Jeffrey Shabanowitz
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View article: TTLL4 glutamyltransferase is a therapeutic target for NPM1-mutated Acute Myeloid Leukemia
TTLL4 glutamyltransferase is a therapeutic target for NPM1-mutated Acute Myeloid Leukemia Open
Mutations in nucleophosmin (NPM1) are identified in 35% of acute myeloid leukemia (AML) patients, but therapeutic strategies targeting this subtype of AML remain limited [PMID:23436734]. The most common mutation in NPM1, termed NPM1c, is a…
View article: O-Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis
O-Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis Open
The phytohormone auxin plays a pivotal role in promoting fruit initiation and growth upon fertilization in flowering plants. Upregulation of auxin signaling by genetic mutations or exogenous auxin treatment can induce seedless fruit format…
View article: Identification of Post-translationally Modified MHC Class I–Associated Peptides as Potential Cancer Immunotherapeutic Targets
Identification of Post-translationally Modified MHC Class I–Associated Peptides as Potential Cancer Immunotherapeutic Targets Open
Over the past 3 decades, the Hunt laboratory has developed advancements in mass spectrometry-based technologies to enable the identification of peptides bound to major histocompatibility complex (MHC) molecules. The MHC class I processing …
View article: TTLL4 glutamyltransferase is a therapeutic target for NPM1-mutated acute myeloid leukemia
TTLL4 glutamyltransferase is a therapeutic target for NPM1-mutated acute myeloid leukemia Open
NPM1-mutated acute myeloid leukemia (AML) is defined by aberrant cytoplasmic localization of the mutant NPM1c protein, and therapeutic strategies targeting this specific disease remain limited. Here, we identify TTLL4, a mono-glutamate glu…
View article: The protamines of the spider <i>Steatoda</i> sp. provide an example of liquid–liquid phase separation chromatin transitions during spermiogenesis
The protamines of the spider <i>Steatoda</i> sp. provide an example of liquid–liquid phase separation chromatin transitions during spermiogenesis Open
Although there is extensive information about sperm nuclear basic proteins (SNBP) in vertebrates, there is, by comparison, very little information in Arthropoda. This study aims to contribute to filling this gap by analyzing these proteins…
View article: Phosphorylation activates master growth regulator DELLA by promoting histone H2A binding at chromatin in Arabidopsis
Phosphorylation activates master growth regulator DELLA by promoting histone H2A binding at chromatin in Arabidopsis Open
DELLA proteins are conserved master growth regulators that play a central role in controlling plant development in response to internal and environmental cues. DELLAs function as transcription regulators, which are recruited to target prom…
View article: A Chemical Approach to Assess the Impact of Post-translational Modification on MHC Peptide Binding and Effector Cell Engagement
A Chemical Approach to Assess the Impact of Post-translational Modification on MHC Peptide Binding and Effector Cell Engagement Open
The human major histocompatibility complex (MHC) plays a pivotal role in the presentation of peptidic fragments from proteins, which can originate from self-proteins or from nonhuman antigens, such as those produced by viruses or bacteria.…
View article: The<i>O</i>-Fucosyltransferase SPINDLY Attenuates Auxin-Induced Fruit Growth by Inhibiting ARF6 and ARF8 binding to Coactivator Mediator Complex in<i>Arabidopsis</i>
The<i>O</i>-Fucosyltransferase SPINDLY Attenuates Auxin-Induced Fruit Growth by Inhibiting ARF6 and ARF8 binding to Coactivator Mediator Complex in<i>Arabidopsis</i> Open
The phytohormone auxin plays a pivotal role in promoting fruit initiation and growth upon fertilization in flowering plants. Upregulation of auxin signaling by genetic mutations or exogenous auxin treatment can induce seedless fruit format…
View article: The protamines of the noble false widow spider<i>Steatoda nobilis</i>provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis
The protamines of the noble false widow spider<i>Steatoda nobilis</i>provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis Open
While there is extensive information about sperm nuclear basic proteins (SNBP) in vertebrates, there is very little information about Arthropoda by comparison. This paper aims to contribute to filling this gap by analyzing these proteins i…
View article: Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency
Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency Open
Histone chaperones-structurally diverse, non-catalytic proteins enriched with acidic intrinsically disordered regions (IDRs)-protect histones from spurious nucleic acid interactions and guide their deposition into and out of nucleosomes. D…
View article: Protamines and the sperm nuclear basic proteins Pandora’s Box of insects
Protamines and the sperm nuclear basic proteins Pandora’s Box of insects Open
Insects are the largest group of animals when it comes to the number and diversity of species. Yet, with the exception of Drosophila, no information is currently available on the primary structure of their sperm nuclear basic proteins (SNB…
View article: Analysis of the interplay between MeCP2 and histone H1 during <i>in vitro</i> differentiation of human ReNCell neural progenitor cells
Analysis of the interplay between MeCP2 and histone H1 during <i>in vitro</i> differentiation of human ReNCell neural progenitor cells Open
An immortalized neural cell line derived from the human ventral mesencephalon, called ReNCell, and its MeCP2 knock out were used. With it, we characterized the chromatin compositional transitions undergone during differentiation, with spec…
View article: Analysis of the interplay between MeCP2 and histone H1 during <i>in vitro</i> differentiation of human ReNCell neural progenitor cells
Analysis of the interplay between MeCP2 and histone H1 during <i>in vitro</i> differentiation of human ReNCell neural progenitor cells Open
An immortalized neural cell line derived from the human ventral mesencephalon, called ReNCell, and its MeCP2 knock out were used. With it, we characterized the chromatin compositional transitions undergone during differentiation, with spec…
View article: Phosphorylation Promotes DELLA Activity by Enhancing Its Binding to Histone H2A at Target Chromatin in<i>Arabidopsis</i>
Phosphorylation Promotes DELLA Activity by Enhancing Its Binding to Histone H2A at Target Chromatin in<i>Arabidopsis</i> Open
DELLA proteins are conserved master growth regulators that play a central role in controlling plant development in response to internal and environmental cues. DELLAs function as transcription regulators, which are recruited to target prom…
View article: Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA charge mimicry to enhance chaperone efficiency
Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA charge mimicry to enhance chaperone efficiency Open
Histone chaperones–structurally diverse, non-catalytic proteins enriched with acidic intrinsically disordered regions (IDRs)–protect histones from spurious nucleic acid interactions and guide their deposition into and out of nucleosomes. D…
View article: Data from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia
Data from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia Open
Leukemias are highly immunogenic, but they have a low mutational load, providing few mutated peptide targets. Thus, the identification of alternative neoantigens is a pressing need. Here, we identify 36 MHC class I–associated peptide antig…
View article: Supplementary Tables 1 and 2 and Supplementary Figures 1 through 7 from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia
Supplementary Tables 1 and 2 and Supplementary Figures 1 through 7 from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia Open
Table S1. Summary of samples used for this study. Table S2. Antibodies used in the study. Figure S1. Mass spectra of all peptides referenced in figures 2 and 3. Figure S2. ETD Mass spectrum of RPPItQSSL containing an asymmetrically dimethy…
View article: Data from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia
Data from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia Open
Leukemias are highly immunogenic, but they have a low mutational load, providing few mutated peptide targets. Thus, the identification of alternative neoantigens is a pressing need. Here, we identify 36 MHC class I–associated peptide antig…
View article: Supplementary Tables 1 and 2 and Supplementary Figures 1 through 7 from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia
Supplementary Tables 1 and 2 and Supplementary Figures 1 through 7 from Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia Open
Table S1. Summary of samples used for this study. Table S2. Antibodies used in the study. Figure S1. Mass spectra of all peptides referenced in figures 2 and 3. Figure S2. ETD Mass spectrum of RPPItQSSL containing an asymmetrically dimethy…
View article: Evidence That Binding of Cyclic GMP to the Extracellular Domain of NKA (Sodium-Potassium ATPase) Mediates Natriuresis
Evidence That Binding of Cyclic GMP to the Extracellular Domain of NKA (Sodium-Potassium ATPase) Mediates Natriuresis Open
Background: Extracellular renal interstitial guanosine cyclic 3’,5’-monophosphate (cGMP) inhibits renal proximal tubule (RPT) sodium (Na + ) reabsorption via Src (Src family kinase) activation. Through which target extracellular cGMP acts …
View article: SPINDLY<i>O</i>-fucosylates nuclear and cytoplasmic proteins involved in diverse cellular processes in plants
SPINDLY<i>O</i>-fucosylates nuclear and cytoplasmic proteins involved in diverse cellular processes in plants Open
SPINDLY (SPY) is a novel nucleocytoplasmic protein O-fucosyltransferase that regulates target protein activity or stability via O-fucosylation of specific Ser/Thr residues. Previous genetic studies indicate that AtSPY regulates plant devel…
View article: Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation
Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation Open
Electron transfer dissociation (ETD) is an analytically useful tool for primary structure interrogation of intact proteins, but its utility is limited by higher-order reactions with the products. To inhibit these higher-order reactions, fi…
View article: Nitrogen-Containing Aromatic Radical Anions Perform Multiple Proton and Electron Transfers Near-Simultaneously with Multiply Protonated Cations
Nitrogen-Containing Aromatic Radical Anions Perform Multiple Proton and Electron Transfers Near-Simultaneously with Multiply Protonated Cations Open
Gas phase ion/ion reactions between singly charged radical reagent anions and multiply charged cation precursors primarily result in either proton or electron transfer. These ion/ion reactions have been extensively studied for bioanalysis,…
View article: Reinspection of a Clinical Proteomics Tumor Analysis Consortium (CPTAC) Dataset with Cloud Computing Reveals Abundant Post-Translational Modifications and Protein Sequence Variants
Reinspection of a Clinical Proteomics Tumor Analysis Consortium (CPTAC) Dataset with Cloud Computing Reveals Abundant Post-Translational Modifications and Protein Sequence Variants Open
The Clinical Proteomic Tumor Analysis Consortium (CPTAC) has provided some of the most in-depth analyses of the phenotypes of human tumors ever constructed. Today, the majority of proteomic data analysis is still performed using software h…
View article: Direct Target Site Identification of a Sulfonyl–Triazole Covalent Kinase Probe by LC-MS Chemical Proteomics
Direct Target Site Identification of a Sulfonyl–Triazole Covalent Kinase Probe by LC-MS Chemical Proteomics Open
Chemical proteomics is widely used for the global investigation of protein activity and binding of small molecule ligands. Covalent probe binding and inhibition are assessed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) t…
View article: Tumor Infiltrating Lymphocytes Target HLA-I Phosphopeptides Derived From Cancer Signaling in Colorectal Cancer
Tumor Infiltrating Lymphocytes Target HLA-I Phosphopeptides Derived From Cancer Signaling in Colorectal Cancer Open
There is a pressing need for novel immunotherapeutic targets in colorectal cancer (CRC). Cytotoxic T cell infiltration is well established as a key prognostic indicator in CRC, and it is known that these tumor infiltrating lymphocytes (TIL…