Jerker Widengren
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View article: All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins
All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins Open
Photobleaching is a general hurdle of fluorescence-based techniques especially in high-resolution microscopy that relies on prolonged and complex illumination. Strategies to reduce photobleaching require chemical modifications of the cell …
View article: A neoantigen-microbead platform for personalized T cell cancer vaccination
A neoantigen-microbead platform for personalized T cell cancer vaccination Open
Colorectal cancer (CRC) is a leading cause of cancer mortality and is characterized by a high tumor mutational burden, making it responsive to immunotherapy. We developed a bioinformatic and manufacturing pipeline for personalized cancer v…
View article: Multiplexed Near-IR Detection of Single-Molecule Fluorescence Fluctuations Using a Single Superconducting Nanowire Single-Photon Detector
Multiplexed Near-IR Detection of Single-Molecule Fluorescence Fluctuations Using a Single Superconducting Nanowire Single-Photon Detector Open
View article: Structured illumination flowmetry
Structured illumination flowmetry Open
In this study, we establish the concept of structured illumination flowmetry (SIF) through comprehensive numerical simulations. SIF can capture two-dimensional (2D) flow velocity fields from a single snapshot image of the emission pattern …
View article: Monitoring nucleoside metabolism in living cells with a nucleobase analogue <i>via</i> fluorescence lifetime imaging
Monitoring nucleoside metabolism in living cells with a nucleobase analogue <i>via</i> fluorescence lifetime imaging Open
FLIM of the nucleobase analogue 2CNqA inside living cells. Mono-exponential lifetime of the free nucleoside triphosphate analogue changes upon cellular uptake to a bi-exponential decay that indicates incorporation into RNA.
View article: Photophysical structured illumination velocimetry based on the long-lasting emission response of lanthanide luminescent nanoparticles
Photophysical structured illumination velocimetry based on the long-lasting emission response of lanthanide luminescent nanoparticles Open
Photophysical structured illumination velocimetry (PP-SIV): a snapshot-based imaging approach for mapping 2D flow velocity fields using luminescent probes and structured excitation, validated via numerical simulations.
View article: A multiband NIR upconversion core-shell design for enhanced light harvesting of silicon solar cells
A multiband NIR upconversion core-shell design for enhanced light harvesting of silicon solar cells Open
Exploring lanthanide light upconversion (UC) has emerged as a promising strategy to enhance the near-infrared (NIR) responsive region of silicon solar cells (SSCs). However, its practical application under normal sunlight conditions has be…
View article: Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking
Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking Open
MINimal photon FLUXes (MINFLUX) offers super-resolution microscopy (SRM) with nanometer localization precision, with more relaxed fluorophore brightness and photostability requirements than for other SRM techniques. Nonetheless, low locali…
View article: Photoisomerization of heptamethine cyanine (Cy7) dyes: A theoretical study
Photoisomerization of heptamethine cyanine (Cy7) dyes: A theoretical study Open
In this study, density functional theory (DFT) combined with time-dependent (TD) DFT calculations were employed to investigate the photoisomerization reaction kinetics of two near infrared (NIR) heptamethine cyanine (Cy7-NH3 and Cy7-SO3) d…
View article: Photoredox-Catalyzed Radical Coupling of C7-Chloromethyl-Substituted Thiazolino Ring-Fused 2-Pyridones with Quinoxalinones
Photoredox-Catalyzed Radical Coupling of C7-Chloromethyl-Substituted Thiazolino Ring-Fused 2-Pyridones with Quinoxalinones Open
We have developed an Ir(PPy)3 photoredox-catalyzed cross-coupling reaction that allows installation of quinoxalinones at the C7 position of thiazolino ring-fused 2-pyridones (TRPs) under mild conditions. The methodology tolerate…
View article: Interplay between a Heptamethine Cyanine Dye Sensitizer (IR806) and Lanthanide Upconversion Nanoparticles
Interplay between a Heptamethine Cyanine Dye Sensitizer (IR806) and Lanthanide Upconversion Nanoparticles Open
Lanthanide‐doped upconversion nanoparticles (UCNPs) have attractive emission properties but suffer from weak light‐absorbing capacities and thereby relatively low brightnesses. This motivates using strongly absorbing dye molecules as anten…
View article: Interplay between a heptamethine cyanine dye sensitizer (IR806) and lanthanide upconversion nanoparticles
Interplay between a heptamethine cyanine dye sensitizer (IR806) and lanthanide upconversion nanoparticles Open
This folder contains all raw data underlying the results presented in a manuscript, submitted to Angewandte Chemie, and entitled: Interplay between a heptamethine cyanine dye sensitizer (IR806) and lanthanide upconversion nanoparticles Aut…
View article: Antibody-Loading of Biological Nanocarrier Vesicles Derived from Red-Blood-Cell Membranes
Antibody-Loading of Biological Nanocarrier Vesicles Derived from Red-Blood-Cell Membranes Open
Antibodies, disruptive potent therapeutic agents against pharmacological targets, face a barrier in crossing immune systems and cellular membranes. To overcome these, various strategies have been explored including shuttling via liposomes …
View article: Non-fluorescent transient states of tyrosine as a basis for label-free protein conformation and interaction studies
Non-fluorescent transient states of tyrosine as a basis for label-free protein conformation and interaction studies Open
The amino acids tryptophan, tyrosine, and phenylalanine have been extensively used for different label-free protein studies, based on the intensity, lifetime, wavelength and/or polarization of their emitted fluorescence. Similar to most fl…
View article: Interplay between a heptamethine cyanine dye sensitizer (IR806) and lanthanide upconversion nanoparticles
Interplay between a heptamethine cyanine dye sensitizer (IR806) and lanthanide upconversion nanoparticles Open
This folder contains all raw data underlying the results presented in a manuscript, submitted to Angewandte Chemie, and entitled: Interplay between a heptamethine cyanine dye sensitizer (IR806) and lanthanide upconversion nanoparticles Aut…
View article: Morphology controlled synthesis of Fe<sup>3+</sup>-doped upconversion nanomaterials
Morphology controlled synthesis of Fe<sup>3+</sup>-doped upconversion nanomaterials Open
This work details the synthesis of paramagnetic upconversion nanoparticles doped with Fe 3+ in various morphologies via a thermal decomposition method, followed by comprehensive characterization using diverse analytical techniques.
View article: Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters
Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters Open
Reversible dark state transitions in fluorophores represent a limiting factor in fluorescence-based ultrasensitive spectroscopy, are a necessary basis for fluorescence-based super-resolution imaging, but may also offer additional, largely …
View article: Dataset to manuscript entitled "Non-fluorescent transient states of tyrosine - a basis for label-free protein conformation and interaction studies" submitted to Scientifc Reports
Dataset to manuscript entitled "Non-fluorescent transient states of tyrosine - a basis for label-free protein conformation and interaction studies" submitted to Scientifc Reports Open
This folder contains all raw data underlying the results presented in a manuscript, submitted to Scientifc Reports, and entitled: Non-fluorescent transient states of tyrosine - a basis for label-free protein conformation and interaction st…
View article: Dataset to manuscript entitled "Non-fluorescent transient states of tyrosine - a basis for label-free protein conformation and interaction studies" submitted to Scientifc Reports
Dataset to manuscript entitled "Non-fluorescent transient states of tyrosine - a basis for label-free protein conformation and interaction studies" submitted to Scientifc Reports Open
This folder contains all raw data underlying the results presented in a manuscript, submitted to Scientifc Reports, and entitled: Non-fluorescent transient states of tyrosine - a basis for label-free protein conformation and interaction st…
View article: Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy
Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy Open
Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were pr…
View article: Antibody-loading of biological nanocarrier vesicles derived from red-blood-cell membranes
Antibody-loading of biological nanocarrier vesicles derived from red-blood-cell membranes Open
Antibodies, disruptive potent therapeutic agents against pharmacological targets, face a barrier crossing immune-system and cellular-membranes. To overcome these, various strategies have been explored including shuttling via liposomes or b…
View article: Local monitoring of photosensitizer transient states provides feedback for enhanced efficiency and targeting selectivity in photodynamic therapy
Local monitoring of photosensitizer transient states provides feedback for enhanced efficiency and targeting selectivity in photodynamic therapy Open
View article: Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy
Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy Open
Photo-induced dark transient states of fluorophores can pose a problem in fluorescence spectroscopy. However, their typically long lifetimes also make them highly environment sensitive, suggesting fluorophores with prominent dark-state for…
View article: Suppression of Cation Intermixing Highly Boosts the Performance of Core–Shell Lanthanide Upconversion Nanoparticles
Suppression of Cation Intermixing Highly Boosts the Performance of Core–Shell Lanthanide Upconversion Nanoparticles Open
Lanthanide upconversion nanoparticles (UCNPs) have been extensively explored as biomarkers, energy transducers, and information carriers in wide-ranging applications in areas from healthcare and energy to information technology. In promoti…
View article: Local Monitoring of Photosensitizer Transient States Provides Feedback for Enhanced Efficiency and Targeting Selectivity in Photodynamic Therapy
Local Monitoring of Photosensitizer Transient States Provides Feedback for Enhanced Efficiency and Targeting Selectivity in Photodynamic Therapy Open
Photodynamic therapy (PDT) fundamentally relies on local generation of PDT precursor states in added photosensitizers (PS), particularly triplet and photo-radical states. Monitoring these states in situ can provide important feedback but i…
View article: Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy
Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy Open
This folder contains all raw data underlying the results presented in a manuscript, submitted for publication to Methods and Applications in Fluorescence, and entitled: Photo-physical characterization of h…
View article: Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy
Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy Open
This folder contains all raw data underlying the results presented in a manuscript, submitted for publication to Methods and Applications in Fluorescence, and entitled: Photo-physical characterization of h…
View article: Local Monitoring of Photosensitizer Transient States Provides Feedback for Enhanced Efficiency and Targeting Selectivity in Photodynamic Therapy.
Local Monitoring of Photosensitizer Transient States Provides Feedback for Enhanced Efficiency and Targeting Selectivity in Photodynamic Therapy. Open
This folder contains all raw data underlying the results presented in a manuscript, accepted for publication in Scientifc Reports, entitled: Local Monitoring of Photosensitizer Transient States Provides Feedback fo…
View article: Local Monitoring of Photosensitizer Transient States Provides Feedback for Enhanced Efficiency and Targeting Selectivity in Photodynamic Therapy.
Local Monitoring of Photosensitizer Transient States Provides Feedback for Enhanced Efficiency and Targeting Selectivity in Photodynamic Therapy. Open
This folder contains all raw data underlying the results presented in a manuscript, accepted for publication in Scientifc Reports, entitled: Local Monitoring of Photosensitizer Transient States Provides Feedback fo…
View article: Frequency-Domain Method for Characterization of Upconversion Luminescence Kinetics
Frequency-Domain Method for Characterization of Upconversion Luminescence Kinetics Open
The frequency-domain (FD) method provides an alternative to the commonly used time-domain (TD) approach in characterizing the luminescence kinetics of luminophores, with its own strengths, e.g., the capability to decouple multiple lifetime…