Jeremy G. Owen
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View article: DiscERN: An Automated Genome Mining Tool for the Discovery of Evolutionarily Related Natural Products
DiscERN: An Automated Genome Mining Tool for the Discovery of Evolutionarily Related Natural Products Open
Targeted genome mining to expand known families of natural products is a powerful strategy for discovering bioactive compounds, yet it remains a significant bioinformatics challenge. While tools exist for de novo biosynthetic gene cluster …
View article: Microbial communities associated with marine sponges from diverse geographic locations harbor biosynthetic novelty
Microbial communities associated with marine sponges from diverse geographic locations harbor biosynthetic novelty Open
Marine sponges are a prolific source of biologically active small molecules, many of which originate from sponge-associated bacteria. Identifying the producing bacteria is a key step in developing sustainable routes for the production of t…
View article: Calcium‐Dependent Lipopeptide Antibiotics against Drug‐Resistant Pathogens Discovered via Host‐Dependent Heterologous Expression of a Cloned Biosynthetic Gene Cluster
Calcium‐Dependent Lipopeptide Antibiotics against Drug‐Resistant Pathogens Discovered via Host‐Dependent Heterologous Expression of a Cloned Biosynthetic Gene Cluster Open
Historically, small molecules biosynthesised by bacteria have been an excellent source for antibacterial drugs. Today, however, the rediscovery of known compounds is a significant hurdle to developing new antimicrobials. Here we use a geno…
View article: Calcium‐Dependent Lipopeptide Antibiotics against Drug‐Resistant Pathogens Discovered via Host‐Dependent Heterologous Expression of a Cloned Biosynthetic Gene Cluster
Calcium‐Dependent Lipopeptide Antibiotics against Drug‐Resistant Pathogens Discovered via Host‐Dependent Heterologous Expression of a Cloned Biosynthetic Gene Cluster Open
Historically, small molecules biosynthesised by bacteria have been an excellent source for antibacterial drugs. Today, however, the rediscovery of known compounds is a significant hurdle to developing new antimicrobials. Here we use a geno…
View article: Microbial communities associated with marine sponges from diverse geographic locations harbour biosynthetic novelty
Microbial communities associated with marine sponges from diverse geographic locations harbour biosynthetic novelty Open
Marine sponges are a prolific source of biologically active small molecules, many of which originate from sponge-associated microbes. Identifying the producing microbes is a key challenge in developing sustainable routes for production and…
View article: Metagenomic domain substitution for the high-throughput modification of non-ribosomal peptide analogues
Metagenomic domain substitution for the high-throughput modification of non-ribosomal peptide analogues Open
Non-ribosomal peptides are a diverse and medically important group of natural products. They are biosynthesised by modular non-ribosomal peptide synthetase (NRPS) assembly-lines in which domains from each module act in concert to incorpora…
View article: Metagenomic domain substitution for the high-throughput modification of non-ribosomal peptide analogues
Metagenomic domain substitution for the high-throughput modification of non-ribosomal peptide analogues Open
Non-ribosomal peptides are a diverse and medically important group of natural products. They are biosynthesised by modular non-ribosomal peptide synthetase (NRPS) assembly-lines in which domains from each module act in concert to incorpora…
View article: A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening
A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening Open
Summary Functional screening of environmental DNA (eDNA) libraries is a potentially powerful approach to discover enzymatic “unknown unknowns”, but is usually heavily biased toward the tiny subset of genes preferentially transcribed and tr…
View article: Streptomyces Biosynthetic Gene Clusters
Streptomyces Biosynthetic Gene Clusters Open
A collection of genbank files 11975 biosynthetic gene clusters from generated using antiSMASH v6.0.1
View article: Streptomyces Biosynthetic Gene Clusters
Streptomyces Biosynthetic Gene Clusters Open
A collection of genbank files 11975 biosynthetic gene clusters from generated using antiSMASH v6.0.1
View article: A Genomic Survey of the Natural Product Biosynthetic Potential of Actinomycetes Isolated from New Zealand Lichens
A Genomic Survey of the Natural Product Biosynthetic Potential of Actinomycetes Isolated from New Zealand Lichens Open
Lichens are home to complex and distinctive microbial cohorts that have not been extensively explored for the ability to produce novel secondary metabolites. Here, we isolate and obtain genome sequence data for 322 actinomycetes from New Z…
View article: A genomic survey of the natural product biosynthetic potential of actinomycetes isolated from New Zealand lichens.
A genomic survey of the natural product biosynthetic potential of actinomycetes isolated from New Zealand lichens. Open
assembled contigs, antismash json files
View article: Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective
Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective Open
There is a real consensus that new antibiotics are urgently needed and are the best chance for combating antibiotic resistance. The phylum Actinobacteria is one of the main producers of new antibiotics, with a recent paradigm shift whereby…
View article: Draft Genome Sequence of a Novel <i>Rhizobium</i> Species Isolated from the Marine Macroalga <i>Codium fragile</i> (Oyster Thief)
Draft Genome Sequence of a Novel <i>Rhizobium</i> Species Isolated from the Marine Macroalga <i>Codium fragile</i> (Oyster Thief) Open
In the process of studying the relationship between marine macroalgae and their bacterial symbionts, we isolated a new species of Rhizobium , which we designated Rhizobium sp. nov. C1 (for “ Codium 1”). Here, we report the complete genome …
View article: Complete Genome Sequence of a Novel Pseudomonas fluorescens Strain Isolated from the Flower of Kūmarahou (Pomaderris kumeraho)
Complete Genome Sequence of a Novel Pseudomonas fluorescens Strain Isolated from the Flower of Kūmarahou (Pomaderris kumeraho) Open
Kūmarahou ( Pomaderris kumeraho ) is a shrub endemic to New Zealand used in rongoā (traditional medicine). While studying the antimicrobial properties of kūmarahou, we isolated a new strain of Pseudomonas fluorescens , which we designated …
View article: Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT
Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT Open
A recently-validated and underexplored drug target in Mycobacterium tuberculosis is PptT, an essential phosphopantetheinyl transferase (PPTase) that plays a critical role in activating enzymes for both primary and secondary metabolism. Ppt…
View article: Inhibition of Indigoidine Synthesis as a High-throughput Colourimetric Screen for Antibiotics Targeting the Essential <em>Mycobacterium tuberculosis</em> Phosphopantetheinyl Transferase PptT
Inhibition of Indigoidine Synthesis as a High-throughput Colourimetric Screen for Antibiotics Targeting the Essential <em>Mycobacterium tuberculosis</em> Phosphopantetheinyl Transferase PptT Open
A recently-validated and underexplored drug target in Mycobacterium tuberculosis is PptT, an essential phosphopantetheinyl transferase (PPTase) that plays a critical role in activating enzymes for both primary and secondary metabolism. Ppt…
View article: Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway
Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway Open
Heterologous expression of an aromatic polyketide biosynthetic gene cluster recovered from a New Zealand soil metagenome library resulted in the discovery of new bioactive aureolic acids.
View article: Targeted Isolation of Rubrolides from the New Zealand Marine Tunicate Synoicum kuranui
Targeted Isolation of Rubrolides from the New Zealand Marine Tunicate Synoicum kuranui Open
Global natural products social (GNPS) molecular networking is a useful tool to categorize chemical space within samples and streamline the discovery of new natural products. Here, we demonstrate its use in chemically profiling the extract …
View article: Metagenomic exploration of the marine sponge mycale hentscheli uncovers multiple polyketide-producing bacterial symbionts
Metagenomic exploration of the marine sponge mycale hentscheli uncovers multiple polyketide-producing bacterial symbionts Open
© 2020 Storey et al. Marine sponges have been a prolific source of unique bioactive compounds that are presumed to act as a deterrent to predation. Many of these compounds have potential therapeutic applications; however, the lack of effic…
View article: Metagenomic exploration of the marine sponge mycale hentscheli uncovers multiple polyketide-producing bacterial symbionts
Metagenomic exploration of the marine sponge mycale hentscheli uncovers multiple polyketide-producing bacterial symbionts Open
© 2020 Storey et al. Marine sponges have been a prolific source of unique bioactive compounds that are presumed to act as a deterrent to predation. Many of these compounds have potential therapeutic applications; however, the lack of effic…
View article: Metagenomic Exploration of the Marine Sponge <i>Mycale hentscheli</i> Uncovers Multiple Polyketide-Producing Bacterial Symbionts
Metagenomic Exploration of the Marine Sponge <i>Mycale hentscheli</i> Uncovers Multiple Polyketide-Producing Bacterial Symbionts Open
Mycale hentscheli is a marine sponge that is rich in bioactive small molecules. Here, we use direct metagenomic sequencing to elucidate highly complete and contiguous genomes for the major symbiotic bacteria of this sponge. We identify com…
View article: Efficient rational modification of non-ribosomal peptides by adenylation domain substitution
Efficient rational modification of non-ribosomal peptides by adenylation domain substitution Open
Non-ribosomal peptide synthetase (NRPS) enzymes form modular assembly-lines, wherein each module typically governs the incorporation of a specific amino acid or related monomer into a short peptide product. Modules are comprised of one or …
View article: Proteomics quantifies protein expression changes in a model cnidarian colonised by a thermally tolerant but suboptimal symbiont
Proteomics quantifies protein expression changes in a model cnidarian colonised by a thermally tolerant but suboptimal symbiont Open
The acquisition of thermally tolerant algal symbionts by corals has been proposed as a natural or assisted mechanism of increasing coral reef resilience to anthropogenic climate change, but the cell-level processes determining the performa…
View article: Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens
Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens Open
Despite the wide availability of antibiotics, infectious diseases remain a leading cause of death worldwide 1 . In the absence of new therapies, mortality rates due to untreatable infections are predicted to rise more than tenfold by 2050.…