Jens Balke
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View article: Cy3‐Based Nanoviscosity Determination of Mucus: Effect of Mucus Collection Methods and Antibiotics Treatment
Cy3‐Based Nanoviscosity Determination of Mucus: Effect of Mucus Collection Methods and Antibiotics Treatment Open
The integrity of the protective mucus layer as a primary defense against pathogen invasion and microbial leakage into the intestinal epithelium can be compromised by the effects of antibiotics on the commensal microbiome. Changes in mucus …
View article: Osmolytes Modulate Photoactivation of Phytochrome: Probing Protein Hydration
Osmolytes Modulate Photoactivation of Phytochrome: Probing Protein Hydration Open
Phytochromes are bistable red/far-red light-responsive photoreceptor proteins found in plants, fungi, and bacteria. Light-activation of the prototypical phytochrome Cph1 from the cyanobacterium Synechocystis sp. PCC 6803 allows photoisomer…
View article: Diffusion Analysis of NAnoscopic Ensembles: A Tracking‐Free Diffusivity Analysis for NAnoscopic Ensembles in Biological Samples and Nanotechnology (Small 16/2023)
Diffusion Analysis of NAnoscopic Ensembles: A Tracking‐Free Diffusivity Analysis for NAnoscopic Ensembles in Biological Samples and Nanotechnology (Small 16/2023) Open
NAnoscopic Ensembles Diffusion Analysis of NAnoscopic Ensembles (DANAE) offers an assumption- and tracking-free approach to determine the diffusivities of single molecules/particles despite high densities, fast diffusion or photobleaching/…
View article: Diffusion Analysis of NAnoscopic Ensembles: A Tracking‐Free Diffusivity Analysis for NAnoscopic Ensembles in Biological Samples and Nanotechnology
Diffusion Analysis of NAnoscopic Ensembles: A Tracking‐Free Diffusivity Analysis for NAnoscopic Ensembles in Biological Samples and Nanotechnology Open
The rapid development of microscopic techniques over the past decades enables the establishment of single molecule fluorescence imaging as a powerful tool in biological and biomedical sciences. Single molecule fluorescence imaging allows t…
View article: Long-Distance Protonation-Conformation Coupling in Phytochrome Species
Long-Distance Protonation-Conformation Coupling in Phytochrome Species Open
Phytochromes are biological red/far-red light sensors found in many organisms. The connection between photoconversion and the cellular output signal involves light-mediated global structural changes in the interaction between the photosens…
View article: Author Correction: QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins
Author Correction: QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins Open
View article: QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins
QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins Open
View article: Improved fluorescent phytochromes for in situ imaging
Improved fluorescent phytochromes for in situ imaging Open
Modern biology investigations on phytochromes as near-infrared fluorescent pigments pave the way for the development of new biosensors, as well as for optogenetics and in vivo imaging tools. Recently, near-infrared fluorescent proteins (NI…
View article: Data from Publication
Data from Publication Open
Data from MDPI Publication: Long-Distance Protonation-Conformation Coupling in Phytochrome Species
View article: The redox-coupled proton-channel opening in cytochrome <i>c</i> oxidase
The redox-coupled proton-channel opening in cytochrome <i>c</i> oxidase Open
The interplay of cytochrome c oxidase's cofactor electrostatics, long-range conformational changes, H-bond rearrangement, and water dynamics enables transient proton-channel activation.
View article: Electronation‐dependent structural change at the proton exit side of cytochrome <i>c</i> oxidase as revealed by site‐directed fluorescence labeling
Electronation‐dependent structural change at the proton exit side of cytochrome <i>c</i> oxidase as revealed by site‐directed fluorescence labeling Open
Cytochrome c oxidase (C c O), the terminal enzyme of the respiratory chain of mitochondria and many aerobic prokaryotes that function as a redox‐coupled proton pump, catalyzes the reduction of molecular oxygen to water. As part of the resp…
View article: Oxidative Stress Imaging: Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range Using Fluorescence Lifetime Imaging (Small 23/2018)
Oxidative Stress Imaging: Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range Using Fluorescence Lifetime Imaging (Small 23/2018) Open
In article number 1800310, Nan Ma, Ulrike Alexiev, and co-workers develop a sensitive and reproducible fluorescence lifetime based imaging assay (FLIM-ROX) for reactive oxygen species (ROS) detection that allows the investigation of low ce…
View article: Pitfalls in using fluorescence tagging of nanomaterials: tecto‐dendrimers in skin tissue as investigated by Cluster‐FLIM
Pitfalls in using fluorescence tagging of nanomaterials: tecto‐dendrimers in skin tissue as investigated by Cluster‐FLIM Open
Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug‐delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier …
View article: Author Index
Author Index Open
View article: Light and pH-induced Changes in Structure and Accessibility of Transmembrane Helix B and Its Immediate Environment in Channelrhodopsin-2
Light and pH-induced Changes in Structure and Accessibility of Transmembrane Helix B and Its Immediate Environment in Channelrhodopsin-2 Open
View article: Application of Single Molecule Fluorescence Microscopy to Characterize the Penetration of a Large Amphiphilic Molecule in the Stratum Corneum of Human Skin
Application of Single Molecule Fluorescence Microscopy to Characterize the Penetration of a Large Amphiphilic Molecule in the Stratum Corneum of Human Skin Open
We report here on the application of laser-based single molecule total internal reflection fluorescence microscopy (TIRFM) to study the penetration of molecules through the skin. Penetration of topically applied drug molecules is often obs…
View article: A Universal Particle Image Correlation Spectroscopy (UPICS) for the Analysis of Fast and Densely Diffusing Particles
A Universal Particle Image Correlation Spectroscopy (UPICS) for the Analysis of Fast and Densely Diffusing Particles Open