K. Jane Grande‐Allen
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View article: Aberrant Regulation of endMT in Turner Syndrome: Implications for the Pathogenesis of Congenital Cardiovascular Disease
Aberrant Regulation of endMT in Turner Syndrome: Implications for the Pathogenesis of Congenital Cardiovascular Disease Open
Turner syndrome (TS) is caused by the partial/complete absence of one sex chromosome and affects 1 in 2500 liveborn infants. Bicuspid aortic valve, and thoracic aortic aneurysms are more prevalent in TS compared to the general population. …
View article: Comparative Analysis of the Endothelial Glycocalyx in Aortic Valve and Vascular Endothelial Cells Under Distinct Shear Stress Conditions
Comparative Analysis of the Endothelial Glycocalyx in Aortic Valve and Vascular Endothelial Cells Under Distinct Shear Stress Conditions Open
Background: The endothelial glycocalyx (EG) regulates vascular permeability, inflammation, and mechanotransduction. While the EG has been studied in vascular endothelial cells (VECs), less is known about its behavior in aortic valve endoth…
View article: Shear-Induced Macrophage Secretome Promotes Endothelial Permeability
Shear-Induced Macrophage Secretome Promotes Endothelial Permeability Open
Background Discrete subaortic stenosis is a pediatric cardiovascular disease marked by fibrotic growth within the left ventricular outflow tract (LVOT), leading to severe complications, including left ventricular hypertrophy, aortic regurg…
View article: Shear Stress Conditioning Promotes a Pro-Inflammatory Response in Porcine Endocardial Endothelial Cells
Shear Stress Conditioning Promotes a Pro-Inflammatory Response in Porcine Endocardial Endothelial Cells Open
PURPOSE Discrete subaortic stenosis (DSS) is a congenital heart disease characterized by a narrowing of the passage below the aortic valve in the left ventricular outflow tract (LVOT) [1]. While endocardial endothelial cells (EECs) are kno…
View article: Shear Stress Modulates Inflammatory Responses in Porcine Endothelial Cells Contributing to the Fibrotic Response of Porcine Cardiac Fibroblasts
Shear Stress Modulates Inflammatory Responses in Porcine Endothelial Cells Contributing to the Fibrotic Response of Porcine Cardiac Fibroblasts Open
This study is focused on discrete subaortic stenosis (DSS) which is a congenital heart disease resulting in the formation of fibrotic tissue in the left ventricular outflow track of pediatric patients [1]. The pathogenesis of the molecular…
View article: Development of a novel <i>In Vitro</i> Co-culture system for discrete subaortic stenosis
Development of a novel <i>In Vitro</i> Co-culture system for discrete subaortic stenosis Open
View article: A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction
A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction Open
Mechanistic investigation of host-microbe interactions in the human gut are hindered by difficulty of co-culturing microbes with intestinal epithelial cells. On one hand the gut bacteria are a mix of facultative, aerotolerant or obligate a…
View article: Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers
Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers Open
Progress in the design and synthesis of nanostructured self-assembling systems has facilitated the realization of numerous nanoscale geometries, including fibers, ribbons, and sheets. A key challenge has been achieving control across multi…
View article: Experimental multiparametric magnetic resonance imaging characterization of iliocaval venous thrombosis pathological changes
Experimental multiparametric magnetic resonance imaging characterization of iliocaval venous thrombosis pathological changes Open
View article: Assessing Drivers of Gender Balance and Racial Makeup of Editorial Board Members in Biomedical Engineering
Assessing Drivers of Gender Balance and Racial Makeup of Editorial Board Members in Biomedical Engineering Open
Scientific journal editors serve as gatekeepers with the decision-making power of assigning reviewers to manuscripts. Serving as an editor is also an important stage in a young academic’s career progression, and an indicator of high regard…
View article: Technique for Rapidly Forming Networks of Microvessel-Like Structures
Technique for Rapidly Forming Networks of Microvessel-Like Structures Open
Modeling organ-blood barriers through the inclusion of microvessel networks within in vitro tissue models could lead to more physiologically accurate results, especially since organ-blood barriers are crucial to the normal function,…
View article: Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers
Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers Open
Fibrous proteins that comprise the extracellular matrix (ECM) guide cellular growth and tissue organization. A lack of synthetic strategies able to generate aligned, ECM-mimetic biomaterials has hampered bottom-up tissue engineering of ani…
View article: Grand Challenges at the Interface of Engineering and Medicine
Grand Challenges at the Interface of Engineering and Medicine Open
Over the past two decades Biomedical Engineering has emerged as a major discipline that bridges societal needs of human health care with the development of novel technologies. Every medical institution is now equipped at varying degrees of…
View article: Assessment of spinal cord injury using ultrasound elastography in a rabbit model in vivo
Assessment of spinal cord injury using ultrasound elastography in a rabbit model in vivo Open
View article: Development of Novel Multicellular 3D Spheroids for Discrete Subaortic Stenosis
Development of Novel Multicellular 3D Spheroids for Discrete Subaortic Stenosis Open
In this study, we propose a new method for bioprinting 3D Spheroids to study complex congenital heart disease known as discrete subaortic stenosis (DSS). The bioprinter allows us to manipulate the extrusion pressure to change the size of t…
View article: Development of a Novel In Vitro Co-Culture System for Discrete Subaortic Stenosis
Development of a Novel In Vitro Co-Culture System for Discrete Subaortic Stenosis Open
The aim of this study was to investigate the survival, distribution and reaction of different cell types on a monolayer disk, as well as their behavior under bioreactor treatment. Specifically, porcine EEC and porcine fibroblasts (PCF) wer…
View article: Technique for rapidly forming networks of microvessel-like structures
Technique for rapidly forming networks of microvessel-like structures Open
Modelling organ-blood barriers through the inclusion of microvessel networks within in vitro tissue models could lead to more physiologically accurate results, especially since organ-blood barriers are crucial to the normal function, drug …
View article: Isolation and Characterization of Porcine Endocardial Endothelial Cells
Isolation and Characterization of Porcine Endocardial Endothelial Cells Open
The heart contains diverse endothelial cell types. We sought to characterize the endocardial endothelial cells (EECs), which line the chambers of the heart. EECs are relatively understudied, yet their dysregulation can lead to various card…
View article: Supplementary movie 2 Adjacent tumor spheroids disorganize and migrate on CAF networks from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Supplementary movie 2 Adjacent tumor spheroids disorganize and migrate on CAF networks from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma Open
A Matrigel surface was seeded first with GFP-tagged aggregates followed 24 h later by a CAF single-cell suspension. Time-lapse microscopy was initiated 24 hours after CAF seeding. Dissociating tumor aggregates migrate toward each other (ar…
View article: Supplementary movie 2 Adjacent tumor spheroids disorganize and migrate on CAF networks from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Supplementary movie 2 Adjacent tumor spheroids disorganize and migrate on CAF networks from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma Open
A Matrigel surface was seeded first with GFP-tagged aggregates followed 24 h later by a CAF single-cell suspension. Time-lapse microscopy was initiated 24 hours after CAF seeding. Dissociating tumor aggregates migrate toward each other (ar…
View article: Data from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Data from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma Open
Intratumoral collagen cross-links heighten stromal stiffness and stimulate tumor cell invasion, but it is unclear how collagen cross-linking is regulated in epithelial tumors. To address this question, we used KrasLA1 mice, whic…
View article: Supplementary movie 1 CAFs spontaneously organize into networks with contractile properties from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Supplementary movie 1 CAFs spontaneously organize into networks with contractile properties from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma Open
A Matrigel surface was seeded first with GFP-tagged aggregates followed 24 h later by a CAF single-cell suspension. Time-lapse microscopy was immediately after CAF seeding. Scale bar, 200 μm. Movie compiled at 8 frames per second.
View article: Supplementary movie 1 CAFs spontaneously organize into networks with contractile properties from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Supplementary movie 1 CAFs spontaneously organize into networks with contractile properties from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma Open
A Matrigel surface was seeded first with GFP-tagged aggregates followed 24 h later by a CAF single-cell suspension. Time-lapse microscopy was immediately after CAF seeding. Scale bar, 200 μm. Movie compiled at 8 frames per second.
View article: Data from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Data from Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma Open
Intratumoral collagen cross-links heighten stromal stiffness and stimulate tumor cell invasion, but it is unclear how collagen cross-linking is regulated in epithelial tumors. To address this question, we used KrasLA1 mice, whic…
View article: Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptor
Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptor Open
Calcific aortic valve disease (CAVD), a fibrocalcific thickening of the aortic valve leaflets causing obstruction of the left ventricular outflow tract, affects nearly 10 million people worldwide. For those who reach end-stage CAVD, the on…
View article: Genomic and Proteomic Analysis of Patients with Reoccurring Discrete Subaortic Stenosis
Genomic and Proteomic Analysis of Patients with Reoccurring Discrete Subaortic Stenosis Open
Discrete subaortic stenosis (DSS) is a pediatric condition in which a fibrotic membrane forms within the left ventricular outflow track. The fibrotic membrane is removed surgically; however, there is a high rate of reoccurrence which requi…
View article: Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering
Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering Open
Demineralized bone matrix (DBM) has been widely used clinically for dental, craniofacial and skeletal bone repair, as an osteoinductive and osteoconductive material. 3D printing (3DP) enables the creation of bone tissue engineering scaffol…
View article: Erratum to “BME 2.0: Engineering the Future of Medicine”
Erratum to “BME 2.0: Engineering the Future of Medicine” Open
[This corrects the article DOI: 10.34133/bmef.0001.].
View article: BME 2.0: Engineering the Future of Medicine
BME 2.0: Engineering the Future of Medicine Open
If the 20th century was the age of mapping and controlling the external world, the 21st century is the biomedical age of mapping and controlling the biological internal world. The biomedical age is bringing new technological breakthroughs …
View article: Shear Stress Induces a Time-Dependent Inflammatory Response in Human Monocyte-Derived Macrophages
Shear Stress Induces a Time-Dependent Inflammatory Response in Human Monocyte-Derived Macrophages Open
Macrophages are innate immune cells that are known for their extreme plasticity, enabling diverse phenotypes that lie on a continuum. In a simplified model, they switch between pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes de…