Jared C. Lewis
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View article: Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof
Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof Open
Phosphorylation plays important roles in biology by modulating the structure, reactivity, and biological function of a broad range of molecules. Biocatalytic phosphorylation has attracted attention from synthetic chemists due to its select…
View article: Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof
Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof Open
Phosphorylation plays important roles in biology by modulating the structure, reactivity, and biological function of a broad range of molecules. Biocatalytic phosphorylation has attracted attention from synthetic chemists due to its select…
View article: Metabolic engineering and late-stage functionalization expand the chemical space of the antimalarial premarineosin A
Metabolic engineering and late-stage functionalization expand the chemical space of the antimalarial premarineosin A Open
Diversification of structurally complex natural products remains a key challenge in the discovery of next-generation therapeutics. Premarineosin A, a potent and selective antimalarial natural product, is a promising yet underexplored scaff…
View article: Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis
Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis Open
Organohalogen compounds are extensively used as building blocks, intermediates, pharmaceuticals, and agrochemicals due to their unique chemical and biological properties. Installing halogen substituents, however, frequently requires functi…
View article: Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis
Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis Open
Organohalogen compounds are extensively used as building blocks, intermediates, pharmaceuticals, and agrochemicals due to their unique chemical and biological properties. Installing halogen substituents, however, frequently requires functi…
View article: Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis
Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis Open
Organohalogen compounds are extensively used as building blocks, intermediates, pharmaceuticals, and agrochemicals due to their unique chemical and biological properties. Installing halogen substituents, however, frequently requires functi…
View article: Selective C−H Halogenation of Alkenes and Alkynes Using Flavin‐Dependent Halogenases
Selective C−H Halogenation of Alkenes and Alkynes Using Flavin‐Dependent Halogenases Open
Single component flavin‐dependent halogenases (FDHs) possess both flavin reductase and FDH activity in a single enzyme. We recently reported that the single component FDH AetF catalyzes site‐selective bromination and iodination of a variet…
View article: Selective C-H Halogenation of Alkenes and Alkynes Using Flavin-Dependent Halogenases
Selective C-H Halogenation of Alkenes and Alkynes Using Flavin-Dependent Halogenases Open
Single component flavin-dependent halogenases (FDHs) possesses both flavin reductase and FDH activity in a single enzyme. We recently reported that the single component FDH AetF catalyzes site-selective bromination and iodination of a vari…
View article: Non‐native Intramolecular Radical Cyclization Catalyzed by a B<sub>12</sub>‐Dependent Enzyme**
Non‐native Intramolecular Radical Cyclization Catalyzed by a B<sub>12</sub>‐Dependent Enzyme** Open
Despite the unique reactivity of vitamin B 12 and its derivatives, B 12 ‐dependent enzymes remain underutilized in biocatalysis. In this study, we repurposed the B 12 ‐dependent transcription factor CarH to enable non‐native radical cycliz…
View article: Non-native Radical Cyclization Catalyzed by a B12-Dependent Enzyme
Non-native Radical Cyclization Catalyzed by a B12-Dependent Enzyme Open
Despite the unique reactivity of vitamin B12 and its derivatives, B12-dependent enzymes remain underutilized in biocatalysis. In this study, we repurpose the B12-dependent transcription factor CarH to enable non-native radical cyclization …
View article: Non-Native Site-Selective Enzyme Catalysis
Non-Native Site-Selective Enzyme Catalysis Open
The ability to site-selectively modify equivalent functional groups in a molecule has the potential to streamline syntheses and increase product yields by lowering step counts. Enzymes catalyze site-selective transformations throughout pri…
View article: Deep learning for stochastic precipitation generation – deep SPG v1.0
Deep learning for stochastic precipitation generation – deep SPG v1.0 Open
We present a deep-neural-network-based single-site stochastic precipitation generator (SPG), capable of producing realistic time series of daily and hourly precipitation. The neural network outputs a wet-day probability and precipitation d…
View article: Non-Native Site-Selective Enzyme Catalysis
Non-Native Site-Selective Enzyme Catalysis Open
The ability to a site-selectively modify equivalent functional groups in a molecule has the potential to streamline syntheses and increase product yields by lowering step counts. Enzymes catalyze site-selective transformations throughout p…
View article: Iridium(III) Polypyridine Artificial Metalloenzymes with Tunable Photophysical Properties: a New Platform for Visible Light Photocatalysis in Aqueous Solution
Iridium(III) Polypyridine Artificial Metalloenzymes with Tunable Photophysical Properties: a New Platform for Visible Light Photocatalysis in Aqueous Solution Open
Visible light absorbing iridium(III) complexes have been widely employed as photocatalysts in organic synthesis. Their catalytic reactivity and selectivity are generally optimized by modifying the structures of coordinated ligands to obtai…
View article: Asymmetric catalysis by flavin‐dependent halogenases
Asymmetric catalysis by flavin‐dependent halogenases Open
In nature, flavin‐dependent halogenases (FDHs) catalyze site‐selective chlorination and bromination of aromatic natural products. This ability has led to extensive efforts to engineer FDHs for selective chlorination, bromination, and iodin…
View article: Directed Evolution of an Iron(II)‐ and α‐Ketoglutarate‐Dependent Dioxygenase for Site‐Selective Azidation of Unactivated Aliphatic C−H Bonds**
Directed Evolution of an Iron(II)‐ and α‐Ketoglutarate‐Dependent Dioxygenase for Site‐Selective Azidation of Unactivated Aliphatic C−H Bonds** Open
Fe II ‐ and α‐ketoglutarate‐dependent halogenases and oxygenases can catalyze site‐selective functionalization of C−H bonds via a variety of C−X bond forming reactions, but achieving high chemoselectivity for functionalization using non‐na…
View article: Directed Evolution of an Iron(II)‐ and α‐Ketoglutarate‐Dependent Dioxygenase for Site‐Selective Azidation of Unactivated Aliphatic C−H Bonds**
Directed Evolution of an Iron(II)‐ and α‐Ketoglutarate‐Dependent Dioxygenase for Site‐Selective Azidation of Unactivated Aliphatic C−H Bonds** Open
Fe II ‐ and α‐ketoglutarate‐dependent halogenases and oxygenases can catalyze site‐selective functionalization of C−H bonds via a variety of C−X bond forming reactions, but achieving high chemoselectivity for functionalization using non‐na…
View article: In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes
In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes Open
The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for pro…
View article: Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes
Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes Open
The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for pro…
View article: First and second sphere interactions accelerate non-native <i>N</i> -alkylation catalysis by the thermostable, methanol-tolerant B <sub>12</sub> -dependent enzyme MtaC
First and second sphere interactions accelerate non-native <i>N</i> -alkylation catalysis by the thermostable, methanol-tolerant B <sub>12</sub> -dependent enzyme MtaC Open
The corrinoid protein MtaC, which is natively involved in methyl transferase catalysis, catalyzes N -alkylation of aniline using ethyl diazoacetate.
View article: Iridium( <scp>iii</scp> ) polypyridine artificial metalloenzymes with tunable photophysical properties: a new platform for visible light photocatalysis in aqueous solution
Iridium( <span>iii</span> ) polypyridine artificial metalloenzymes with tunable photophysical properties: a new platform for visible light photocatalysis in aqueous solution Open
This study shows that Ir( iii )-based artificial metalloenzymes constitute a promising starting point for ongoing studies to control the stereoselectivity of visible-light-induced EnT reactions by engineering substrate binding/activation m…
View article: Asymmetric Catalysis by Flavin Dependent Halogenases
Asymmetric Catalysis by Flavin Dependent Halogenases Open
In nature, flavin dependent halogenases (FDHs) catalyze site-selective chlorination and bromination of aromatic natural products. This ability has led to extensive efforts to engineer FDHs for selective chlorination, bromination, and iodin…
View article: Iridium(III) Polypyridine Artificial Metalloenzymes with Tunable Photophysical Properties: a New Platform for Visible Light Photocatalysis in Aqueous Solution
Iridium(III) Polypyridine Artificial Metalloenzymes with Tunable Photophysical Properties: a New Platform for Visible Light Photocatalysis in Aqueous Solution Open
Visible light absorbing iridium(III) complexes have been widely employed as photocatalysts in organic synthesis. Their catalytic reactivity and selectivity are generally optimized by modifying the structures of coordinated ligands to obtai…
View article: The Single‐Component Flavin Reductase/Flavin‐Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins**
The Single‐Component Flavin Reductase/Flavin‐Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins** Open
Flavin‐dependent halogenases (FDHs) natively catalyze selective halogenation of electron rich aromatic and enolate groups. Nearly all FDHs reported to date require a separate flavin reductase to supply them with FADH 2 , which complicates …
View article: The Single‐Component Flavin Reductase/Flavin‐Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins**
The Single‐Component Flavin Reductase/Flavin‐Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins** Open
Flavin‐dependent halogenases (FDHs) natively catalyze selective halogenation of electron rich aromatic and enolate groups. Nearly all FDHs reported to date require a separate flavin reductase to supply them with FADH 2 , which complicates …
View article: Expanding the Reactivity of Flavin-Dependent Halogenases toward Olefins via Enantioselective Intramolecular Haloetherification and Chemoenzymatic Oxidative Rearrangements
Expanding the Reactivity of Flavin-Dependent Halogenases toward Olefins via Enantioselective Intramolecular Haloetherification and Chemoenzymatic Oxidative Rearrangements Open
Of the different classes of halogenases characterized to date, flavin dependent halogenases (FDHs) are most associated with site-selective halogenation of electron rich arenes and enol(ate) moieties in the biosynthesis of halogenated natur…
View article: Expanding the Reactivity of Flavin Dependent Halogenases Toward Olefins via Enantioselective Intramolecular Haloetherification and Chemoenzymatic Oxidative Rearrangements
Expanding the Reactivity of Flavin Dependent Halogenases Toward Olefins via Enantioselective Intramolecular Haloetherification and Chemoenzymatic Oxidative Rearrangements Open
Of the different classes of halogenases characterized to date, flavin dependent halogenases (FDHs) are most commonly associated with site-selective halogenation of electron rich arenes and enol(ate) moieties in the biosynthesis of halogena…
View article: The Single Component Flavin Reductase/Flavin Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins
The Single Component Flavin Reductase/Flavin Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins Open
Flavin-dependent halogenases (FDHs) natively catalyze selective halogenation of electron rich aromatic and enolate groups. Nearly all FDHs reported to date require a separate flavin reductase to supply the FADH2¬ required by these enzymes.…
View article: Changes in IPCC scenario assessment emulators between SR1.5 and AR6 unravelled
Changes in IPCC scenario assessment emulators between SR1.5 and AR6 unravelled Open
The IPCC’s scientific assessment of the timing of net-zero emissions and 2030 emission reduction targets consistent with limiting warming to 1.5C or 2C rests on large scenario databases. Updates to this assessment, such as between the IPCC…
View article: Comment on gmd-2022-163
Comment on gmd-2022-163 Open
Abstract. We present a deep neural network based single site stochastic precipitation generator (SPG), capable of producing realistic time series of daily and hourly precipitation. The neural network outputs a wet day prob…