Jeffrey A. Stuart
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View article: Differences in inflammatory markers, mitochondrial function, and synaptic proteins in male and female Alzheimer's disease <i>post mortem</i> brains
Differences in inflammatory markers, mitochondrial function, and synaptic proteins in male and female Alzheimer's disease <i>post mortem</i> brains Open
INTRODUCTION Inflammation and mitochondrial impairments are suggested to underlie Alzheimer's disease (AD) development. This study examined whether metabolic, synaptic, and inflammatory markers in AD differed from non‐demented brains. METH…
View article: Abstract 2620 Culture in Physiological Oxygen and Plasma-Like Media Modulate Breast Cancer Cell Hallmarks
Abstract 2620 Culture in Physiological Oxygen and Plasma-Like Media Modulate Breast Cancer Cell Hallmarks Open
View article: Abstract 2590 Physiological Oxygen Tensions Affect Metabolism, Gene Expression, and Hypoxic Responses In Vitro
Abstract 2590 Physiological Oxygen Tensions Affect Metabolism, Gene Expression, and Hypoxic Responses In Vitro Open
View article: Creatine and low-dose lithium supplementation separately alter energy expenditure, body mass, and adipose metabolism for the promotion of thermogenesis
Creatine and low-dose lithium supplementation separately alter energy expenditure, body mass, and adipose metabolism for the promotion of thermogenesis Open
View article: Human Brain Microvascular Endothelial Cells Exposure to SARS-CoV-2 Leads to Inflammatory Activation through NF-κB Non-Canonical Pathway and Mitochondrial Remodeling
Human Brain Microvascular Endothelial Cells Exposure to SARS-CoV-2 Leads to Inflammatory Activation through NF-κB Non-Canonical Pathway and Mitochondrial Remodeling Open
Neurological effects of COVID-19 and long-COVID-19, as well as neuroinvasion by SARS-CoV-2, still pose several questions and are of both clinical and scientific relevance. We described the cellular and molecular effects of the human brain …
View article: Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific Manner
Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific Manner Open
Standard cell culture is routinely performed at supraphysiological oxygen levels (~18% O2). Conversely, O2 levels in most mammalian tissues range from 1–6% (physioxia). Such hyperoxic conditions in cell culture can alter reactive oxygen sp…
View article: Low-dose lithium supplementation promotes adipose tissue browning and sarco(endo)plasmic reticulum Ca2+ ATPase uncoupling in muscle
Low-dose lithium supplementation promotes adipose tissue browning and sarco(endo)plasmic reticulum Ca2+ ATPase uncoupling in muscle Open
View article: Supraphysiological Oxygen Levels in Mammalian Cell Culture: Current State and Future Perspectives
Supraphysiological Oxygen Levels in Mammalian Cell Culture: Current State and Future Perspectives Open
Most conventional incubators used in cell culture do not regulate O2 levels, making the headspace O2 concentration ~18%. In contrast, most human tissues are exposed to 2–6% O2 (physioxia) in vivo. Accumulating evidence has shown that such …
View article: Oxygen toxicity: cellular mechanisms in normobaric hyperoxia
Oxygen toxicity: cellular mechanisms in normobaric hyperoxia Open
View article: Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific Manner
Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific Manner Open
Standard cell culture is routinely performed at supraphysiological oxygen concentrations (~18% O2). Conversely, oxygen levels in most tissues range from 1%–6% (physioxia). Such hyperoxic conditions can alter reactive oxygen species (…
View article: SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling Open
Neurological effects of COVID-19 and long-COVID-19 as well as neuroinvasion by SARS-CoV-2 still pose several questions and are of both clinical and scientific relevance. We described the cellular and molecular effects of the human brain mi…
View article: SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling Open
Neurological effects of COVID-19 and long-COVID-19 as well as neuroinvasion by SARS-CoV-2 still pose several questions and are of both clinical and scientific relevance. We described the cellular and molecular effects of the human brain mi…
View article: Heterozygous SOD2 deletion selectively impairs SERCA function in the soleus of female mice
Heterozygous SOD2 deletion selectively impairs SERCA function in the soleus of female mice Open
The sarco(endo)plasmic reticulum Ca 2+ ATPase (SERCA) restores intracellular Ca 2+ ([Ca 2+ ] i ) to resting levels after muscle contraction, ultimately eliciting relaxation. SERCA pumps are highly susceptible to tyrosine (T)‐nitration, imp…
View article: An Inexpensive Incubator for Mammalian Cell Culture Capable of Regulating O2, CO2, and Temperature
An Inexpensive Incubator for Mammalian Cell Culture Capable of Regulating O2, CO2, and Temperature Open
Mammalian cell culture is widely used for discovery and development. Recently, increasing attention has been paid to the importance of maintaining physiologically-relevant conditions in cell culture. Although oxygen level is a particularly…
View article: Bioabsorbable metal zinc differentially affects mitochondria in vascular endothelial and smooth muscle cells
Bioabsorbable metal zinc differentially affects mitochondria in vascular endothelial and smooth muscle cells Open
Zinc is an essential trace element having various structural, catalytic and regulatory interactions with an estimated 3000 proteins. Zinc has drawn recent attention for its use, both as pure metal and alloyed, in arterial stents due to its…
View article: The Effect of Oxygen and Micronutrient Composition of Cell Growth Media on Cancer Cell Bioenergetics and Mitochondrial Networks
The Effect of Oxygen and Micronutrient Composition of Cell Growth Media on Cancer Cell Bioenergetics and Mitochondrial Networks Open
Cancer cell culture is routinely performed under superphysiologic O2 levels and in media such as Dulbecco’s Modified Eagle Medium (DMEM) with nutrient composition dissimilar to mammalian extracellular fluid. Recently developed cell culture…
View article: Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availability
Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availability Open
Cell culture conditions highly influence cell metabolism in vitro . This is relevant for preclinical assays, for which fibroblasts are an interesting cell model, with applications in regenerative medicine, diagnostics and therapeutic devel…
View article: In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design Open
View article: Zn2+-dependent suppression of vascular smooth muscle intimal hyperplasia from biodegradable zinc implants
Zn2+-dependent suppression of vascular smooth muscle intimal hyperplasia from biodegradable zinc implants Open
View article: Calmodulin-Binding Proteins in Muscle: A Minireview on Nuclear Receptor Interacting Protein, Neurogranin, and Growth-Associated Protein 43
Calmodulin-Binding Proteins in Muscle: A Minireview on Nuclear Receptor Interacting Protein, Neurogranin, and Growth-Associated Protein 43 Open
Calmodulin (CaM) is an important Ca2+-sensing protein with numerous downstream targets that are either CaM-dependant or CaM-regulated. In muscle, CaM-dependent proteins, which are critical regulators of dynamic Ca2+ handling and contractil…
View article: A Low-Therapeutic Dose of Lithium Inhibits GSK3 and Enhances Myoblast Fusion in C2C12 Cells
A Low-Therapeutic Dose of Lithium Inhibits GSK3 and Enhances Myoblast Fusion in C2C12 Cells Open
Glycogen synthase kinase 3 (GSK3) slows myogenic differentiation and myoblast fusion partly by inhibiting the Wnt/β-catenin signaling pathway. Lithium, a common medication for bipolar disorder, inhibits GSK3 via Mg+ competition and increas…
View article: <i>In vitro</i>prototyping and rapid optimization of biosynthetic enzymes for cellular design
<i>In vitro</i>prototyping and rapid optimization of biosynthetic enzymes for cellular design Open
Microbial cell factories offer an attractive approach for production of biobased products. Unfortunately, designing, building, and optimizing biosynthetic pathways remains a complex challenge, especially for industrially-relevant, non-mode…
View article: Hypoxio: a simple solution to preventing pericellular hypoxia in cell monolayers growing at physiological oxygen levels
Hypoxio: a simple solution to preventing pericellular hypoxia in cell monolayers growing at physiological oxygen levels Open
View article: EFFECTS OF LONG-TERM DIETARY ADMINISTRATION OF ER-β AGONIST DIARYLPROPIONITRILE ON OVARIECTOMIZED CD-1 MICE
EFFECTS OF LONG-TERM DIETARY ADMINISTRATION OF ER-β AGONIST DIARYLPROPIONITRILE ON OVARIECTOMIZED CD-1 MICE Open
Diarylpropionitrile (DPN) is an estrogen receptor-β specific agonist with neuroprotective and anti-cancer properties, including improved maintenance of cognitive function with advanced age, the suppression of anxiety-like behaviours, and t…
View article: Oxygen and Glucose Levels in Cell Culture Media Determine Resveratrol’s Effects on Growth, Hydrogen Peroxide Production, and Mitochondrial Dynamics
Oxygen and Glucose Levels in Cell Culture Media Determine Resveratrol’s Effects on Growth, Hydrogen Peroxide Production, and Mitochondrial Dynamics Open
Resveratrol is a plant-derived polyphenol that has been widely studied for its putative health promoting effects. Many of those studies have been conducted in cell culture, in supra-physiological levels of oxygen and glucose. Resveratrol i…
View article: Resveratrol integrates metabolic and growth effects in PC3 prostate cancer cells‑involvement of prolyl hydroxylase and hypoxia inducible factor‑1
Resveratrol integrates metabolic and growth effects in PC3 prostate cancer cells‑involvement of prolyl hydroxylase and hypoxia inducible factor‑1 Open
Resveratrol (RES) is a polyphenol produced by certain plant species that has been well studied due to its ability to slow the growth of cancer cells. In numerous cell types and tissues, RES has been demonstrated to promote mitochondrial bi…
View article: Effects of long-term dietary administration of estrogen receptor-beta agonist diarylpropionitrile on ovariectomized female ICR (CD-1) mice
Effects of long-term dietary administration of estrogen receptor-beta agonist diarylpropionitrile on ovariectomized female ICR (CD-1) mice Open
View article: Microwave-assisted delivery of an anticancer drug to cancer cells
Microwave-assisted delivery of an anticancer drug to cancer cells Open
Exposure of MCF-7 breast and PC-3 prostate cancer cells to 10 W microwaves at 2.45 GHz increased their uptake of doxorubicin from media by almost 100%, concomitantly increasing cell death, while microwave exposure alone had no cellular tox…
View article: How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen‐Consuming Reactions
How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen‐Consuming Reactions Open
Most mammalian tissue cells experience oxygen partial pressures in vivo equivalent to 1–6% O 2 (i.e., physioxia). In standard cell culture, however, headspace O 2 levels are usually not actively regulated and under these conditions are ~18…