Jeff Abramson
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View article: Insights into VDAC Gating: Room-Temperature X-ray Crystal Structure of mVDAC-1
Insights into VDAC Gating: Room-Temperature X-ray Crystal Structure of mVDAC-1 Open
The voltage-dependent anion channel (VDAC) is a crucial mitochondrial protein that facilitates ion and metabolite exchange between mitochondria and the cytosol. Initially characterized over three decades ago, the structure of VDAC-1 was re…
View article: Membrane potential accelerates sugar uptake by stabilizing the outward facing conformation of the Na/glucose symporter vSGLT
Membrane potential accelerates sugar uptake by stabilizing the outward facing conformation of the Na/glucose symporter vSGLT Open
Sodium-dependent glucose transporters (SGLTs) couple a downhill Na + ion gradient to actively transport sugars. Here, we investigate the impact of the membrane potential on vSGLT structure and function using sugar uptake assays, double ele…
View article: Biochemical characterization of a GDP-mannose transporter from Chaetomium thermophilum
Biochemical characterization of a GDP-mannose transporter from Chaetomium thermophilum Open
Nucleotide Sugar Transporters (NSTs) belong to the SLC35 family (human solute carrier) of membrane transport proteins and are crucial components of the glycosylation machinery. NSTs are localized in the ER and Golgi apparatus membranes, wh…
View article: Biochemical characterization of a GDP-mannose transporter from<i>Chaetomium thermophilum</i>
Biochemical characterization of a GDP-mannose transporter from<i>Chaetomium thermophilum</i> Open
Nucleotide Sugar Transporters (NSTs) belong to the SLC35 family (human solute carrier) of membrane transport proteins and are crucial components of the glycosylation machinery. NSTs are localized in the ER and Golgi apparatus membranes, wh…
View article: The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating
The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating Open
The voltage-dependent anion channel (VDAC) is a β-barrel channel of the mitochondrial outer membrane (MOM) that passively transports ions, metabolites, polypeptides, and single-stranded DNA. VDAC responds to a transmembrane potential by "g…
View article: Functional and structural analyses of novel Smith-Kingsmore Syndrome-Associated MTOR variants reveal potential new mechanisms and predictors of pathogenicity
Functional and structural analyses of novel Smith-Kingsmore Syndrome-Associated MTOR variants reveal potential new mechanisms and predictors of pathogenicity Open
Smith-Kingsmore syndrome (SKS) is a rare neurodevelopmental disorder characterized by macrocephaly/megalencephaly, developmental delay, intellectual disability, hypotonia, and seizures. It is caused by dominant missense mutations in MTOR .…
View article: Genotype–phenotype correlations and novel molecular insights into the DHX30-associated neurodevelopmental disorders
Genotype–phenotype correlations and novel molecular insights into the DHX30-associated neurodevelopmental disorders Open
View article: ABCB10 exports mitochondrial biliverdin, driving metabolic maladaptation in obesity
ABCB10 exports mitochondrial biliverdin, driving metabolic maladaptation in obesity Open
ABCB10 in the liver impairs insulin signaling and promotes hyperglycemia and steatosis.
View article: Function Trumps Form in Two Sugar Symporters, LacY and vSGLT
Function Trumps Form in Two Sugar Symporters, LacY and vSGLT Open
Active transport of sugars into bacteria occurs through symporters driven by ion gradients. LacY is the most well-studied proton sugar symporter, whereas vSGLT is the most characterized sodium sugar symporter. These are members of the majo…
View article: Additional file 2 of Genotype–phenotype correlations and novel molecular insights into the DHX30-associated neurodevelopmental disorders
Additional file 2 of Genotype–phenotype correlations and novel molecular insights into the DHX30-associated neurodevelopmental disorders Open
Additional file 2: Table S1. Summary on clinical features of individuals bearing pathogenic DHX30 variants.
View article: MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel
MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel Open
Significance Microcrystal electron diffraction (MicroED) is an electron cryo-microscopy (cryoEM) method for determining structures using submicron crystals. Until now, determining structures of membrane proteins by MicroED required that th…
View article: Genotype–phenotype correlations and novel molecular insights into the <i>DHX30</i>-associated neurodevelopmental disorders
Genotype–phenotype correlations and novel molecular insights into the <i>DHX30</i>-associated neurodevelopmental disorders Open
Background We aimed to define the clinical and mutational spectrum, and to provide novel molecular insights into DHX30 -associated neurodevelopmental disorder. Methods Clinical and genetic data from affected individuals were collected thro…
View article: MicroED structure of lipid-embedded mammalian mitochondrial voltage dependent anion channel
MicroED structure of lipid-embedded mammalian mitochondrial voltage dependent anion channel Open
A near-atomic resolution structure of the mouse voltage dependent anion channel (mVDAC) is determined by combining cryogenic focused ion-beam (FIB) milling and microcrystal electron diffraction (MicroED). The crystals were grown in a visco…
View article: Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts
Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts Open
Native mass spectrometry (MS) provides the capacity to monitor membrane protein complexes and noncovalent binding of ligands and lipids to membrane proteins. The charge states produced by native MS of membrane proteins often result in gas-…
View article: Solving the Gating Mechanism of the Mitochondrial β-Barrel Metabolite Channel VDAC
Solving the Gating Mechanism of the Mitochondrial β-Barrel Metabolite Channel VDAC Open
View article: Reduced Affinity of Mitochondrial VDAC3 for Cytosolic Proteins Reveals a Mechanism for VDAC Isoform-Specific Physiology
Reduced Affinity of Mitochondrial VDAC3 for Cytosolic Proteins Reveals a Mechanism for VDAC Isoform-Specific Physiology Open
View article: Protein structure reveals how a malaria parasite imports a wide range of sugars
Protein structure reveals how a malaria parasite imports a wide range of sugars Open
View article: A lower affinity to cytosolic proteins reveals VDAC3 isoform-specific role in mitochondrial biology
A lower affinity to cytosolic proteins reveals VDAC3 isoform-specific role in mitochondrial biology Open
Voltage-dependent anion channel (VDAC) is the major pathway for the transport of ions and metabolites across the mitochondrial outer membrane. Among the three known mammalian VDAC isoforms, VDAC3 is the least characterized, but unique func…
View article: Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts
Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts Open
Native mass spectrometry paired with ion mobility (IM-MS) provides the capacity to monitor the structure of protein complexes and simultaneously assess small molecule binding to the protein. Native IM-MS typically utilizes positive mode el…
View article: Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts
Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts Open
Native mass spectrometry paired with ion mobility (IM-MS) provides the capacity to monitor the structure of protein complexes and simultaneously assess small molecule binding to the protein. Native IM-MS typically utilizes positive mode el…
View article: Multiple neurosteroid and cholesterol binding sites in voltage-dependent anion channel-1 determined by photo-affinity labeling
Multiple neurosteroid and cholesterol binding sites in voltage-dependent anion channel-1 determined by photo-affinity labeling Open
View article: Human VDAC3 Forms VDAC1-Type Anionic Channels that are High-Conducting, Permeable to Metabolites, and Regulated by Cytosolic Proteins
Human VDAC3 Forms VDAC1-Type Anionic Channels that are High-Conducting, Permeable to Metabolites, and Regulated by Cytosolic Proteins Open
View article: VDAC1 Conformational Changes Investigated by High Pressure DEER
VDAC1 Conformational Changes Investigated by High Pressure DEER Open
View article: Assessing the role of residue E73 and lipid headgroup charge in VDAC1 voltage gating
Assessing the role of residue E73 and lipid headgroup charge in VDAC1 voltage gating Open
View article: Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances
Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances Open
Proteins possess a complex and dynamic structure, which is influenced by external signals and may change as they perform their biological functions. We present an optical approach, distance-encoding photoinduced electron transfer (DEPET), …
View article: Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site
Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site Open
View article: Conformational transitions of the sodium-dependent sugar transporter, vSGLT
Conformational transitions of the sodium-dependent sugar transporter, vSGLT Open
Significance Transporters isomerize between conformations to shuttle cargo across membranes, but the mechanism is not understood. Double electron–electron resonance measurements on the sodium-dependent sugar transporter (vSGLT) were used t…
View article: Assessing the Role of Residue E73 in VDAC1 Voltage Gating
Assessing the Role of Residue E73 in VDAC1 Voltage Gating Open
View article: Protonation State of Glutamate 73 Regulates the Formation of a Unique Dimeric Association of VDAC1
Protonation State of Glutamate 73 Regulates the Formation of a Unique Dimeric Association of VDAC1 Open
View article: Protonation state of glutamate 73 regulates the formation of a specific dimeric association of mVDAC1
Protonation state of glutamate 73 regulates the formation of a specific dimeric association of mVDAC1 Open
Significance Cellular acidification occurs in response to many cellular events, including ischemia and apoptosis. The voltage-dependent anion channel (VDAC) is the most abundant protein in the outer mitochondrial membrane and has been impl…