Janet L. Smith
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View article: Investigating Macrolactone Formation by Thioesterase Domains through Incorporation of an Unnatural Amino Acid
Investigating Macrolactone Formation by Thioesterase Domains through Incorporation of an Unnatural Amino Acid Open
Vishakha Choudhary, Tyler M. McCullough, David L. Akey, Meredith A. Skiba, Steffen M. Bernard, Jeffrey D. Kittendorf, Jennifer J. Schmidt, David H. Sherman and Janet L. Smith Life Sciences Institute, Department of Biological Chemistry, Dep…
View article: Returning to scientific operations at GM/CA@APS after the APS-Upgrade
Returning to scientific operations at GM/CA@APS after the APS-Upgrade Open
The Advanced Photon Source (APS) underwent a comprehensive upgrade to replace the original electron storage ring with a new storage ring (APS-U) that increased the X-ray brightness by up to 500 times compared to the APS. The National Insti…
View article: In These Times
In These Times Open
View article: Structure of a putative terminal amidation domain in natural product biosynthesis
Structure of a putative terminal amidation domain in natural product biosynthesis Open
View article: Redefining the Role of the EryM Acetyltransferase in Natural Product Biosynthetic Pathways
Redefining the Role of the EryM Acetyltransferase in Natural Product Biosynthetic Pathways Open
Summary The GNAT (GCN5-related N -acetyltransferase) superfamily comprises enzymes with a conserved fold and diverse catalytic activities, including primarily acyl transfer, with a few examples of decarboxylation. EryM, a GNAT enzyme from …
View article: The renewed GM/CA@APS Structural Biology Facility and APS-Upgrade
The renewed GM/CA@APS Structural Biology Facility and APS-Upgrade Open
The Advanced Photon Source (APS) has undergone a comprehensive upgrade to replace the original electron storage ring with a new storage ring (APS-U) that will increase the X-ray brightness 500 times compared to the APS. The National Instit…
View article: Structural Study of HIV-1 Reverse Transcription Initiation
Structural Study of HIV-1 Reverse Transcription Initiation Open
Acquired Immunodeficiency Syndrome (AIDS) caused by Human Immunodeficiency Virus (HIV) is a public health threat worldwide. Currently, there is no effective cure for HIV-AIDS. The long-term survival of HIV-infected individuals requires lif…
View article: Serendipitous high-resolution structure of <i>Escherichia coli</i> carbonic anhydrase 2
Serendipitous high-resolution structure of <i>Escherichia coli</i> carbonic anhydrase 2 Open
X-ray crystallography remains the dominant method of determining the three-dimensional structure of proteins. Nevertheless, this resource-intensive process may be hindered by the unintended crystallization of contaminant proteins from the …
View article: Functional anatomy of zinc finger antiviral protein complexes
Functional anatomy of zinc finger antiviral protein complexes Open
View article: A minimal complex of KHNYN and zinc-finger antiviral protein binds and degrades single-stranded RNA
A minimal complex of KHNYN and zinc-finger antiviral protein binds and degrades single-stranded RNA Open
Detecting viral infection is a key role of the innate immune system. The genomes of some RNA viruses have a high CpG dinucleotide content relative to most vertebrate cell RNAs, making CpGs a molecular marker of infection. The human zinc-fi…
View article: Structure of a Putative Terminal Amidation Domain in Natural Product Biosynthesis
Structure of a Putative Terminal Amidation Domain in Natural Product Biosynthesis Open
SUMMARY Bacteria are rich sources of pharmaceutically valuable natural products, many crafted by modular polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS). PKS and NRPS systems typically contain a thioesterase (TE) to…
View article: Substrate Trapping in Polyketide Synthase Thioesterase Domains: Structural Basis for Macrolactone Formation
Substrate Trapping in Polyketide Synthase Thioesterase Domains: Structural Basis for Macrolactone Formation Open
Emerging antibiotic resistance requires continual improvement in the arsenal of antimicrobial drugs, especially the critical macrolide antibiotics. Formation of the macrolactone scaffold of these polyketide natural products is catalyzed by…
View article: Substrate Trapping in Polyketide Synthase Thioesterase Domains: Structural Basis for Macrolactone Formation
Substrate Trapping in Polyketide Synthase Thioesterase Domains: Structural Basis for Macrolactone Formation Open
Emerging antibiotic resistance requires continual improvement in the arsenal of antimicrobial drugs, especially the critical macrolide antibiotics. Formation of the macrolactone scaffold of these polyketide natural products is catalyzed by…
View article: Real-World Treatment Patterns and Clinical Outcomes among Patients Receiving CDK4/6 Inhibitors for Metastatic Breast Cancer in a Canadian Setting Using AI-Extracted Data
Real-World Treatment Patterns and Clinical Outcomes among Patients Receiving CDK4/6 Inhibitors for Metastatic Breast Cancer in a Canadian Setting Using AI-Extracted Data Open
Cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) are widely used in patients with hormone receptor-positive (HR+)/human epidermal growth factor receptor 2 negative (HER2−) advanced/metastatic breast cancer (ABC/MBC) in first line (1L), but…
View article: An intramolecular macrocyclase in plant ribosomal peptide biosynthesis
An intramolecular macrocyclase in plant ribosomal peptide biosynthesis Open
View article: Iron Surveillance and Management in Gastro-Intestinal Oncology Patients: A National Physician Survey
Iron Surveillance and Management in Gastro-Intestinal Oncology Patients: A National Physician Survey Open
Purpose: Iron deficiency (ID) is a complication of gastrointestinal (GI) cancers that may manifest as iron deficiency anemia (IDA). Serum ferritin monitoring and oral iron supplementation have the limitations of being falsely elevated and …
View article: PO107 TREATMENT PATTERNS AND CLINICAL OUTCOMES AMONG PATIENTS RECEIVING CDK4/6 INHIBITORS FOR HR+/HER2-ADVANCED/METASTATIC BREAST CANCER IN A SINGLE-CENTRE, CANADIAN REAL-WORLD SETTING USING AI-EXTRACTED DATA
PO107 TREATMENT PATTERNS AND CLINICAL OUTCOMES AMONG PATIENTS RECEIVING CDK4/6 INHIBITORS FOR HR+/HER2-ADVANCED/METASTATIC BREAST CANCER IN A SINGLE-CENTRE, CANADIAN REAL-WORLD SETTING USING AI-EXTRACTED DATA Open
View article: Commentary on `Facing the phase problem' by Wayne Hendrickson
Commentary on `Facing the phase problem' by Wayne Hendrickson Open
A commentary on Wayne Hendrickson's article `Facing the phase problem'.
View article: Structure of a modular polyketide synthase reducing region
Structure of a modular polyketide synthase reducing region Open
View article: Deciphering the evolution of flavin-dependent monooxygenase stereoselectivity using ancestral sequence reconstruction
Deciphering the evolution of flavin-dependent monooxygenase stereoselectivity using ancestral sequence reconstruction Open
Controlling the selectivity of a reaction is critical for target-oriented synthesis. Accessing complementary selectivity profiles enables divergent synthetic strategies, but is challenging to achieve in biocatalytic reactions given enzymes…
View article: Natural killer cytotoxicity in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS): a multi-site clinical assessment of ME/CFS (MCAM) sub-study
Natural killer cytotoxicity in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS): a multi-site clinical assessment of ME/CFS (MCAM) sub-study Open
Background Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a multisystem illness characterized by substantial reduction in function accompanied by profound unexplained fatigue not significantly relieved by rest, post-exertio…
View article: Effectiveness variation of different census outreach activities: An empirical analysis from the state of Illinois using machine learning and user interface technologies for participatory data collection
Effectiveness variation of different census outreach activities: An empirical analysis from the state of Illinois using machine learning and user interface technologies for participatory data collection Open
View article: Feasibility of Mindfulness-Based Intervention for Veterans Managing Chronic Pain
Feasibility of Mindfulness-Based Intervention for Veterans Managing Chronic Pain Open
Background. Chronic pain impacts people's activity participation, productivity, mental health, and sense of wellbeing. Purpose. This study aimed to (1) evaluate the feasibility of the Mindfulness-Based Chronic Pain Management (MBCPM TM ) p…
View article: Data Science-Driven Analysis of Substrate-Permissive Diketopiperazine Reverse Prenyltransferase NotF: Applications in Protein Engineering and Cascade Biocatalytic Synthesis of (−)-Eurotiumin A
Data Science-Driven Analysis of Substrate-Permissive Diketopiperazine Reverse Prenyltransferase NotF: Applications in Protein Engineering and Cascade Biocatalytic Synthesis of (−)-Eurotiumin A Open
Prenyltransfer is an early-stage carbon-hydrogen bond (C-H) functionalization prevalent in the biosynthesis of a diverse array of biologically active bacterial, fungal, plant, and metazoan diketopiperazine (DKP) alkaloids. Toward the devel…
View article: A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA
A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA Open
View article: The enzyme EryM gives a helping hand in natural product biosynthesis
The enzyme EryM gives a helping hand in natural product biosynthesis Open
Polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) are essential enzymes responsible for the assembly of natural products, such as the common antibiotic erythromycin and the siderophore erythrochelin.Most bacterial PKS…
View article: KHNYN is a zinc-finger antiviral protein (ZAP) co-factor that degrades ZAP-bound RNA
KHNYN is a zinc-finger antiviral protein (ZAP) co-factor that degrades ZAP-bound RNA Open
View article: Progress towards increasing automation and sample capacity at the GM/CA beamlines for the upgraded APS (APS-U)
Progress towards increasing automation and sample capacity at the GM/CA beamlines for the upgraded APS (APS-U) Open
The Advanced Photon Source (APS) is rapidly approaching its yearlong shutdown for the upgrade to the APS-U starting in April of 2023.The GM/CA beamlines at Sector 23 have begun to prepare for the higher brightness of the new source.Install…
View article: Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-Dependent <i>C</i>-Methyltransferases
Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-Dependent <i>C</i>-Methyltransferases Open
Installation of methyl groups can significantly improve the binding of small-molecule drugs to protein targets; however, site-selective methylation often presents a significant synthetic challenge. Metal- and S-adenosyl-methionine (…
View article: User capabilities at the GM/CA@APS Structural Biology Facility at the Advanced Photon Source
User capabilities at the GM/CA@APS Structural Biology Facility at the Advanced Photon Source Open