Javier Menasalvas
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View article: Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production in <i>Pseudomonas putida</i>
Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production in <i>Pseudomonas putida</i> Open
Synthetic biology generates vast combinatorial designs, yet high-throughput analytical methods to screen them are poorly matched to interrogate this search space. We address this challenge by developing a biosensor-driven, growth-coupled s…
View article: RB-TnSeq elucidates dicarboxylic-acid-specific catabolism in β-proteobacteria for improved plastic monomer upcycling
RB-TnSeq elucidates dicarboxylic-acid-specific catabolism in β-proteobacteria for improved plastic monomer upcycling Open
Dicarboxylic acids are key components of many polymers and plastics, making them a target for both engineered microbial degradation and sustainable bioproduction. In this study, we generated a comprehensive data set of functional evidence …
View article: Biosensor-Driven Strain Engineering Reveals Key Cellular Processes for Maximizing Isoprenol Production in <i>Pseudomonas putida</i>
Biosensor-Driven Strain Engineering Reveals Key Cellular Processes for Maximizing Isoprenol Production in <i>Pseudomonas putida</i> Open
Synthetic biology tools have accelerated the generation of simple mutants, but combinatorial testing remains challenging. High-throughput methods struggle translating from proof-of-principle molecules to advanced bioproducts. We address th…
View article: RB-TnSeq elucidates dicarboxylic acid specific catabolism in β-proteobacteria for improved plastic monomer upcycling
RB-TnSeq elucidates dicarboxylic acid specific catabolism in β-proteobacteria for improved plastic monomer upcycling Open
Dicarboxylic acids are key components of many polymers and plastics, making them a target for both engineered microbial degradation and sustainable bioproduction. In this study, we generated a comprehensive dataset of functional evidence f…
View article: Addressing genome scale design tradeoffs in Pseudomonas putida for bioconversion of an aromatic carbon source
Addressing genome scale design tradeoffs in Pseudomonas putida for bioconversion of an aromatic carbon source Open
Genome-scale metabolic models (GSMM) are commonly used to identify gene deletion sets that result in growth coupling and pairing product formation with substrate utilization and can improve strain performance beyond levels typically access…
View article: Bottlenecks in the Implementation of Genome Scale Metabolic Model Based Designs for Bioproduction from Aromatic Carbon Sources
Bottlenecks in the Implementation of Genome Scale Metabolic Model Based Designs for Bioproduction from Aromatic Carbon Sources Open
Genome scale metabolic models (GSMM) are commonly used to identify gene deletion sets that result in growth coupling, pairing product formation with substrate utilization. While such approaches can improve strain performance beyond levels …
View article: Maximizing microbial bioproduction from sustainable carbon sources using iterative systems engineering
Maximizing microbial bioproduction from sustainable carbon sources using iterative systems engineering Open
Maximizing the production of heterologous biomolecules is a complex problem that can be addressed with a systems-level understanding of cellular metabolism and regulation. Specifically, growth-coupling approaches can increase product titer…
View article: Ensemble and Iterative Engineering for Maximized Bioconversion to the Blue Pigment, Indigoidine from Non-Canonical Sustainable Carbon Sources
Ensemble and Iterative Engineering for Maximized Bioconversion to the Blue Pigment, Indigoidine from Non-Canonical Sustainable Carbon Sources Open
While many heterologous molecules can be produced at trace concentrations via microbial bioconversion processes, maximizing their titers, rates, and yields from lignin-derived carbon streams remains challenging. Growth coupling can not onl…
View article: Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels
Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels Open
View article: Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels
Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels Open
Synechococcus elongatus UTEX 2973 can use light and CO 2 to produce sucrose, making them promising candidates to construct cocultures with heterotrophic workhorses. This envisioned process is, however, challenging to implement because of p…
View article: Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production
Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production Open
In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous indigoidine production was examined using three distinct omic datasets. Transcriptomic data indicated that CRISPR/dCpf1-interference (CRISPRi) mediate…
View article: Optimizing a High Performing Multiplex-CRISPRi <i>P. putida</i> Strain with Integrated Metabolomics and <sup>13</sup>C-Metabolic Flux Analyses
Optimizing a High Performing Multiplex-CRISPRi <i>P. putida</i> Strain with Integrated Metabolomics and <sup>13</sup>C-Metabolic Flux Analyses Open
Microbial cell factory development often faces bottlenecks after initial rounds of design-build-test-learn (DBTL) cycles as engineered producers respond unpredictably to further genetic modifications. Thus, deciphering metabolic flux and c…