Jan Tkáč
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Glycoproteomics of Gastrointestinal Cancers and Its Use in Clinical Diagnostics Open
Cancer is a leading cause of death worldwide, resulting in substantial economic costs. Because cancer is a complex, heterogeneous group of diseases affecting a variety of cells, its detection may sometimes be difficult. Herein we review a …
Cutting‐Edge Aptasensing Approaches for Electrochemical Detection of Exosomes Utilizing MXenes Open
Herein, the basic properties, composition, and isolation methods for the separation of exosomes are described, since they can be a rich source of disease biomarkers. Then an introduction to MXenes, a novel class of 2D nanomaterials with in…
Advances in magnetic affinity-based isolation/detection of exosomes for robust diagnostics Open
The review article provides a short introduction to exosomes with the focus to use exosomes as disease markers itself (i.e. their concentration or presence of some specific receptors) or a source of disease biomarkers such as proteins and …
Direct electron transfer and direct bioelectrocatalysis of sarcosine oxidase on Ti3C2Tx MXene hybrid interface Open
The current study shows a direct electron transfer (DET) and direct bioelectrocatalysis of the sarcosine oxidase (SOx) on the screen-printed carbon electrode (SPCE) modified by a hybrid bionanocomposite composed of chitosan (CS) and Ti 3 C…
N-Acetylated Monosaccharides and Derived Glycan Structures Occurring in N- and O-Glycans During Prostate Cancer Development Open
Post-translational modifications of proteins play an important role in their stability, solubility and in vivo function. Also, for several reasons, such as the Golgi fragmentation during cancerogenesis, glycosylation as the most common mod…
Serum levels of prostate specific antigen, free PSA, [−2]proPSA, fPSA/tPSA ratio, Prostate Health Index, and glycosylation patterns of free PSA in patients with benign prostatic hyperplasia pharmacotherapy Open
Background The medication used to treat benign prostate hyperplasia (BPH), a common condition in men over 50 years of age, can alter the levels of biomarkers used in prostate cancer detection. Commonly used medications for BPH include alph…
View article: What Are the Key Factors for the Detection of Peptides Using Mass Spectrometry on Boron-Doped Diamond Surfaces?
What Are the Key Factors for the Detection of Peptides Using Mass Spectrometry on Boron-Doped Diamond Surfaces? Open
We investigated the use of boron-doped diamond (BDD) with different surface morphologies for the enhanced detection of nine different peptides by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). For the first time,…
Medical Relevance, State-of-the-Art and Perspectives of “Sweet Metacode” in Liquid Biopsy Approaches Open
This review briefly introduces readers to an area where glycomics meets modern oncodiagnostics with a focus on the analysis of sialic acid (Neu5Ac)-terminated structures. We present the biochemical perspective of aberrant sialylation durin…
View article: Glycoprofiling of proteins as prostate cancer biomarkers: A multinational population study
Glycoprofiling of proteins as prostate cancer biomarkers: A multinational population study Open
The glycoprofiling of two proteins, the free form of the prostate-specific antigen (fPSA) and zinc-α-2-glycoprotein (ZA2G), was assessed to determine their suitability as prostate cancer (PCa) biomarkers. The glycoprofiling of proteins was…
Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker Open
Prostate cancer (PCa) is the second most common cancer. In this paper, the isolation and properties of exosomes as potential novel liquid biopsy markers for early PCa liquid biopsy diagnosis are investigated using two prostate human cell l…
View article: Glycoprofiling of proteins as prostate cancer biomarkers: a multinational population study
Glycoprofiling of proteins as prostate cancer biomarkers: a multinational population study Open
The glycoprofiling of two proteins, the free form of the prostate-specific antigen (fPSA) and zinc-α-2-glycoprotein (ZA2G), was assessed to determine their suitability as prostate cancer (PCa) biomarkers. The glycoprofiling of proteins was…
Amperometric Miniaturised Portable Enzymatic Nanobiosensor for the Ultrasensitive Analysis of a Prostate Cancer Biomarker Open
Screen-printing technology is a game changer in many fields including electrochemical biosensing. Two-dimensional nanomaterial MXene Ti3C2Tx was integrated as a nanoplatform to immobilise enzyme sarcosine oxidase (SOx) onto the interface o…
Exosomes from prostate cancer cell lines: Isolation optimisation and characterisation Open
Exosomes are considered to be a rich source of biomarkers, hence in this article we examine the best procedure for their isolation. We examine several isolation procedures, exosome storage conditions and other conditions affecting exosome …
Glycan signatures for the identification of cisplatin‐resistant testicular cancer cell lines: Specific glycoprofiling of human chorionic gonadotropin (hCG) Open
Background Testicular cancer (TC) is the most frequent type of cancer among young men aged between 15 and 34 years. TC is treated using cisplatin, but 3%–5% of TC patients fail to respond to cisplatin, with a very bad to fatal prognosis. A…
Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray Open
Colorectal cancer (CRC) is one of the most common types of cancer among men and women worldwide. Efforts are currently underway to find novel and more cancer-specific biomarkers that could be detected in a non-invasive way. The analysis of…
Tandem Osmotic Engine Based on Hydrogel Particles with Antipolyelectrolyte and Polyelectrolyte Effect Fuelled by Both Salinity Gradient Modes Open
In this study, we propose a new approach to attain energy by salinity gradient engines with pistons based on hydrogels possessing polyelectrolyte and antipolyelectrolyte effects in a tandem arrangement, providing energy in each salinity gr…
Exchange Counterion in Polycationic Hydrogels: Tunability of Hydrophobicity, Water State, and Floating Capability for a Floating pH Device Open
Smart gel materials are capable of controlling and switching swelling, water state, and wettability properties triggered by external stimuli. In this study, we fabricated a series of polyelectrolyte hydrogels bearing a 3-trimethylammoniump…
Novel Prostate Cancer Biomarkers: Aetiology, Clinical Performance and Sensing Applications Open
The review initially provides a short introduction to prostate cancer (PCa) incidence, mortality, and diagnostics. Next, the need for novel biomarkers for PCa diagnostics is briefly discussed. The core of the review provides details about …
Nicotinamide-based supergelator self-assembling via asymmetric hydrogen bonding NH⋯OC and H⋯Br− pattern for reusable, moldable and self-healable nontoxic fuel gels Open
Nanostructural architecture of the network depended on the solvent used and showed variations in size and shape of 1D nanofibers. Supergelation is attributed to a unique asymmetric NH⋯OC, H⋯Br- hydrogen bonding pattern between H-2 hydrogen…
Ti<sub>3</sub>C<sub>2</sub> MXene-Based Nanobiosensors for Detection of Cancer Biomarkers Open
This chapter provides information about basic properties of MXenes (2D nanomaterials) that are attractive for a design of various types of nanobiosensors. The second part of the chapter discusses MXene synthesis and various protocols for m…
Influence of direct electric field on PMCG-alginate-based microcapsule Open
In this study, the influence of direct electric current on a microcapsule was investigated. The microcapsule consisted of a core from a calcium ion and sodium alginate (SA) complex and the microcapsule membrane was formed by the polyionic …
SENSITIVE AMPEROMETRIC NANOBIOSENSOR FOR DETECTION OF SARCOSINE - POTENTIAL PROSTATE CANCER MARKER - IN URINE SAMPLES Open
In this work, two-dimensional layered material Ti3C2TX MXene has been used to immobilize enzyme sarcosine oxidase to fabricate a nanostructured biosensor.The device was applied for detection of sarcosine, a potential prostate cancer biomar…
screen-printed molybdenum disulfide electrodes for electrochemical sensing of dopamine Open
Two-dimensional (2D) molybdenum disulphide (MoS2) belongs to a class of materials called transition metal dichalcogenides (TMDCs).Due to its large surface area, high biocompatibility, non-zero band-gap, structural versatility and mechanica…
Electrochemical Impedance Spectroscopy on 2D Nanomaterial MXene Modified Interfaces: Application as a Characterization and Transducing Tool Open
This review presents the basic characteristics of MXene, a novel 2D nanomaterial with many outstanding properties applicable to electrochemical sensing and biosensing. The second part deals with electrochemical impedance spectroscopy (EIS)…