Explanipedia

IgE-activated human lung mast cells preferentially release prostaglandin D2, thromboxane A2, and cysteinyl-leukotrienes Open

Anna‐Karin Johnsson, Johan Kolmert, Elin Rönnberg, Jesper Säfholm, Mamdoh Al‐Ameri , et al. · 2025

There is a high degree of specialization in HLMC with a strong and specific increase in cysteinyl leukotrienes and PGD₂ but also the bronchoconstrictor thromboxane A₂ after IgE-dependent activation.

Allergic airway inflammation amplifies mast cell responses in isolated guinea pig intralobular bronchi Open

Mu Nie, Jielu Liu, Yujiao Xiang, Yiu Tung Anthony Wong, Emma Hendriks , et al. · 2025

A protocol to avoid postmortem constriction in isolated intralobular bronchi from guinea pigs was established. A new model makes it possible to investigate bronchial responses in airways with asthmatic features. Increased responses in bron…

Toll-like receptor activation induces airway obstruction and hyperresponsiveness in guinea pigs Open

Yujiao Xiang, Jielu Liu, Mu Nie, Gunnar Nilsson, Jesper Säfholm , et al. · 2024

Development of a Desorption Electrospray Ionization–Multiple-Reaction-Monitoring Mass Spectrometry (DESI-MRM) Workflow for Spatially Mapping Oxylipins in Pulmonary Tissue Open

Matthew J. Smith, Mu Nie, Mikael Adner, Jesper Säfholm, Craig E. Wheelock · 2024

Oxylipins are a class of low-abundance lipids formed via oxygenation of fatty acids. These compounds include potent signaling molecules (e.g., octadecanoids, eicosanoids) that can exert essential functions in the pathophysiology of inflamm…

Biomarkers for a less invasive strategy to predict children with eosinophilic esophagitis Open

Helena Thulin, Ladan Mansouri, Maria Altman, Simon Kebede Merid, Joachim Lundahl , et al. · 2024

Background Noninvasive biomarkers for diagnosing and monitoring eosinophilic esophagitis (EoE) are currently lacking. This study evaluates 20 biomarkers in serum and saliva, aiming to assess their diagnostic potential in pediatric EoE pati…

An optimized method for IgE-mediated degranulation of human lung mast cells Open

Yitao Gong, Anna‐Karin Johnsson, Jesper Säfholm, Mamdoh Al‐Ameri, Erik Sachs , et al. · 2024

Background Mast cells are critically involved in IgE-mediated diseases, e.g., allergies and asthma. Human mast cells are heterogeneous, and mast cells from different anatomical sites have been shown to respond differently to certain stimul…

Development of a desorption electrospray ionization –multiple-reaction-monitoring mass spectrometry (DESI-MRM) workflow for spatially mapping oxylipins in pulmonary tissue Open

Matthew J. Smith, Mu Nie, Mikael Adner, Jesper Säfholm, Craig E. Wheelock · 2024

Oxylipins are a class of low abundant lipids formed via oxygenation of fatty acids. These compounds include potent signaling molecules (e.g., octadecanoids, eicosanoids) that can exert essential functions in the pathophysiology of inflamma…

Granzyme B is elevated in esophageal biopsies in Pediatric Eosinophili Open

Helena Thulin, Jesper Säfholm, Joachim Lundahl, Viktor Jović, Mikael Adner , et al. · 2023

BACKGROUND: Eosinophilic esophagitis (EoE) is an immune-mediated antigen-triggered inflammatory disease of the esophagus. Our aim was to investigate inflammatory responses by a n

Accumulation of tissue-resident natural killer cells, innate lymphoid cells, and CD8 <sup>+</sup> T cells towards the center of human lung tumors Open

Demi Brownlie, Andreas von Kries, Giampiero Valenzano, Nicole Wild, Emel Yılmaz , et al. · 2023

Lung cancer is a leading cause of cancer-related death worldwide. Despite recent advances in tissue immunology, little is known about the spatial distribution of tissue-resident lymphocyte subsets in lung tumors. Using high-parameter flow …

Combined exposure to the alarmins TSLP, IL‐33 and IL‐25 enhances mast cell‐dependent contractions of human bronchi Open

Maria Belikova, Jesper Säfholm, Mamdoh Al‐Ameri, Ann‐Charlotte Orre, Sven‐Erik Dahlén , et al. · 2023

Asthma is a chronic inflammatory disease characterised by recurrent airflow obstruction where contraction of the smooth muscle is one main cause of the airway narrowing. Recent observations implicate thymic stromal lymphopoietin (TSLP), in…

Defining the contractile prostanoid component in hyperosmolar-induced bronchoconstriction in human small airways Open

Maria Belikova, Mamdoh Al‐Ameri, Ann‐Charlotte Orre, Jesper Säfholm · 2023

Exercise-induced bronchoconstriction (EIB) is thought to be triggered by increased osmolarity at the airway epithelium. The aim of this study was to define the contractile prostanoid component of EIB, using an ex vivo model where intact se…

Granzyme B is elevated in esophageal biopsies in Pediatric Eosinophilic Esophagitis Open

Helena Thulin, Jesper Säfholm, Joachim Lundahl, Viktor Jović, Mikael Adner , et al. · 2023

BACKGROUND: Eosinophilic esophagitis (EoE) is an immune-mediated antigen-triggered inflammatory disease of the esophagus. Our aim was to investigate inflammatory responses by a new ex vivo biopsy provocation-based method, stimulating biops…

Analysis of human lung mast cells by single cell RNA sequencing Open

Elin Rönnberg, Avinash Ravindran, Luca Mazzurana, Yitao Gong, Jesper Säfholm , et al. · 2023

Mast cells are tissue-resident cells playing major roles in homeostasis and disease conditions. Lung mast cells are particularly important in airway inflammatory diseases such as asthma. Human mast cells are classically divided into the su…

Monensin inhibits mast cell mediated airway contractions in human and guinea pig asthma models Open

Jielu Liu, Mu Nie, Caijuan Dong, Jesper Säfholm, Gunnar Pejler , et al. · 2022

Asthma is a common respiratory disease associated with airway hyperresponsiveness (AHR), airway inflammation and mast cell (MC) accumulation in the lung. Monensin, an ionophoric antibiotic, has been shown to induce apoptosis of human MCs. …

Cysteinyl-maresin 3 inhibits IL-13 induced airway hyperresponsiveness through alternative activation of the CysLT1 receptor Open

Jesper Säfholm, Willem Abma, Lora G. Bankova, Joshua A. Boyce, Mamdoh Al‐Ameri , et al. · 2022

MCTR3, but not LTD₄, decreased the IL-13-induced airway hyperresponsiveness by activation of the CysLT₁ receptor. The distinct actions of the two lipid mediators on the CysLT₁ receptor suggest an alternativ…

Activation of succinate receptor 1 boosts human mast cell reactivity and allergic bronchoconstriction Open

Xiao Tang, Elin Rönnberg, Jesper Säfholm, Madhuranayaki Thulasingam, Mette Trauelsen , et al. · 2022

Background SUCNR1 is a sensor of extracellular succinate, a Krebs cycle intermediate generated in excess during oxidative stress and has been linked to metabolic regulation and inflammation. While mast cells express SUCNR1, its role in mas…

Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity Open

Elin Rönnberg, Daryl Boey, Avinash Ravindran, Jesper Säfholm, Ann‐Charlotte Orre , et al. · 2022

Background Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensiv…

Complex Involvement of Interleukin-26 in Bacterial Lung Infection Open

F. Karlhans, Magnus Paulsson, Krzysztof Piersiala, Jakob Sax, Ibrahim Mboob , et al. · 2021

Pneumonia is a global cause of mortality, and this provides a strong incentive to improve the mechanistic understanding of innate immune responses in the lungs. Here, we characterized the involvement of the cytokine interleukin (IL)-26 in …

Immunoprofiling reveals novel mast cell receptors and the continuous nature of human lung mast cell heterogeneity Open

Elin Rönnberg, Daryl Boey Zhong Hao, Avinash Ravindran, Jesper Säfholm, Ann‐Charlotte Orre , et al. · 2021

Background: Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensi…

Distinct effects of antigen and compound 48/80 in the guinea pig trachea Open

Jielu Liu, Johan Kolmert, Jesper Säfholm, Anna‐Karin Johnsson, Javier Zurita , et al. · 2021

Mast cells (MCs) can be activated immunologically by allergen and non-immunologically via the Mas-related G protein-coupled receptor member X2 (MRGPRX2).1 With the emerging interest of MRGPRX2, we compared the airway smooth muscle (ASM) re…

Tissue-specific transcriptional imprinting and heterogeneity in human innate lymphoid cells revealed by full-length single-cell RNA-sequencing Open

Luca Mazzurana, Paulo Czarnewski, Viktor Jönsson, Leif Wigge, Markus Ringnér , et al. · 2021

Lipoxin A4 reduces house dust mite and TNFα-induced hyperreactivity in the mouse trachea Open

Willem Abma, Malin Noreby, Craig E. Wheelock, Sven‐Erik Dahlén, Mikael Adner , et al. · 2020

Lipoxin A₄ (LXA₄) is considered a specialised pro-resolving mediator that decreases inflammation: however, pro-inflammatory effects have been described in the airways. Here, we investigated whether LXA₄ cou…

IL-13 and IL-4, but not IL-5 nor IL-17A, induce hyperresponsiveness in isolated human small airways Open

Martijn L. Manson, Jesper Säfholm, Anna James, Anna‐Karin Johnsson, Per Bergman , et al. · 2019

Wnt-3a Induces Cytokine Release in Human Mast Cells Open

Julia Tebroke, Joris E. Lieverse, Jesper Säfholm, Gunnar Schulte, Gunnar Nilsson , et al. · 2019

Mast cells are well known for their detrimental effects in allergies and asthma, and Wnt signaling has recently been implicated in asthma and other airway diseases. However, it is not known if or how Wnts affect human mast cells. Since Wnt…

Divergent Effects of Acute and Prolonged Interleukin 33 Exposure on Mast Cell IgE-Mediated Functions Open

Elin Rönnberg, Avan Ghaib, Carlos Ceriol, Mattias Enoksson, Michel Arock , et al. · 2019

Background: Epithelial cytokines, including IL-33 and Thymic stromal lymphopoietin (TSLP), have attracted interest because of their roles in chronic allergic inflammation-related conditions such as asthma. Mast cells are one of the …

Mannitol triggers mast cell–dependent contractions of human small bronchi and prostacyclin bronchoprotection Open

Jesper Säfholm, Martijn L. Manson, Johan Bood, Mamdoh Al‐Ameri, Ann‐Charlotte Orre , et al. · 2019

Cover Image Open

Sandra Ekstedt, Jesper Säfholm, Susanna Kumlien Georén, Lars‐Olaf Cardell · 2019

The cover image is based on the Original Article Dividing neutrophils in subsets reveals a significant role for activated neutrophils in the development of airway hyperreactivity by Sandra Ekstedt et al, https://doi.org/10.1111/cea.13311.

Divergent effects of acute and prolonged interleukin 33 exposure on mast cell IgE-mediated functions Open

Elin Rönnberg, Avan Ghaib, Carlos Ceriol, Mattias Enoksson, Michel Arock , et al. · 2018

Background Epithelial cytokines, including IL-33 and TSLP, have attracted interest because of their roles in chronic allergic inflammation-related conditions such as asthma. Mast cells are one of the major targets of IL-33, to which they r…

An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells Open

Avinash Ravindran, Elin Rönnberg, Joakim S. Dahlin, Luca Mazzurana, Jesper Säfholm , et al. · 2018

Background: Mast cells are tissue-resident inflammatory cells defined by their high granularity and surface expression of the high-affinity IgE receptor, FcεRI, and CD117/KIT, the receptor for stem cell factor (SCF). There is a cons…

Prostaglandin E2 inhibits mast cell–dependent bronchoconstriction in human small airways through the E prostanoid subtype 2 receptor Open

Jesper Säfholm, Martijn L. Manson, Johan Bood, Ingrid Delin, Ann‐Charlotte Orre , et al. · 2015

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