Jefree Johari
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View article: Rodent-borne zoonotic diseases in Southeast Asia: A narrative review
Rodent-borne zoonotic diseases in Southeast Asia: A narrative review Open
Rodent-borne zoonotic diseases, including hantavirus pulmonary syndrome, leptospirosis, and rickettsiosis, significantly impact public health. However, there is a limited understanding of these diseases in Southeast Asia, a region emerging…
View article: Association between Symptoms and IgM/IgG Levels Following Pre-Omicron and Omicron SARS-CoV-2 Breakthrough Infections
Association between Symptoms and IgM/IgG Levels Following Pre-Omicron and Omicron SARS-CoV-2 Breakthrough Infections Open
View article: Cohort Profile: Immune Responses to SARS-COV-2 Vaccination and Infection in a Longitudinal Sampling Amidst the COVID-19 Pandemic (LONGTONG-SARS2) in Malaysia
Cohort Profile: Immune Responses to SARS-COV-2 Vaccination and Infection in a Longitudinal Sampling Amidst the COVID-19 Pandemic (LONGTONG-SARS2) in Malaysia Open
Purpose This prospective, longitudinal study aims to evaluate the durability and functionality of SARS-CoV-2 Ancestral strain (Wuhan-Hu-1)-specific immune responses induced by COVID-19 vaccination and natural infection over a 12-month peri…
View article: MERS-CoV seroconversion amongst Malaysian Hajj pilgrims returning from the Middle East, 2016–2018: results from the MERCURIAL multiyear prospective cohort study
MERS-CoV seroconversion amongst Malaysian Hajj pilgrims returning from the Middle East, 2016–2018: results from the MERCURIAL multiyear prospective cohort study Open
Prospective cohort study to investigate the potential exposure to the Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) following Hajj pilgrims is still very limited. Here, we report the antibody seroconversion study results obtained…
View article: Decrease in RT-PCR Ct values among SARS-CoV-2 positive samples during the emergence of the B.1.617.2 (Delta) variant in Malaysia
Decrease in RT-PCR Ct values among SARS-CoV-2 positive samples during the emergence of the B.1.617.2 (Delta) variant in Malaysia Open
The RT-PCR cycle threshold (Ct) value for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is inversely proportionate to the virus load in the patient’s specimen. These values could be beneficial to b…
View article: Emergence of B.1.524(G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave
Emergence of B.1.524(G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave Open
The COVID-19 pandemic first emerged in Malaysia in Jan 2020. As of 12th Sept 2021, 1,979,698 COVID-19 cases that occurred over three major epidemic waves were confirmed. The virus contributing to the three epidemic waves has not been well-…
View article: Multiyear prospective cohort study to evaluate the risk potential of MERS-CoV infection among Malaysian Hajj pilgrims (MERCURIAL): a study protocol
Multiyear prospective cohort study to evaluate the risk potential of MERS-CoV infection among Malaysian Hajj pilgrims (MERCURIAL): a study protocol Open
Introduction Middle East respiratory syndrome (MERS) is a viral respiratory infection caused by the MERS-CoV. MERS was first reported in the Kingdom of Saudi Arabia in 2012. Every year, the Hajj pilgrimage to Mecca attracts more than two m…
View article: Multiplex Sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM)
Multiplex Sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM) Open
Various methods have been developed for rapid and high throughput full genome sequencing of SARS-CoV-2. Here, we described a protocol for targeted multiplex full genome sequencing of SARS-CoV-2 genomic RNA directly extracted from human nas…
View article: Investigation into an annonymous, unclaimed Japanese encephalitis virus strain and, the biosafety and biosecurity lessons learnt.
Investigation into an annonymous, unclaimed Japanese encephalitis virus strain and, the biosafety and biosecurity lessons learnt. Open
View article: Dengue fever among febrile patients in Taiz City, Yemen during the 2016 war: Clinical manifestations, risk factors, and patients knowledge, attitudes, and practices toward the disease
Dengue fever among febrile patients in Taiz City, Yemen during the 2016 war: Clinical manifestations, risk factors, and patients knowledge, attitudes, and practices toward the disease Open
View article: Seroprevalence of Borrelia burgdorferi among the indigenous people (Orang Asli) of Peninsular Malaysia
Seroprevalence of Borrelia burgdorferi among the indigenous people (Orang Asli) of Peninsular Malaysia Open
Introduction: Lyme disease has been well-described in the North America and European countries. However, information is still very limited in the developing countries including Malaysia. The Orang Asli (OA), the indigenous people of Penins…
View article: Dengue Outbreak during Ongoing Civil War, Taiz, Yemen
Dengue Outbreak during Ongoing Civil War, Taiz, Yemen Open
We identified dengue in ≈51% of patients given a clinical diagnosis of suspected dengue in Taiz, Yemen, during 2016. The cosmopolitan genotype of dengue virus type 2 was most common; viruses appeared to have originated in Saudi Arabia. Dam…
View article: Sera of patients with systemic lupus erythematosus cross‐neutralizes dengue viruses
Sera of patients with systemic lupus erythematosus cross‐neutralizes dengue viruses Open
Dengue is the most prevalent mosquito‐borne disease in Southeast Asia, where the incidence of systemic lupus erythematosus (SLE) is approximately 30 to 53 per 100,000. Severe dengue, however, is rarely reported among individuals with SLE. …
View article: Recovery of <i>Bordetella bronchiseptica</i> sequence type 82 and <i>B. pseudohinzii</i> from urban rats in Terengganu, Malaysia
Recovery of <i>Bordetella bronchiseptica</i> sequence type 82 and <i>B. pseudohinzii</i> from urban rats in Terengganu, Malaysia Open
Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future…
View article: Penicillin-Susceptible, Oxidase-Negative, Nonhemolytic, Nonmotile<i>Bacillus megaterium</i>in Disguise of<i>Bacillus anthracis</i>
Penicillin-Susceptible, Oxidase-Negative, Nonhemolytic, Nonmotile<i>Bacillus megaterium</i>in Disguise of<i>Bacillus anthracis</i> Open
Bacillus anthracis is a bacterial pathogen of major concern. The spores of this bacteria can survive harsh environmental conditions for extended periods and are well recognized as a potential bioterror weapon with significant implications.…
View article: Synovial Tissue Infection with<i>Burkholderia fungorum</i>
Synovial Tissue Infection with<i>Burkholderia fungorum</i> Open
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View article: The Use of NS1 Rapid Diagnostic Test and qRT-PCR to Complement IgM ELISA for Improved Dengue Diagnosis from Single Specimen
The Use of NS1 Rapid Diagnostic Test and qRT-PCR to Complement IgM ELISA for Improved Dengue Diagnosis from Single Specimen Open
View article: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification
Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification Open
Dengue is usually diagnosed by isolation of the virus, serology or molecular diagnostic methods. Several commercial kits for the diagnosis of dengue are existing, but concerns have arisen regarding to the affordability and performance char…
View article: Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification
Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification Open
A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min wit…