Jason C. Rogalski
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View article: Circulating enolase 1 as a diagnostic biomarker for early-stage breast cancer
Circulating enolase 1 as a diagnostic biomarker for early-stage breast cancer Open
Diagnosis of stage 1 breast cancer is challenging as small tumors are often left undetected by conventional imaging techniques. In addition, ~80% of detected breast masses are classified as benign, which means that a large proportion of di…
View article: Omics Insights Into the Effects of Highbush Blueberry and Cranberry Crop Agroecosystems on Honey Bee Health and Physiology
Omics Insights Into the Effects of Highbush Blueberry and Cranberry Crop Agroecosystems on Honey Bee Health and Physiology Open
Honey bees ( Apis mellifera ) are vital pollinators in fruit‐producing agroecosystems like highbush blueberry (HBB) and cranberry (CRA). However, their health is threatened by multiple interacting stressors, including pesticides, pathogens…
View article: Reverse Proteolysis Uncovers a Hidden Dimension of the Peptidome
Reverse Proteolysis Uncovers a Hidden Dimension of the Peptidome Open
Proteases are classically viewed as degradative enzymes, yet they can also catalyze reverse proteolysis - a process known for decades but rarely examined in disease contexts. Here, we show that cysteine cathepsins efficiently form multi-ge…
View article: Investigation on the Influence of Protein Corona and Platelet Adhesion on Storage Bag Surface on the Platelet Storage Lesion
Investigation on the Influence of Protein Corona and Platelet Adhesion on Storage Bag Surface on the Platelet Storage Lesion Open
Platelet transfusion is an indispensable therapy used in contexts ranging from hemorrhagic bleeding to chemotherapy. However, platelet shelf life and quality deteriorate during storage in a process termed the platelet storage lesion (PSL),…
View article: The X‐Linked Intellectual Disability Gene, <scp><i>ZDHHC9</i></scp>, Is Important for Oligodendrocyte Subtype Determination and Myelination
The X‐Linked Intellectual Disability Gene, <span><i>ZDHHC9</i></span>, Is Important for Oligodendrocyte Subtype Determination and Myelination Open
Two percent of patients with X‐linked intellectual disability (XLID) exhibit loss‐of‐function mutations in the enzyme, ZDHHC9. One of the main anatomical deficits observed in these patients is a decrease in corpus callosum volume and a con…
View article: Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes
Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes Open
Pyruvate dehydrogenase kinase (PDK) 1 is one of four isozymes that inhibit the oxidative decarboxylation of pyruvate to acetyl-CoA via pyruvate dehydrogenase. PDK activity is elevated in fasting or starvation conditions to conserve carbohy…
View article: Signal transduction pathways controlling Ins2 gene activity and beta cell state transitions
Signal transduction pathways controlling Ins2 gene activity and beta cell state transitions Open
View article: Optimizing imputation strategies for mass spectrometry-based proteomics considering intensity and missing value rates
Optimizing imputation strategies for mass spectrometry-based proteomics considering intensity and missing value rates Open
View article: Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes
Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes Open
Pyruvate dehydrogenase kinase (PDK) 1 is one of four isozymes that inhibit the oxidative decarboxylation of pyruvate to acetyl-CoA via pyruvate dehydrogenase. PDK activity is elevated in fasting or starvation conditions to conserve carbohy…
View article: Proteomic predictors of individualized nutrient-specific insulin secretion in health and disease
Proteomic predictors of individualized nutrient-specific insulin secretion in health and disease Open
View article: Beta-Hydroxybutyrate Promotes Basal Insulin Secretion While Decreasing Glucagon Secretion in Mouse and Human Islets
Beta-Hydroxybutyrate Promotes Basal Insulin Secretion While Decreasing Glucagon Secretion in Mouse and Human Islets Open
Dietary carbohydrates raise blood glucose levels, and limiting carbohydrate intake improves glycemia in patients with type 2 diabetes. Low carbohydrate intake (< 25 g) allows the body to utilize fat as its primary fuel. As a consequence…
View article: HumanIslets: An integrated platform for human islet data access and analysis
HumanIslets: An integrated platform for human islet data access and analysis Open
SUMMARY Comprehensive molecular and cellular phenotyping of human islets can enable deep mechanistic insights for diabetes research. We established the Human Islet Data Analysis and Sharing (HI-DAS) consortium to advance goals in accessibi…
View article: Signal transduction pathways controlling <i>Ins2</i> gene activity and beta cell state transitions
Signal transduction pathways controlling <i>Ins2</i> gene activity and beta cell state transitions Open
Summary Pancreatic β cells exist in low and high insulin gene activity states that are dynamic on a scale of hours to days. Here, we used live 3D imaging, mass spectrometry proteomics, and targeted perturbations of β cell signaling to comp…
View article: Beta-hydroxybutyrate promotes basal insulin secretion while decreasing glucagon secretion in mouse and human islets
Beta-hydroxybutyrate promotes basal insulin secretion while decreasing glucagon secretion in mouse and human islets Open
Dietary carbohydrates raise blood glucose and limiting carbohydrate intake improves glycemia in patients with type 2 diabetes. Low carbohydrate intake (< 25 g) allows the body to utilize fat as its primary fuel. As a consequence of increas…
View article: Methods to extract and analyze fluid from human pluripotent stem cell-derived choroid plexus organoids
Methods to extract and analyze fluid from human pluripotent stem cell-derived choroid plexus organoids Open
The choroid plexus (ChP) is a highly vascularized tissue lining the ventricular space of the brain. The ChP generates cerebrospinal fluid (CSF) and forms a protective barrier in the central nervous system (CNS). Recently, a three-dimension…
View article: The X-Linked Intellectual Disability gene,<i>ZDHHC9</i>, is important for oligodendrocyte subtype determination and myelination
The X-Linked Intellectual Disability gene,<i>ZDHHC9</i>, is important for oligodendrocyte subtype determination and myelination Open
Two percent of patients with X-linked intellectual disability (XLID) exhibit loss-of-function mutations in the enzyme, ZDHHC9 . One of the main anatomical deficits observed in these patients is a decrease in corpus callosum volume and a co…
View article: Proteomic predictors of individualized nutrient-specific insulin secretion in health and disease
Proteomic predictors of individualized nutrient-specific insulin secretion in health and disease Open
Population level variation and molecular mechanisms behind insulin secretion in response to carbohydrate, protein, and fat remain uncharacterized despite ramifications for personalized nutrition. Here, we define prototypical insulin secret…
View article: Bicyclic Picomolar OGA Inhibitors Enable Chemoproteomic Mapping of Its Endogenous Post-translational Modifications
Bicyclic Picomolar OGA Inhibitors Enable Chemoproteomic Mapping of Its Endogenous Post-translational Modifications Open
Owing to its roles in human health and disease, the modification of nuclear, cytoplasmic, and mitochondrial proteins with O-linked N-acetylglucosamine residues (O-GlcNAc) has emerged as a topic of great interest. Despite the presenc…
View article: Insulin-dependent and -independent dynamics of insulin receptor trafficking in muscle cells
Insulin-dependent and -independent dynamics of insulin receptor trafficking in muscle cells Open
Insulin resistance contributes to type 2 diabetes and can be driven by hyperinsulinemia. Insulin receptor (INSR) internalization and cell-surface dynamics at rest and during insulin exposure are incompletely understood in muscle cells. Usi…
View article: Proteomic portraits reveal evolutionarily conserved and divergent responses to spinal cord injury
Proteomic portraits reveal evolutionarily conserved and divergent responses to spinal cord injury Open
Despite the emergence of promising therapeutic approaches in preclinical studies, the failure of large-scale clinical trials leaves clinicians without effective treatments for acute spinal cord injury (SCI). These trials are hindered by th…
View article: Proteomic Portraits Reveal Evolutionarily Conserved and Divergent Responses to Spinal Cord Injury
Proteomic Portraits Reveal Evolutionarily Conserved and Divergent Responses to Spinal Cord Injury Open
Despite the emergence of promising therapeutic approaches in preclinical studies, the failure of large-scale clinical trials leaves clinicians without effective treatments for acute spinal cord injury (SCI). These trials are hindered by th…
View article: Blood circulation of soft nanomaterials is governed by dynamic remodeling of protein opsonins at nano-biointerface
Blood circulation of soft nanomaterials is governed by dynamic remodeling of protein opsonins at nano-biointerface Open
View article: Phase separation and clustering of an ABC transporter in <i>Mycobacterium tuberculosis</i>
Phase separation and clustering of an ABC transporter in <i>Mycobacterium tuberculosis</i> Open
Significance Nanoclustering has emerged as an organization principle of membrane proteins. This form of compartmentalization has been observed in eukaryotes and, to a lesser extent, in bacteria. Bacterial membrane proteins in secretion sys…
View article: The long noncoding <scp>RNA </scp><i><scp>HORAS</scp>5</i> mediates castration‐resistant prostate cancer survival by activating the androgen receptor transcriptional program
The long noncoding <span>RNA </span><i><span>HORAS</span>5</i> mediates castration‐resistant prostate cancer survival by activating the androgen receptor transcriptional program Open
Prostate cancer ( PC a) is driven by the androgen receptor ( AR )‐signaling axis. Hormonal therapy often mitigates PC a progression, but a notable number of cases progress to castration‐resistant PC a ( CRPC ). CRPC retains AR activity and…
View article: Catabolism of the Last Two Steroid Rings in <i>Mycobacterium tuberculosis</i> and Other Bacteria
Catabolism of the Last Two Steroid Rings in <i>Mycobacterium tuberculosis</i> and Other Bacteria Open
Most mycolic acid-containing actinobacteria and some proteobacteria use steroids as growth substrates, but the catabolism of the last two steroid rings has yet to be elucidated. In Mycobacterium tuberculosis , this pathway includes virulen…
View article: Identification and Characterization of Arabidopsis Seed Coat Mucilage Proteins
Identification and Characterization of Arabidopsis Seed Coat Mucilage Proteins Open
Plant cell wall proteins are important regulators of cell wall architecture and function. However, because cell wall proteins are difficult to extract and analyze, they are generally poorly understood. Here, we describe the identification …
View article: Identification of Avian Corticosteroid-binding Globulin (SerpinA6) Reveals the Molecular Basis of Evolutionary Adaptations in SerpinA6 Structure and Function as a Steroid-binding Protein
Identification of Avian Corticosteroid-binding Globulin (SerpinA6) Reveals the Molecular Basis of Evolutionary Adaptations in SerpinA6 Structure and Function as a Steroid-binding Protein Open
Corticosteroid-binding globulin (CBG) was isolated from chicken serum and identified by mass spectrometry and genomic analysis. This revealed that the organization and synteny of avian and mammalian SerpinA6 genes are conserved. Recombinan…