Jason C. Andrechak
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View article: Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia
Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia Open
Macrophages often pervade solid tumors, and clusters of macrophages sometimes associate with longer survival of patients. However, clustering mechanisms and impacts on key functions such as phagocytosis remain obscure. Here, under conditio…
View article: Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia
Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia Open
Macrophages often pervade solid tumors, but their nearest neighbor organization is understudied and potentially enables key functions such as phagocytosis. Here, we observe dynamic macrophage clusters in tumors under conditions that maximi…
View article: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG
Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG Open
Solid tumors generally exhibit chromosome copy number variation, which is typically caused by chromosomal instability (CIN) in mitosis. The resulting aneuploidy can drive evolution and associates with poor prognosis in various cancer types…
View article: Author response: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG
Author response: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG Open
View article: Author Response: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Author Response: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Titrating CD47 by mismatch CRISPR-interference reveals incomplete repression can eliminate IgG-opsonized tumors but limits induction of antitumor IgG
Titrating CD47 by mismatch CRISPR-interference reveals incomplete repression can eliminate IgG-opsonized tumors but limits induction of antitumor IgG Open
Phagocytic elimination of solid tumors by innate immune cells seems attractive for immunotherapy, particularly because of the possibilities for acquired immunity. However, the approach remains challenging, with blockade of the macrophage c…
View article: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG
Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG Open
Solid tumors generally exhibit chromosome copy number variation, which is typically caused by chromosomal instability (CIN) in mitosis. The resulting aneuploidy can drive evolution and associates with poor prognosis in various cancer types…
View article: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters
Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters Open
The mechanical environment of a cell can have many effects, but whether it impacts the DNA sequence of a cell has remained unexamined. To investigate this, we developed a live-cell method to measure changes in chromosome numbers. We edited…
View article: Cooperative phagocytosis of solid tumours by macrophages triggers durable anti-tumour responses
Cooperative phagocytosis of solid tumours by macrophages triggers durable anti-tumour responses Open
View article: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Confinement with Myosin-II suppression increases heritable loss of chromosomes, using live-cell ChReporters
Confinement with Myosin-II suppression increases heritable loss of chromosomes, using live-cell ChReporters Open
Matrix around cells exerts many effects, some of which depend on the putative tumor suppressor Myosin-II, but whether such factors affect DNA sequences in a cell remains unclear. Here, live-cell monitoring of changes to chromosome copy num…
View article: Titrating CD47 by mismatch CRISPRi reveals incomplete repression can eliminate IgG-opsonized tumors but CD47 heterogeneity limits induction of anti-tumor IgG
Titrating CD47 by mismatch CRISPRi reveals incomplete repression can eliminate IgG-opsonized tumors but CD47 heterogeneity limits induction of anti-tumor IgG Open
Phagocytic elimination of solid tumors is an attractive mechanism for immunotherapy – particularly because of the possible induction of anti-cancer immunity. The phagocytic potential of macrophages is limited, however, by the CD47-SIRPα ch…
View article: CD47-SIRPα Checkpoint Disruption in Metastases Requires Tumor-Targeting Antibody for Molecular and Engineered Macrophage Therapies
CD47-SIRPα Checkpoint Disruption in Metastases Requires Tumor-Targeting Antibody for Molecular and Engineered Macrophage Therapies Open
The macrophage checkpoint interaction CD47-SIRPα is an emerging target for cancer therapy, but clinical trials of monoclonal anti-CD47 show efficacy only in liquid tumors when combined with tumor-opsonizing IgG. Here, in challenging metast…
View article: Human CD47-Derived Cyclic Peptides Enhance Engulfment of mAb-Targeted Melanoma by Primary Macrophages
Human CD47-Derived Cyclic Peptides Enhance Engulfment of mAb-Targeted Melanoma by Primary Macrophages Open
CD47 on healthy cells, cancer cells, and even engineered particles can inhibit phagocytic clearance by binding SIRPα on macrophages. To mimic and modulate this interaction with peptides that could be used as soluble antagonists or potentia…
View article: Cooperative phagocytosis underlies macrophage immunotherapy of solid tumours and initiates a broad anti-tumour IgG response
Cooperative phagocytosis underlies macrophage immunotherapy of solid tumours and initiates a broad anti-tumour IgG response Open
Macrophages are abundant in solid tumours and typically associate with poor prognosis, but macrophage clusters in tumour nests have also been reported as beneficial even though dispersed macrophages would have more contacts with cancer cel…
View article: CD47-SIRPα checkpoint disruption in metastases requires tumor-targeting antibody for molecular and engineered macrophage therapies
CD47-SIRPα checkpoint disruption in metastases requires tumor-targeting antibody for molecular and engineered macrophage therapies Open
Data for the Cancers (2022) publication: CD47-SIRPα checkpoint disruption in metastases requires tumor-targeting antibody for molecular and engineered macrophage therapies
View article: Heterogeneous strains in tissue collagen show that high strains locally suppress degradation by collagenase
Heterogeneous strains in tissue collagen show that high strains locally suppress degradation by collagenase Open
Collagen, the most abundant protein in mammals, contributes to the physical properties of different tissues during development, homeostasis, and disease. The adaptation of physical properties of tissues to mechanical stimuli is thus depend…
View article: Live Cell Chromosome Loss Reporter Shows Mechanical Confinement Associates with Chromosome Mis-Segregation
Live Cell Chromosome Loss Reporter Shows Mechanical Confinement Associates with Chromosome Mis-Segregation Open
View article: Heterogeneously Strained Tissue Collagen Resists Collagenase Degradation where Strains are High
Heterogeneously Strained Tissue Collagen Resists Collagenase Degradation where Strains are High Open
View article: Solid Tumor Cures by Complete Disruption of the CD47:SIRPα Macrophage Checkpoint, Tumor Opsonization, and ‘Phagocytic Feedback’
Solid Tumor Cures by Complete Disruption of the CD47:SIRPα Macrophage Checkpoint, Tumor Opsonization, and ‘Phagocytic Feedback’ Open
View article: Multivalent, Soluble Nano-Self Peptides Increase Phagocytosis of Antibody-Opsonized Targets while Suppressing “Self” Signaling
Multivalent, Soluble Nano-Self Peptides Increase Phagocytosis of Antibody-Opsonized Targets while Suppressing “Self” Signaling Open
Macrophages engulf "foreign" cells and particles, but phagocytosis of healthy cells and cancer cells is inhibited by expression of the ubiquitous membrane protein CD47 which binds SIRPα on macrophages to signal "self". Motivated by some cl…
View article: Macrophage checkpoint blockade: results from initial clinical trials, binding analyses, and CD47-SIRPα structure–function
Macrophage checkpoint blockade: results from initial clinical trials, binding analyses, and CD47-SIRPα structure–function Open
The macrophage checkpoint is an anti-phagocytic interaction between signal regulatory protein alpha (SIRPα) on a macrophage and CD47 on all types of cells – ranging from blood cells to cancer cells. This interaction has emerged over the la…
View article: Mouse Melanoma B16 Tumors are Soft and Engulfable when Targeted in Combination with Macrophage Checkpoint Blockade
Mouse Melanoma B16 Tumors are Soft and Engulfable when Targeted in Combination with Macrophage Checkpoint Blockade Open
View article: Live Cell Monitoring of Chromosome Loss Reporter
Live Cell Monitoring of Chromosome Loss Reporter Open
View article: The macrophage checkpoint CD47 : SIRPα for recognition of ‘self’ cells: from clinical trials of blocking antibodies to mechanobiological fundamentals
The macrophage checkpoint CD47 : SIRPα for recognition of ‘self’ cells: from clinical trials of blocking antibodies to mechanobiological fundamentals Open
Immunotherapies against some solid tumour types have recently shown unprecedented, durable cures in the clinic, and the most successful thus far involves blocking inhibitory receptor ‘checkpoints’ on T cells. A similar approach with macrop…
View article: Macrophage Checkpoint Blockade and Tumor Mechanics in a Cell-Based Immunotherapy
Macrophage Checkpoint Blockade and Tumor Mechanics in a Cell-Based Immunotherapy Open
View article: Supplementary material from "The macrophage checkpoint CD47:SIRP<i>α</i> for recognition of ‘self’ cells: from clinical trials of blocking antibodies to mechanobiological fundamentals"
Supplementary material from "The macrophage checkpoint CD47:SIRP<i>α</i> for recognition of ‘self’ cells: from clinical trials of blocking antibodies to mechanobiological fundamentals" Open
Immunotherapies against some solid tumour types have recently shown unprecedented, durable cures in the clinic, and the most successful thus far involves blocking inhibitory receptor ‘checkpoints’ on T cells. A similar approach with macrop…
View article: Live cell monitoring for factors affecting genome variation
Live cell monitoring for factors affecting genome variation Open
Cancer cells and pluripotent stem cells frequently exhibit gains or losses of entire chromosomes and chromosome segments, and the typical terminal analyses of genomes suggest this aneuploidy is ongoing and particularly variable in solid tu…