Jerry Ståhlberg
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View article: 1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium
1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium Open
View article: The first crystal structure of a family 45 glycoside hydrolase from a brown‐rot fungus, <i>Gloeophyllum trabeum</i> <scp>GtCel45A</scp>
The first crystal structure of a family 45 glycoside hydrolase from a brown‐rot fungus, <i>Gloeophyllum trabeum</i> <span>GtCel45A</span> Open
Here we describe the first crystal structure of a beta‐1,4‐endoglucanase from a brown‐rot fungus, Gloeophyllum trabeum Gt Cel45A, which belongs to subfamily C of glycoside hydrolase family 45 (GH45). Gt Cel45A is ~ 18 kDa in size and the c…
View article: Thermoprotection by a cell membrane–localized metacaspase in a green alga
Thermoprotection by a cell membrane–localized metacaspase in a green alga Open
Caspases are restricted to animals, while other organisms, including plants, possess metacaspases (MCAs), a more ancient and broader class of structurally related yet biochemically distinct proteases. Our current understanding of plant MCA…
View article: Chiral recognition mechanism of cellobiohydrolase Cel7A for ligands based on the β‐blocker propranolol: The effect of explicit water molecules on binding and selectivities
Chiral recognition mechanism of cellobiohydrolase Cel7A for ligands based on the β‐blocker propranolol: The effect of explicit water molecules on binding and selectivities Open
Proteins are useful chiral selectors. In order to understand the recognition mechanism and chiral discrimination, the binding of the ( R )‐ and ( S )‐enantiomers of a series of designed amino alcohol inhibitors based on propranolol to cell…
View article: Structure–function study of a Ca <sup>2+</sup> -independent metacaspase involved in lateral root emergence
Structure–function study of a Ca <sup>2+</sup> -independent metacaspase involved in lateral root emergence Open
Metacaspases are part of an evolutionarily broad family of multifunctional cysteine proteases, involved in disease and normal development. As the structure–function relationship of metacaspases remains poorly understood, we solved the X-ra…
View article: Thermoprotection by a cell membrane-localized metacaspase in a green alga
Thermoprotection by a cell membrane-localized metacaspase in a green alga Open
Caspases are restricted to animals, while other organisms, including plants possess metacaspases (MCAs), a more ancient and broader class of structurally-related yet biochemically distinct proteases. Our current understanding of plant MCAs…
View article: Variant Hyprocrea jecorina CBH1 cellulases
Variant Hyprocrea jecorina CBH1 cellulases Open
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
View article: Variant Hypocrea jercorina CBH1 cellulases
Variant Hypocrea jercorina CBH1 cellulases Open
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
View article: Variant Hypocrea jecorina CBH1 cellulases
Variant Hypocrea jecorina CBH1 cellulases Open
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
View article: Variant Hypocrea jecorina CBH1 cellulase
Variant Hypocrea jecorina CBH1 cellulase Open
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
View article: Structure-function analysis of a calcium-independent metacaspase reveals a novel proteolytic pathway for lateral root emergence
Structure-function analysis of a calcium-independent metacaspase reveals a novel proteolytic pathway for lateral root emergence Open
Metacaspases are part of an evolutionarily broad family of multifunctional cysteine proteases, involved in disease and normal development. Despite the extensive study of metacaspases in the two decades since their discovery, the structure-…
View article: Chiral recognition mechanism of cellobiohydrolase Cel7A for ligands based on the -blocker propranolol. The effect of explicit water molecules on binding and selectivities.
Chiral recognition mechanism of cellobiohydrolase Cel7A for ligands based on the -blocker propranolol. The effect of explicit water molecules on binding and selectivities. Open
: Proteins are useful chiral selectors. In order to understand the recognition mechanism and the chiral discrimination, binding of the (R)- and (S)-enantiomers of a series of designed amino alcohol inhibitors based on propranolol to cellob…
View article: Enzyme kinetics by <scp>GH7</scp> cellobiohydrolases on chromogenic substrates is dictated by non‐productive binding: insights from crystal structures and <scp>MD</scp> simulation
Enzyme kinetics by <span>GH7</span> cellobiohydrolases on chromogenic substrates is dictated by non‐productive binding: insights from crystal structures and <span>MD</span> simulation Open
Cellobiohydrolases (CBHs) in the glycoside hydrolase family 7 (GH7) ( EC3.2.1.176 ) are the major cellulose degrading enzymes both in industrial settings and in the context of carbon cycling in nature. Small carbohydrate conjugates such as…
View article: Glucomannan and beta-glucan degradation by Mytilus edulis Cel45A: Crystal structure and activity comparison with GH45 subfamily A, B and C
Glucomannan and beta-glucan degradation by Mytilus edulis Cel45A: Crystal structure and activity comparison with GH45 subfamily A, B and C Open
View article: Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases
Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases Open
View article: Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases
Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases Open
Family 7 glycoside hydrolases (GH7) are among the principal enzymes for cellulose degradation in nature and industrially. These important enzymes are often bimodular, comprised of a catalytic domain attached to a carbohydrate binding modul…
View article: Reply to Cosgrove: Non-enzymatic action of expansins
Reply to Cosgrove: Non-enzymatic action of expansins Open
In our computational study, we use molecular simulations to substantiate a hypothetical mechanism for glycosidic bond cleavage in the presence of a single catalytic acid at the active site of the mutant D10N HiCel45A. In addition to discus…
View article: Classification and Nomenclature of Metacaspases and Paracaspases: No More Confusion with Caspases
Classification and Nomenclature of Metacaspases and Paracaspases: No More Confusion with Caspases Open
View article: The hydrolysis mechanism of a GH45 cellulase and its potential relation to lytic transglycosylase and expansin function
The hydrolysis mechanism of a GH45 cellulase and its potential relation to lytic transglycosylase and expansin function Open
Family 45 glycoside hydrolases (GH45) are endoglucanases that are integral to cellulolytic secretomes, and their ability to break down cellulose has been successfully exploited in textile and detergent industries. In addition to their indu…
View article: The Dissociation Mechanism of the Processive Cellulase TrCel7A
The Dissociation Mechanism of the Processive Cellulase TrCel7A Open
View article: The dissociation mechanism of processive cellulases
The dissociation mechanism of processive cellulases Open
Cellulase enzymes deconstruct recalcitrant cellulose into soluble sugars, making them a biocatalyst of biotechnological interest for use in the nascent lignocellulosic bioeconomy. Cellobiohydrolases (CBHs) are cellulases capable of liberat…
View article: The dissociation mechanism of processive cellulases
The dissociation mechanism of processive cellulases Open
Cellulase enzymes deconstruct recalcitrant cellulose into soluble sugars, making them a biocatalyst of biotechnological interest for use in the nascent lignocellulosic bioeconomy. Cellobiohydrolases (CBHs) are cellulases capable of liberat…
View article: Side-by-side biochemical comparison of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare on their activity against crystalline cellulose and glucomannan
Side-by-side biochemical comparison of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare on their activity against crystalline cellulose and glucomannan Open
Our comparative studies reveal that the two lytic polysaccharide monooxygenases HiLPMO9B and HiLPMO9I from the white-rot conifer pathogen Heterobasidion irregulare display clear difference with respect to their activity against crystalline…
View article: Correlation of structure, function and protein dynamics in GH7 cellobiohydrolases from Trichoderma atroviride, T. reesei and T. harzianum
Correlation of structure, function and protein dynamics in GH7 cellobiohydrolases from Trichoderma atroviride, T. reesei and T. harzianum Open
We hypothesize that the combination of loop motifs is the main determinant for the observed differences in Cel7A activity on cellulosic substrates. Fine-tuning of the loop flexibility appears to be an important evolutionary target in Tr…
View article: Advantages of a distant cellulase catalytic base
Advantages of a distant cellulase catalytic base Open
The inverting glycoside hydrolase Trichoderma reesei (Hypocrea jecorina) Cel6A is a promising candidate for protein engineering for more economical production of biofuels. Until recently, its catalytic mechanism had been unce…
View article: Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes
Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes Open
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved in the synthesis, modification and degradation of complex carb…
View article: Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution
Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution Open
Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. H. jecorina Cel7A, cellobiohydrolase I, from glycoside hydrola…
View article: Biochemical and Structural Characterizations of Two Dictyostelium Cellobiohydrolases from the Amoebozoa Kingdom Reveal a High Level of Conservation between Distant Phylogenetic Trees of Life
Biochemical and Structural Characterizations of Two Dictyostelium Cellobiohydrolases from the Amoebozoa Kingdom Reveal a High Level of Conservation between Distant Phylogenetic Trees of Life Open
Glycoside hydrolase family 7 (GH7) cellobiohydrolases (CBHs) are enzymes commonly employed in plant cell wall degradation across eukaryotic kingdoms of life, as they provide significant hydrolytic potential in cellulose turnover. To date, …
View article: Who's on base? Revealing the catalytic mechanism of inverting family 6 glycoside hydrolases
Who's on base? Revealing the catalytic mechanism of inverting family 6 glycoside hydrolases Open
Unbiased simulations reveal a water wire enabling and rescuing the catalytic base of an inverting glycoside hydrolase.
View article: Sequencing, biochemical characterization, crystal structure and molecular dynamics of cellobiohydrolase Cel7A from <i>Geotrichum candidum</i> 3C
Sequencing, biochemical characterization, crystal structure and molecular dynamics of cellobiohydrolase Cel7A from <i>Geotrichum candidum</i> 3C Open
The ascomycete Geotrichum candidum is a versatile and efficient decay fungus that is involved, for example, in biodeterioration of compact discs; notably, the 3C strain was previously shown to degrade filter paper and cotton more efficient…