Jason R. Spence
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View article: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids
Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids Open
The role of FGF is the least understood of the morphogens driving mammalian gastrulation. Here we investigated the function of FGF in a stem cell model for human gastrulation known as a 2D gastruloid. We found a ring of FGF-dependent ERK a…
View article: Author response: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids
Author response: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids Open
View article: eLife Assessment: Cataloguing the postnatal small intestinal transcriptome during the first postnatal month
eLife Assessment: Cataloguing the postnatal small intestinal transcriptome during the first postnatal month Open
View article: Clinical Interventions and Inflammatory Signaling Shape the Transcriptional and Cellular Architecture of the Early Postnatal Lung
Clinical Interventions and Inflammatory Signaling Shape the Transcriptional and Cellular Architecture of the Early Postnatal Lung Open
The early postnatal period in human development is characterized by extensive remodeling of the distal lung to support gas exchange, but this critical period remains poorly understood. Here, we constructed a comprehensive cellular atlas of…
View article: Maternal Smoking and CC-16: Implications for Lung Development and COPD Across the Lifespan
Maternal Smoking and CC-16: Implications for Lung Development and COPD Across the Lifespan Open
Maternal smoking was linked to reduced circulating and airway CC16 in COPD patients, controls, and a preclinical murine COPD model. In human adults, lower CC16 correlated with accelerated lung function decline and emphysema progression, wh…
View article: Dynamics of YAP localization and transcript activity in human oocytes and granulosa cells across early-stage folliculogenesis: an exploratory investigation
Dynamics of YAP localization and transcript activity in human oocytes and granulosa cells across early-stage folliculogenesis: an exploratory investigation Open
Purpose The transcription factor yes-associated protein (YAP) has been implicated in the regulation of murine follicle activation at the level of granulosa cells (GCs); however, less is known about its role in human folliculogenesis. This …
View article: Human-mouse cross-species comparison identifies common and unique aspects of intestinal mesenchyme development
Human-mouse cross-species comparison identifies common and unique aspects of intestinal mesenchyme development Open
Summary Using single-cell RNA sequencing (scRNA-seq) and histological approaches, we examine cross-species cellular identity, diversity and organization of mesenchymal (fibroblast) populations in the developing mouse and human intestines. …
View article: Mapping mesenchymal diversity in the developing human intestine and organoids
Mapping mesenchymal diversity in the developing human intestine and organoids Open
Summary The organization of diverse mesenchymal populations during human intestinal development is critical for tissue architecture and function yet remains poorly defined. To construct a comprehensive, tissue-scale map of the developing h…
View article: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems
Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems Open
Apicobasal polarization is crucial for tissue organization during in vivo development and in human organoid models. Extracellular matrix (ECM) signaling typically provides a basal cue, and intestinal and lung organoids reverse polarity fro…
View article: An integrated transcriptomic cell atlas of human endoderm-derived organoids
An integrated transcriptomic cell atlas of human endoderm-derived organoids Open
View article: SOX9 suppresses colon cancer via inhibiting epithelial-mesenchymal transition and SOX2 induction
SOX9 suppresses colon cancer via inhibiting epithelial-mesenchymal transition and SOX2 induction Open
The Wnt/β-catenin pathway regulates expression of the SOX9 gene, which encodes sex-determining region Y-box (SOX) transcription factor 9, a differentiation factor and potential β-catenin regulator. Because APC tumor suppressor defects in a…
View article: Strain- and vaccine-specific effects of serum antibodies in the protection of intestinal SARS-CoV-2 infection
Strain- and vaccine-specific effects of serum antibodies in the protection of intestinal SARS-CoV-2 infection Open
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains a public health challenge worldwide. The gastrointestinal tract has emerged as an important site of infection and has been implicated as a reservoir …
View article: Dynamic Reprogramming of Stromal Pdgfra-expressing cells during WNT-Mediated Transformation of the Intestinal Epithelium
Dynamic Reprogramming of Stromal Pdgfra-expressing cells during WNT-Mediated Transformation of the Intestinal Epithelium Open
Stromal fibroblasts regulate critical signaling gradients along the intestinal crypt-villus axis 1 and provide a niche that supports adjacent epithelial stem cells. Here we report that Pdgfra -expressing fibroblasts secrete ligands that pr…
View article: Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels
Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels Open
Human induced pluripotent stem cell (iPSC)-derived alveolar organoids have emerged as a system to model the alveolar epithelium in homeostasis and disease. However, alveolar organoids are typically grown in Matrigel, a mouse sarcoma-derive…
View article: Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation
Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation Open
Bronchospheres have emerged as a promising in vitro model toward probing questions on organ development and disease. Several organoid models, including from airway (e.g., bronchial, tracheal) cells, require three‐dimensional (3D) Matrigel,…
View article: Neutrophil prime unique transcriptional responses in intestinal organoids during infection with nontyphoidal <i>Salmonella enterica</i> serovars
Neutrophil prime unique transcriptional responses in intestinal organoids during infection with nontyphoidal <i>Salmonella enterica</i> serovars Open
Nontyphoidal strains of Salmonella enterica are a major cause of foodborne illnesses, and infection with these bacteria results in inflammatory gastroenteritis. Polymorphonuclear leukocytes (PMNs), also known as neutrophils, are a dominant…
View article: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids
Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids Open
The role of FGF is the least understood of the morphogens driving mammalian gastrulation. Here we investigated the function of FGF in a stem cell model for human gastrulation known as a 2D gastruloid. We found a ring of FGF-dependent ERK a…
View article: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids
Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids Open
The role of FGF is the least understood of the morphogens driving mammalian gastrulation. Here we investigated the function of FGF in a stem cell model for human gastrulation known as a 2D gastruloid. We found a ring of FGF-dependent ERK a…
View article: Author response: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids
Author response: Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids Open
View article: In situ neutralization of the genotoxicity of pathogenic gut bacteria with the polyketide synthase island
In situ neutralization of the genotoxicity of pathogenic gut bacteria with the polyketide synthase island Open
The gut microbiota produce beneficial and deleterious metabolites to influence host physiology, with colibactin from pks+ bacteria one of the most studied metabolites associated with host genotoxicity and tumorigenesis. However,…
View article: Data from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Data from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Frequent (>70%) TP53 mutations often promote its protein stabilization, driving esophageal adenocarcinoma (EAC) development linked to poor survival and therapy resistance. We previously reported that during Barrett’s esophagus progr…
View article: Supplementary Figure S2 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S2 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S2. Full-length GRAIL1 does not interact with DNAJA1-GFP.
View article: Supplementary Figure S5 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S5 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S5. Intracellular localization of Frag J and Frag J-Δ.
View article: Supplementary Figure S4 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S4 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S4. Frag J/Jm mediated loss of misfolded Fluc-DM is independent of mutant p53 status.
View article: Supplementary Figure S1 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S1 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S1. Purification of GRAIL1 C-terminal fragment.
View article: Supplementary Figure S7 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S7 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S7. Design and testing of scrambled peptides as negative controls.
View article: Supplementary Table S1 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Table S1 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Table S1. Frag J-Jm Interactors
View article: Supplementary Figure S6 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S6 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S6. Metabolic stability of Pep J.
View article: Supplementary Figure S3 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
Supplementary Figure S3 from GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Supplementary Figure S3. GRAIL1 is a glycosylated protein that undergoes processing.
View article: GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function
GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin Ligase-Independent, Chaperone Regulatory Function Open
Frequent (>70%) TP53 mutations often promote its protein stabilization, driving esophageal adenocarcinoma (EAC) development linked to poor survival and therapy resistance. We previously reported that during Barrett’s esophagus progressi…