Jeremy A. Hirota
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View article: A Stiffness-Gated YAP–β-Catenin Axis Orchestrates AXIN2 Expression in Metastatic Breast Cancer
A Stiffness-Gated YAP–β-Catenin Axis Orchestrates AXIN2 Expression in Metastatic Breast Cancer Open
View article: A mixing system for uniform, reproducible viscous bioink preparation
A mixing system for uniform, reproducible viscous bioink preparation Open
Rationale: Extrusion 3D bioprinting is an additive manufacturing tissue engineering technique that uses cell-laden viscous biomaterials known as bioinks. Manually mixing cell suspensions into viscous biomaterials can be challenging due to …
View article: Metatranscriptomic profiling reveals pathogen and host response signatures of pediatric acute sinusitis and upper respiratory infection
Metatranscriptomic profiling reveals pathogen and host response signatures of pediatric acute sinusitis and upper respiratory infection Open
Background Acute sinusitis (AS) is a frequent cause of antibiotic prescriptions in children. Distinguishing bacterial AS from common viral upper respiratory infections (URIs) is crucial to prevent unnecessary antibiotic use but is challeng…
View article: Top 10 priorities for chronic cough: Canadian James Lind Alliance Priority-Setting Partnership (CAN-COUGH)
Top 10 priorities for chronic cough: Canadian James Lind Alliance Priority-Setting Partnership (CAN-COUGH) Open
Background Chronic cough significantly impacts individuals’ quality of life and poses challenges for healthcare providers due to limited licensed treatments, side effects of available medications and difficulty accessing nonpharmacological…
View article: Open-source, three-dimensionally printed manifolds for exposure studies using human airway epithelial cells
Open-source, three-dimensionally printed manifolds for exposure studies using human airway epithelial cells Open
Rationale In vitro studies using air–liquid interface (ALI) cultures enable controlled investigation of human airway epithelial cell (HAEC) responses to clinically relevant exposures. Commercial in vitro exposure systems provide precise an…
View article: Diagnostic potential of genomic blood biomarkers of pulmonary fibrosis in a prospective cohort
Diagnostic potential of genomic blood biomarkers of pulmonary fibrosis in a prospective cohort Open
Fibrotic interstitial lung diseases (ILDs) result from excessive deposition of extracellular matrix (ECM) proteins in the lung, causing irreversible damage to the lung architecture. Clinical management of ILDs differs depending on the diag…
View article: Open-source 3D printed manifolds for exposure studies using human airway epithelial cells
Open-source 3D printed manifolds for exposure studies using human airway epithelial cells Open
Rationale Inhalation of airborne stimuli can damage the airway epithelium, increasing the risk of developing respiratory or systemic diseases. In vitro studies using air-liquid interface cell cultures enable controlled investigation of cel…
View article: Dried Cannabis Use, Tobacco Smoking, and COVID-19 Infection: Findings from a Longitudinal Observational Cohort Study
Dried Cannabis Use, Tobacco Smoking, and COVID-19 Infection: Findings from a Longitudinal Observational Cohort Study Open
Objective: The potential impact of cigarette and cannabis smoking on COVID-19 infection outcomes is not well understood. We investigated the association between combustible tobacco use and dried cannabis use with COVID-19 infection in a lo…
View article: COVID‐19 severity gradient differentially dysregulates clinically relevant drug processing genes in nasopharyngeal swab samples
COVID‐19 severity gradient differentially dysregulates clinically relevant drug processing genes in nasopharyngeal swab samples Open
Aim Understanding how COVID‐19 impacts the expression of clinically relevant drug metabolizing enzymes and membrane transporters (DMETs) is vital for addressing potential safety and efficacy concerns related to systemic and peripheral drug…
View article: Deciphering the Mechanotransduction Symphony: Stiffness-Dependent Interplay of YAP and β-Catenin in Breast Cancer Metastasis
Deciphering the Mechanotransduction Symphony: Stiffness-Dependent Interplay of YAP and β-Catenin in Breast Cancer Metastasis Open
Metastatic breast cancer poses a formidable clinical challenge, demanding a comprehensive understanding of the intricate signaling networks orchestrating disease progression. Herein, our findings revealed a pronounced increase in the nucle…
View article: Nasopharyngeal metatranscriptomics reveals host-pathogen signatures of pediatric sinusitis
Nasopharyngeal metatranscriptomics reveals host-pathogen signatures of pediatric sinusitis Open
Acute sinusitis (AS) is the fifth leading cause of antibiotic prescriptions in children. Distinguishing bacterial AS from common viral upper respiratory infections in children is crucial to prevent unnecessary antibiotic use but is challen…
View article: SARS-CoV-2 3CLpro Main Protease Drives Cytoskeletal Reorganization and Tunnelling Nanotube Formation for Stealth Intercellular Infection
SARS-CoV-2 3CLpro Main Protease Drives Cytoskeletal Reorganization and Tunnelling Nanotube Formation for Stealth Intercellular Infection Open
To identify and characterize roles for SARS-CoV-2 3CL pro main protease in promoting viral infection and dissemination, we employed Inactive Catalytic Domain Capture and proteomics to identify host cell interactors and substrates in the in…
View article: A200 EXPOSURE TO CANNABIS SMOKE <i>IN UTERO</i> AFFECTS THE DEVELOPMENT OF THE ENDOCANNABINOID SYSTEM IN THE GASTROINTESTINAL TRACT
A200 EXPOSURE TO CANNABIS SMOKE <i>IN UTERO</i> AFFECTS THE DEVELOPMENT OF THE ENDOCANNABINOID SYSTEM IN THE GASTROINTESTINAL TRACT Open
Background Recreational use of cannabis has been legalized in Canada since 2018. Increasing numbers of women are using cannabis while pregnant, with reported rates ampersand:003E10%. There is a disconnect with societal perceptions, with up…
View article: GelMA-Carbopol bioinks with low total solids content for high-fidelity extrusion 3D bioprinting of dynamic tissue biomimetics
GelMA-Carbopol bioinks with low total solids content for high-fidelity extrusion 3D bioprinting of dynamic tissue biomimetics Open
Extrusion 3D bioprinting is a technology that allows the deposition of cells within hydrogels in well-defined spatial patterns, facilitating the fabrication of tissue biomimetics. Gelatin methacrylate (GelMA)-based hydrogels are biocompati…
View article: Characterization of CXCL10 as a biomarker of respiratory tract infections detectable by open-source lateral flow immunoassay
Characterization of CXCL10 as a biomarker of respiratory tract infections detectable by open-source lateral flow immunoassay Open
Understanding core mechanisms common to respiratory tract viral pathogenesis and host-responses to infections may provide biomarkers for at-risk patient populations that guide interventions aimed at reducing morbidity, mortality, and econo…
View article: Deciphering the Mechanotransduction Symphony: Stiffness-Dependent Interplay of YAP and β-Catenin in Breast Cancer Metastasis
Deciphering the Mechanotransduction Symphony: Stiffness-Dependent Interplay of YAP and β-Catenin in Breast Cancer Metastasis Open
View article: High-Fidelity Extrusion Bioprinting of Low-Printability Polymers Using Carbopol as a Rheology Modifier
High-Fidelity Extrusion Bioprinting of Low-Printability Polymers Using Carbopol as a Rheology Modifier Open
Extrusion three-dimensional (3D) bioprinting is a promising technology with many applications in the biomedical and tissue engineering fields. One of the key limitations for the widespread use of this technology is the narrow window of pri…
View article: Challenges and Opportunities for Commercializing Technologies in the Pulmonary Arena: An Official American Thoracic Society Report
Challenges and Opportunities for Commercializing Technologies in the Pulmonary Arena: An Official American Thoracic Society Report Open
"Translational medicine" has been a buzzword for over two decades. The concept was intended to be lofty, to reflect a new "bench-to-bedside" approach to basic and clinical research that would bridge fields, close gaps, accelerate innovatio…
View article: Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenzae isolates associated with paediatric otitis media and upper respiratory infection
Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenzae isolates associated with paediatric otitis media and upper respiratory infection Open
View article: TNF-α and Poly(I:C) induction of A20 and activation of NF-κB signaling are independent of ABCF1 in human airway epithelial cells
TNF-α and Poly(I:C) induction of A20 and activation of NF-κB signaling are independent of ABCF1 in human airway epithelial cells Open
ABCF1 is the most characterized member of the ABCF family in eukaryotes with proposed functions related to innate immunity in fibroblasts, macrophages, and epithelial cells. Currently, a mechanistic link between ABCF1 and immune responses …
View article: Irreversible PDMS bonding using flame activation of adhesives for fabrication of microfluidic and organ-on-chip devices
Irreversible PDMS bonding using flame activation of adhesives for fabrication of microfluidic and organ-on-chip devices Open
Polydimethylsiloxane (PDMS) is widely used for microfluidics fabrication in many disciplines due to its ease of use in soft lithography and its ability to bond liquid-tight seals. A variety of PDMS-PDMS bonding methods exist, but each may …
View article: Cannabis smoke suppresses antiviral immune responses to influenza A in mice
Cannabis smoke suppresses antiviral immune responses to influenza A in mice Open
Rationale Despite its increasingly widespread use, little is known about the impact of cannabis smoking on the response to viral infections like influenza A virus (IAV). Many assume that cannabis smoking will disrupt antiviral responses in…
View article: Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures
Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures Open
Soluble signaling molecules and extracellular matrix (ECM) regulate cell dynamics in various biological processes. Wound healing assays are widely used to study cell dynamics in response to physiological stimuli. However, traditional scrat…
View article: A roadmap for developing and engineering <i>in vitro</i> pulmonary fibrosis models
A roadmap for developing and engineering <i>in vitro</i> pulmonary fibrosis models Open
Idiopathic pulmonary fibrosis (IPF) is a severe form of pulmonary fibrosis. IPF is a fatal disease with no cure and is challenging to diagnose. Unfortunately, due to the elusive etiology of IPF and a late diagnosis, there are no cures for …
View article: Myeloid‐specific deletion of activating transcription factor 6 alpha increases <scp>CD11b</scp><sup>+</sup> macrophage subpopulations and aggravates lung fibrosis
Myeloid‐specific deletion of activating transcription factor 6 alpha increases <span>CD11b</span><sup>+</sup> macrophage subpopulations and aggravates lung fibrosis Open
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial lung disease of unknown etiology. The accumulation of macrophages is associated with disease pathogenesis. The unfolded protein response (UPR) has been li…
View article: TNF-α and Poly(I:C) induction of A20 and activation of NF-κB signaling are independent of ABCF1 in human airway epithelial cells
TNF-α and Poly(I:C) induction of A20 and activation of NF-κB signaling are independent of ABCF1 in human airway epithelial cells Open
ABCF1 is the most characterized member of the ABCF family in eukaryotes with proposed functions related to innate immunity in fibroblasts, macrophages, and epithelial cells. Currently, a mechanistic link between ABCF1 and immune responses …
View article: Comparative transcriptomic analysis of nasopharyngeal swabs from individuals with and without COVID-19
Comparative transcriptomic analysis of nasopharyngeal swabs from individuals with and without COVID-19 Open
This dataset contains the results of a transcriptomic (RNA-seq) analysis of nasopharyngeal swabs collected from 50 individuals with COVID-19 and 13 individuals without COVID-19 based on clinical qPCR testing. COVID-19 positive individuals …
View article: Additional file 1 of Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenza isolates associated with paediatric otitis media and upper respiratory infection
Additional file 1 of Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenza isolates associated with paediatric otitis media and upper respiratory infection Open
Additional file 1: Table S1. Clinical metadata. Table S2. Kraken 2 contig classifications weighted by contig length (% total assembly size) for S. pneumoniae isolates. Table S3. Assembly statistics from Pathogenwatch for S. pneumoniae isol…
View article: Additional file 1 of Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenzae isolates associated with paediatric otitis media and upper respiratory infection
Additional file 1 of Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenzae isolates associated with paediatric otitis media and upper respiratory infection Open
Additional file 1: Table S1. Clinical metadata. Table S2. Kraken 2 contig classifications weighted by contig length (% total assembly size) for S. pneumoniae isolates. Table S3. Assembly statistics from Pathogenwatch for S. pneumoniae isol…
View article: The CaT stretcher: An open-source system for delivering uniaxial strain to cells and tissues (CaT)
The CaT stretcher: An open-source system for delivering uniaxial strain to cells and tissues (CaT) Open
Integration of mechanical cues in conventional 2D or 3D cell culture platforms is an important consideration for in vivo and ex vivo models of lung health and disease. Available commercial and published custom-made devices are frequently l…