
Additional file 3 of COZOID: contact zone identifier for visual analysis of protein-protein interactions Open
Example data. Testing dataset used in the manuscript. (ZIP 2662 kb)
View article: Additional file 2 of COZOID: contact zone identifier for visual analysis of protein-protein interactions
Additional file 2 of COZOID: contact zone identifier for visual analysis of protein-protein interactions Open
Software build. Executable binary file of the software tool. (ZIP 118,784 kb)
View article: Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease
Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease Open
The structural maintenance of chromosomes (SMC) family of proteins supports mitotic proliferation, meiosis, and DNA repair to control genomic stability. Impairments in chromosome maintenance are linked to rare chromosome breakage disorders…
View article: Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes
Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes Open
View article: Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA
Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA Open
SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication for…
View article: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex Open
The MAGE (Melanoma-associated antigen) protein family members are structurally related to each other by a MAGE-homology domain comprised of 2 winged helix motifs WH/A and WH/B. This family specifically evolved in placental mammals although…