J.D. Hillman
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View article: An Alternative Bactericidal Mechanism of Action for Lantibiotic Peptides That Target Lipid II
An Alternative Bactericidal Mechanism of Action for Lantibiotic Peptides That Target Lipid II Open
Lantibiotics are polycyclic peptides containing unusual amino acids, which have binding specificity for bacterial cells, targeting the bacterial cell wall component lipid II to form pores and thereby lyse the cells. Yet several members of …
View article: Genetic and Biochemical Analysis of Mutacin 1140, a Lantibiotic from <i>Streptococcus mutans</i>
Genetic and Biochemical Analysis of Mutacin 1140, a Lantibiotic from <i>Streptococcus mutans</i> Open
Streptococcus mutans JH1000 and its derivatives were previously shown (J. D. Hillman, K. P. Johnson, and B. I. Yaphe, Infect. Immun. 44:141–144, 1984) to produce a low-molecular-weight, broad-spectrum bacteriocin-like inhibitory substance …
View article: Cloning and characterization of a Prevotella melaninogenica hemolysin
Cloning and characterization of a Prevotella melaninogenica hemolysin Open
Hemolysins have been proven to be important virulence factors in many medically relevant pathogenic organisms. Their production has also been implicated in the etiology of periodontal disease. Hemolytic strain 361B of Prevotella melaninoge…
View article: Genetic and physiologic analysis of a formyl-tetrahydrofolate synthetase mutant of Streptococcus mutans
Genetic and physiologic analysis of a formyl-tetrahydrofolate synthetase mutant of Streptococcus mutans Open
Previously we reported that transposon Tn917 mutagenesis of Streptococcus mutans JH1005 yielded an isolate detective in its normal ability to produce a mutacin (P. J. Crowley, J. D. Hillman, and A. S. Bleiweis, abstr. D55, p. 258 in Abstra…
View article: The hemagglutinin genes hagB and hagC of Porphyromonas gingivalis are transcribed in vivo as shown by use of a new expression vector
The hemagglutinin genes hagB and hagC of Porphyromonas gingivalis are transcribed in vivo as shown by use of a new expression vector Open
The hemagglutinin genes hagB and hagC of Porphyromonas gingivalis, a putative periodontopathic microorganism, have been cloned, sequenced, and characterized. However, the roles of these putative virulence genes have not yet been determined…
View article: Genetic and physiological analysis of the lethal effect of L-(+)-lactate dehydrogenase deficiency in Streptococcus mutans: complementation by alcohol dehydrogenase from Zymomonas mobilis
Genetic and physiological analysis of the lethal effect of L-(+)-lactate dehydrogenase deficiency in Streptococcus mutans: complementation by alcohol dehydrogenase from Zymomonas mobilis Open
CH4ts is a previously isolated recombinant mutant of Streptococcus mutans NG8 which produces a thermolabile L-(+)-lactate dehydrogenase (LDH) activity. It does not grow at 42 degrees C under a variety of cultivation conditions. In this stu…
View article: Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements
Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements Open
New vectors were constructed for efficient transposon Tn917-mediated mutagenesis of poorly transformable strains of Streptococcus mutans(pTV1-OK) and subsequent recovery of interrupted genes in Escherichia coli (pT21delta2TetM). In this re…
View article: L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans
L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans Open
The previously cloned gene for L-(+)-lactate dehydrogenase (LDH) from Streptococcus mutans was mutagenized in vitro. An Escherichia coli transformant which expressed a thermolabile LDH activity was identified. The ldh(Ts) gene was introduc…
View article: Evidence that L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans
Evidence that L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans Open
In order to construct an effector strain for the replacement therapy of dental caries, we wished to combine the properties of low-level acid production and high-level colonization potential in a strain of Streptococcus mutans. To this end,…
View article: DNA sequence and in vitro mutagenesis of the gene encoding the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase of Streptococcus mutans
DNA sequence and in vitro mutagenesis of the gene encoding the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase of Streptococcus mutans Open
Previously, the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase gene of Streptococcus mutans JH1000 was cloned into Escherichia coli (J. D. Hillman, M. J. Duncan, and K. P. Stashenko, Infect. Immun. 58:1290-1295, 1990). In t…
View article: Cloning and expression of the gene encoding the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase of Streptococcus mutans
Cloning and expression of the gene encoding the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase of Streptococcus mutans Open
The fructose-1,6-diphosphate-dependent lactate dehydrogenase from Streptococcus mutans JH1000 was purified by a modification of published methods. The sequence of 27 amino-terminal amino acids was determined, which allowed us to construct …
View article: Adaptive Changes in a Strain of<i>Streptococcus mutans</i>during Colonisation of the Human Oral Cavity
Adaptive Changes in a Strain of<i>Streptococcus mutans</i>during Colonisation of the Human Oral Cavity Open
When a bacterial strain undergoes a change in environment, its ability to colonise a new niche is optimised over time by the process of natural selection. In this study, a readily identifiable strain of Streptococcus mutans was recovered f…
View article: Acetoin production by wild-type strains and a lactate dehydrogenase-deficient mutant of Streptococcus mutans
Acetoin production by wild-type strains and a lactate dehydrogenase-deficient mutant of Streptococcus mutans Open
Eleven different laboratory strains of Streptococcus mutans representing the various serogroups were found to produce an average of 6.0 +/- 4.8 mM acetoin when grown in glucose-containing medium under aerobic conditions. None of the strain…
View article: Isolation of a Streptococcus mutans strain producing a novel bacteriocin
Isolation of a Streptococcus mutans strain producing a novel bacteriocin Open
A strain of Streptococcus mutans has been isolated that produces a bacteriocin with novel properties. Its antibiotic spectrum includes 123 of 124 strains of S. mutans tested and a variety of other gram-positive microorganisms. Experiments …
View article: Lactate dehydrogenase mutants of Streptococcus mutans: isolation and preliminary characterization
Lactate dehydrogenase mutants of Streptococcus mutans: isolation and preliminary characterization Open
Mutants of Streptococcus mutans were isolated which lack the enzyme activity L (+)-lactate dehydrogenase. Reversion studies indicate that the genetic defects are in the structural gene for the enzyme. The mutants produce less titratable ac…
View article: Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli
Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli Open
We describe glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. The gene (gap) is at approximately 34 min, with the transductional order gap-fadD-eda. One gap mutant is temperature sensitive and has a heat-labile enzyme. …
View article: New phosphoglucose isomerase mutants of Escherichia coli
New phosphoglucose isomerase mutants of Escherichia coli Open
The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and …