J. Bernard Heymann
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View article: The Triplex-Centric Assembly and Maturation of the Herpesvirus Procapsid
The Triplex-Centric Assembly and Maturation of the Herpesvirus Procapsid Open
Herpesviruses are prevalent infectious agents in humans, with complex structures and life cycles. The viability and detail of a model of capsid assembly and maturation can now be examined against the recently available mature herpesvirus c…
View article: The Triplex-Centric Assembly and Maturation of the Herpesvirus Procapsid
The Triplex-Centric Assembly and Maturation of the Herpesvirus Procapsid Open
Herpesviruses are prevalent infectious agents in humans, with complex structures and life cycles. The viability and detail of a model of capsid assembly and maturation can now be examined against the recently available mature herpesvirus c…
View article: Revisiting the symmetry-resolved entanglement for noninvertible symmetries in <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:mn>1</mml:mn><mml:mo>+</mml:mo><mml:mn>1</mml:mn><mml:mi mathvariant="normal">d</mml:mi></mml:mrow></mml:math> conformal field theories
Revisiting the symmetry-resolved entanglement for noninvertible symmetries in conformal field theories Open
Recently, a framework for computing the symmetry-resolved entanglement entropy for noninvertible symmetries in 1+1d conformal field theories has been proposed by Saura-Bastida []. We revisit their theoretical setup, paying particular atten…
View article: Community recommendations on cryoEM data archiving and validation
Community recommendations on cryoEM data archiving and validation Open
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data pr…
View article: Structural Studies of Bacteriophage Φ6 and Its Transformations during Its Life Cycle
Structural Studies of Bacteriophage Φ6 and Its Transformations during Its Life Cycle Open
From the first isolation of the cystovirus bacteriophage Φ6 from Pseudomonas syringae 50 years ago, we have progressed to a better understanding of the structure and transformations of many parts of the virion. The three-layered virion, en…
View article: Structural Studies on the Bacteriophage Φ6 and Its Transformations during Its Life Cycle
Structural Studies on the Bacteriophage Φ6 and Its Transformations during Its Life Cycle Open
From the first isolation of the cystovirus bacteriophage Φ6 from Pseudomonas syringae 50 years ago, we have progressed to a better understanding of the structure and transformations of the many parts of the virion. The three-layered virion…
View article: Accounting for the Ewald Spheres in CryoEM Reconstructions and Their Relationship to 3D Fourier Transforms of Focal Series
Accounting for the Ewald Spheres in CryoEM Reconstructions and Their Relationship to 3D Fourier Transforms of Focal Series Open
Journal Article Accounting for the Ewald Spheres in CryoEM Reconstructions and Their Relationship to 3D Fourier Transforms of Focal Series Get access J Bernard Heymann, J Bernard Heymann National Cryo-EM Program, Cancer Research Technology…
View article: Bsoft: Image Processing for Structural Biology
Bsoft: Image Processing for Structural Biology Open
Bsoft is a software package primarily developed for processing electron micrographs, with the goal of determining the structures of biologically relevant molecules, molecular assemblies, and parts of cells. However, it incorporates many wa…
View article: Cryo-Electron Tomography of the Herpesvirus Procapsid Reveals Interactions of the Portal with the Scaffold and a Shift on Maturation
Cryo-Electron Tomography of the Herpesvirus Procapsid Reveals Interactions of the Portal with the Scaffold and a Shift on Maturation Open
Herpes simplex virus 1 (HSV-1) infects a majority of humans, causing mostly mild disease but in some cases progressing toward life-threatening encephalitis. Understanding the life cycle of the virus is important to devise countermeasures.
View article: Cryo-electron tomography of the herpesvirus procapsid reveals interactions of the portal with the scaffold and a shift on maturation
Cryo-electron tomography of the herpesvirus procapsid reveals interactions of the portal with the scaffold and a shift on maturation Open
Herpes simplex virus type 1 (HSV-1) requires seven proteins to package its genome through a vertex in its capsid, one of which is the portal protein, pUL6. The portal protein is also thought to facilitate assembly of the procapsid. While t…
View article: High resolution electron tomography and segmentation‐by‐modeling interpretation in <scp>Bsoft</scp>
High resolution electron tomography and segmentation‐by‐modeling interpretation in <span>Bsoft</span> Open
Bsoft offers many tools for the processing of tomographic tilt series and the interpretation of tomograms. Since I introduced tomography into Bsoft almost two decades ago, the field has advanced significantly, requiring refinement of old a…
View article: The Mottled Capsid of the Salmonella Giant Phage SPN3US, a Likely Maturation Intermediate with a Novel Internal Shell
The Mottled Capsid of the Salmonella Giant Phage SPN3US, a Likely Maturation Intermediate with a Novel Internal Shell Open
“Giant” phages have genomes of >200 kbp, confined in correspondingly large capsids whose assembly and maturation are still poorly understood. Nevertheless, the first assembly product is likely to be, as in other tailed phages, a procapsid …
View article: FSC-Q: A CryoEM map-to-atomic model quality validation based on the local Fourier Shell Correlation
FSC-Q: A CryoEM map-to-atomic model quality validation based on the local Fourier Shell Correlation Open
In recent years, advances in cryoEM have dramatically increased the resolution of Coulomb potential maps and, with it, the number of solved atomic models. It is widely accepted that the quality of cryoEM maps varies locally; therefore, the…
View article: Protocols for Processing and Interpreting cryoEM Data Using Bsoft: A Case Study of the Retinal Adhesion Protein, Retinoschisin
Protocols for Processing and Interpreting cryoEM Data Using Bsoft: A Case Study of the Retinal Adhesion Protein, Retinoschisin Open
The goal of cryoEM is to determine the structures of biomolecules from electron micrographs. In many cases the processing is straightforward and can be handled with routine protocols. In other cases, the properties and behavior of the spec…
View article: Localization of the Herpesvirus Portal
Localization of the Herpesvirus Portal Open
Herpes simplex virus type 1 (HSV-1) contains a large double-stranded DNA genome of about 152,000 base pairs, which requires accommodation in a correspondingly massive particle.In the nucleus, viral DNA is packaged into a spherical precurso…
View article: Cryo-EM of retinoschisin branched networks suggests an intercellular adhesive scaffold in the retina
Cryo-EM of retinoschisin branched networks suggests an intercellular adhesive scaffold in the retina Open
Mutations in the retinal protein retinoschisin (RS1) cause progressive loss of vision in young males, a form of macular degeneration called X-linked retinoschisis (XLRS). We previously solved the structure of RS1, a 16-mer composed of pair…
View article: Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM
Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM Open
In single-particle analysis (SPA), the aim is to obtain a 3D reconstruction of a biological molecule from 2D electron micrographs to the highest level of detail or resolution as possible. Current practice is to collect large volumes of dat…
View article: Using Reconstruction Statistics to Predict the Number of Images Required for Single Particle Analysis
Using Reconstruction Statistics to Predict the Number of Images Required for Single Particle Analysis Open
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