Jeffrey J. Bajramović
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View article: Finding a Place for New Approach Methodologies (NAMs) in Biomedical Research
Finding a Place for New Approach Methodologies (NAMs) in Biomedical Research Open
New Approach Methodologies (NAMs) can greatly contribute to the Replacement and Reduction of animal experiments in biomedical science. However, while NAMs have seen considerable investment and adoption in toxicology, this growth has not fo…
View article: Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media
Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media Open
Organoid technology can revolutionize biomedical research by increasing the translational value of experimental results while at the same time reducing the need for experimental animal use. However, in most cases the organoid culture workf…
View article: Developing a global education hub for animal-free innovation
Developing a global education hub for animal-free innovation Open
View article: Toward a common interpretation of the 3Rs principles in animal research
Toward a common interpretation of the 3Rs principles in animal research Open
View article: Editorial: New approach methods in immunology
Editorial: New approach methods in immunology Open
View article: Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells
Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells Open
View article: In silico-in vitro modeling to uncover cues involved in establishing microglia identity: TGF-β3 and laminin can drive microglia signature gene expression
In silico-in vitro modeling to uncover cues involved in establishing microglia identity: TGF-β3 and laminin can drive microglia signature gene expression Open
Microglia are the resident macrophages of the central nervous system (CNS) and play a key role in CNS development, homeostasis, and disease. Good in vitro models are indispensable to study their cellular biology, and although much progress…
View article: P2Y6 receptor-mediated signaling amplifies TLR-induced pro-inflammatory responses in microglia
P2Y6 receptor-mediated signaling amplifies TLR-induced pro-inflammatory responses in microglia Open
TLR-induced signaling initiates inflammatory responses in cells of the innate immune system. These responses are amongst others characterized by the secretion of high levels of pro-inflammatory cytokines, which are tightly regulated and ad…
View article: Two-Photon Polymerization of 2.5D and 3D Microstructures Fostering a Ramified Resting Phenotype in Primary Microglia
Two-Photon Polymerization of 2.5D and 3D Microstructures Fostering a Ramified Resting Phenotype in Primary Microglia Open
Microglia are the resident macrophages of the central nervous system and contribute to maintaining brain’s homeostasis. Current 2D “petri-dish” in vitro cell culturing platforms employed for microglia, are unrepresentative of the softness …
View article: Mycoplasma pneumoniae Compared to Streptococcus pneumoniae Avoids Induction of Proinflammatory Epithelial Cell Responses despite Robustly Inducing TLR2 Signaling
Mycoplasma pneumoniae Compared to Streptococcus pneumoniae Avoids Induction of Proinflammatory Epithelial Cell Responses despite Robustly Inducing TLR2 Signaling Open
Mycoplasma pneumoniae and Streptococcus pneumoniae are the most common bacterial causes of pneumonia in children. The clinical characteristics of pneumonia differ significantly between the two bacteria. We aimed to elucidate the difference…
View article: In vitro microglia models: the era of engineered cell microenvironments
In vitro microglia models: the era of engineered cell microenvironments Open
The most widely employed approach by cell biologists to performing in vitro cell culture assays is the one using 2D plastic culture ware systems, which allows reproducibility and ease of use. Moreover, this method is cost-effective. Howeve…
View article: Transcriptome analysis reveals the contribution of oligodendrocyte and radial glia‐derived cues for maintenance of microglia identity
Transcriptome analysis reveals the contribution of oligodendrocyte and radial glia‐derived cues for maintenance of microglia identity Open
Microglia are increasingly being recognized as druggable targets in neurodegenerative disorders, and good in vitro models are crucial to address cell biological questions. Major challenges are to recapitulate the complex microglial morphol…
View article: Exposure of Microglia to Interleukin-4 Represses NF-κB-Dependent Transcription of Toll-Like Receptor-Induced Cytokines
Exposure of Microglia to Interleukin-4 Represses NF-κB-Dependent Transcription of Toll-Like Receptor-Induced Cytokines Open
Interleukin (IL)-4 is a cytokine that affects both adaptive and innate immune responses. In the central nervous system, microglia express IL-4 receptors and it has been described that IL-4-exposed microglia acquire anti-inflammatory proper…
View article: Recombinant myelin oligodendrocyte glycoprotein quality modifies evolution of experimental autoimmune encephalitis in macaques
Recombinant myelin oligodendrocyte glycoprotein quality modifies evolution of experimental autoimmune encephalitis in macaques Open
View article: Lactoferrin Retargets Human Adenoviruses to TLR4 to Induce an Abortive NLRP3-Associated Pyroptotic Response in Human Phagocytes
Lactoferrin Retargets Human Adenoviruses to TLR4 to Induce an Abortive NLRP3-Associated Pyroptotic Response in Human Phagocytes Open
Despite decades of clinical and preclinical investigations, we still poorly grasp our innate immune response to human adenoviruses (HAdVs) and their vectors. In this study, we explored the impact of lactoferrin on three HAdV types that are…
View article: Transcriptional profiling of macaque microglia reveals an evolutionary preserved gene expression program
Transcriptional profiling of macaque microglia reveals an evolutionary preserved gene expression program Open
View article: Adverse immunostimulation caused by impurities: The dark side of biopharmaceuticals
Adverse immunostimulation caused by impurities: The dark side of biopharmaceuticals Open
Drug safety is an important issue, especially in the experimental phases of development. Adverse immunostimulation (AI) is sometimes encountered following treatment with biopharmaceuticals, which can be life‐threatening if it results in a …
View article: VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases
VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases Open
V‐type immunoglobulin domain‐containing suppressor of T‐cell activation (VISTA) is a negative checkpoint regulator (NCR) involved in inhibition of T cell‐mediated immunity. Expression changes of other NCRs (PD‐1, PD‐L1/L2, CTLA‐4) during i…
View article: An Overview of in vitro Methods to Study Microglia
An Overview of in vitro Methods to Study Microglia Open
Neuroinflammation is a common feature in neurodegenerative diseases and strategies to modulate neuroinflammatory processes are increasingly considered as therapeutic options. In such strategies, glia cells rather than neurons represent the…
View article: Fibronectin aggregates promote features of a classically and alternatively activated phenotype in macrophages
Fibronectin aggregates promote features of a classically and alternatively activated phenotype in macrophages Open
View article: Carbohydrate fatty acid monosulphate esters are safe and effective adjuvants for humoral responses
Carbohydrate fatty acid monosulphate esters are safe and effective adjuvants for humoral responses Open
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View article: Expression of IL-1β in rhesus EAE and MS lesions is mainly induced in the CNS itself
Expression of IL-1β in rhesus EAE and MS lesions is mainly induced in the CNS itself Open
View article: Inflammasome-Induced IL-1β Secretion in Microglia Is Characterized by Delayed Kinetics and Is Only Partially Dependent on Inflammatory Caspases
Inflammasome-Induced IL-1β Secretion in Microglia Is Characterized by Delayed Kinetics and Is Only Partially Dependent on Inflammatory Caspases Open
Inflammasomes are multiprotein complexes that link pathogen recognition and cellular stress to the processing of the proinflammatory cytokine interleukin-1β (IL-1β). Whereas inflammasome-mediated activation is heavily studied in hematopoie…