Jesse D. Kirkpatrick
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View article: A 3D <i>in vitro</i> model of the human hepatobiliary junction
A 3D <i>in vitro</i> model of the human hepatobiliary junction Open
Cholestasis, or disruption in bile flow, is a common yet poorly understood feature of many liver diseases and injuries. Despite this, many engineered human tissue models of liver disease fail to recapitulate physiological bile flow. Here, …
View article: Multimodal profiling of proinflammatory protease activity identifies caspase-1 as a target for lung cancer interception
Multimodal profiling of proinflammatory protease activity identifies caspase-1 as a target for lung cancer interception Open
The systemic inhibition of IL-1b, a key mediator of pulmonary inflammation, has been shown to reduce the incidence of lung cancer in patients in years following treatment, but knowledge gaps surrounding its activation and role in the tumor…
View article: Engineering Multiplexed Synthetic Breath Biomarkers as Diagnostic Probes
Engineering Multiplexed Synthetic Breath Biomarkers as Diagnostic Probes Open
Breath biopsy is emerging as a rapid and non-invasive diagnostic tool that links exhaled chemical signatures with specific medical conditions. Despite its potential, clinical translation remains limited by the challenge of reliably detecti…
View article: Transposon-based oncogene integration in Abcb4(Mdr2)-/- mice recapitulates high susceptibility to cholangiocarcinoma in primary sclerosing cholangitis
Transposon-based oncogene integration in Abcb4(Mdr2)-/- mice recapitulates high susceptibility to cholangiocarcinoma in primary sclerosing cholangitis Open
Animal models for primary sclerosing cholangitis (PSC)-related cholangiocarcinoma (PSC-CCA) are lacking. Thus, we have developed and characterized a new mouse model of PSC-CCA, termed SB CCA.Mdr2-/-, which features reliable tumor induction…
View article: Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies
Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies Open
Liquid biopsies enable early detection and monitoring of diseases such as cancer, but their sensitivity remains limited by the scarcity of analytes such as cell-free DNA (cfDNA) in blood. Improvements to sensitivity have primarily relied o…
View article: Inhalable point-of-care urinary diagnostic platform
Inhalable point-of-care urinary diagnostic platform Open
Although low-dose computed tomography screening improves lung cancer survival in at-risk groups, inequality remains in lung cancer diagnosis due to limited access to and high costs of medical imaging infrastructure. We designed a needleles…
View article: Inhalable point-of-care urinary diagnostic platform
Inhalable point-of-care urinary diagnostic platform Open
The late-stage detection of lung cancer leads to a high global mortality rate. Although low-dose computed tomography screening improves lung cancer survival in at-risk groups, this test still suffers from high rates of false positive resul…
View article: Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer
Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer Open
Recent years have seen the emergence of conditionally activated diagnostics and therapeutics that leverage protease-cleavable peptide linkers to enhance their specificity for cancer. However, due to a lack of methods to measure and localiz…
View article: Supplementary Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer
Supplementary Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer Open
Complete Supplementary Information for the manuscript, including Supplemental Figures 1-16 and tables 1-4.
View article: Supplementary Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer
Supplementary Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer Open
Complete Supplementary Information for the manuscript, including Supplemental Figures 1-16 and tables 1-4.
View article: Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer
Data from Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer Open
Recent years have seen the emergence of conditionally activated diagnostics and therapeutics that leverage protease-cleavable peptide linkers to enhance their specificity for cancer. However, due to a lack of methods to measure and localiz…
View article: Low protease activity in B cell follicles promotes retention of intact antigens after immunization
Low protease activity in B cell follicles promotes retention of intact antigens after immunization Open
The structural integrity of vaccine antigens is critical to the generation of protective antibody responses, but the impact of protease activity on vaccination in vivo is poorly understood. We characterized protease activity in lymph nodes…
View article: A nanoparticle priming agent reduces cellular uptake of cell-free DNA and enhances the sensitivity of liquid biopsies
A nanoparticle priming agent reduces cellular uptake of cell-free DNA and enhances the sensitivity of liquid biopsies Open
Liquid biopsies are enabling minimally invasive monitoring and molecular profiling of diseases across medicine, but their sensitivity remains limited by the scarcity of cell-free DNA (cfDNA) in blood. Here, we report an intravenous priming…
View article: Multiscale profiling of protease activity in cancer
Multiscale profiling of protease activity in cancer Open
Diverse processes in cancer are mediated by enzymes, which most proximally exert their function through their activity. High-fidelity methods to profile enzyme activity are therefore critical to understanding and targeting the pathological…
View article: Multiscale profiling of protease activity in cancer
Multiscale profiling of protease activity in cancer Open
Diverse processes in cancer are mediated by enzymes, which most proximally exert their function through their activity. High-fidelity methods to profile enzyme activity are therefore critical to understanding and targeting the pathological…
View article: Ionic Liquid‐Mediated Transdermal Delivery of Thrombosis‐Detecting Nanosensors
Ionic Liquid‐Mediated Transdermal Delivery of Thrombosis‐Detecting Nanosensors Open
Blood clotting disorders such as pulmonary embolism are associated with high morbidity and mortality. A large portion of thrombotic events occur postoperative and after hospital discharge. Therefore, easily applicable, noninvasive, and lon…
View article: Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens
Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens Open
SUMMARY The structural integrity of vaccine antigens is critical, as antigen degradation in vivo could eliminate neutralizing epitopes and create competing B cell responses against irrelevant breakdown products. Using FRET imaging and imag…
View article: Multiscale profiling of enzyme activity in cancer
Multiscale profiling of enzyme activity in cancer Open
Diverse processes in cancer are mediated by enzymes, which most proximally exert their function through their activity. Methods to quantify enzyme activity, rather than just expression, are therefore critical to our ability to understand t…
View article: Mammalian meat allergy emerges after tick bite: the alpha-gal syndrome
Mammalian meat allergy emerges after tick bite: the alpha-gal syndrome Open
Alpha-gal syndrome (AGS) is a hypersensitivity reaction to mammalian meat that develops after tick bite exposure. AGS was first described in 2009 and testing for the allergy has become available in the last decade. We report the case of a …
View article: Protease activity sensors enable real-time treatment response monitoring in lymphangioleiomyomatosis
Protease activity sensors enable real-time treatment response monitoring in lymphangioleiomyomatosis Open
Background Biomarkers of disease progression and treatment response are urgently needed for patients with lymphangioleiomyomatosis (LAM). Activity-based nanosensors, an emerging biosensor class, detect dysregulated proteases in vivo and re…
View article: Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer
Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer Open
Recent years have seen the emergence of conditionally activated diagnostics and therapeutics that leverage protease-cleavable peptide linkers to enhance their specificity for cancer. However, due to a lack of methods to measure and localiz…
View article: Activatable zymography probes enable in situ localization of protease dysregulation in cancer
Activatable zymography probes enable in situ localization of protease dysregulation in cancer Open
Recent years have seen the emergence of conditionally activated diagnostics and therapeutics that leverage protease-cleavable peptide linkers to enhance their specificity for cancer. However, due to a lack of methods to measure and localiz…
View article: Urinary detection of lung cancer in mice via noninvasive pulmonary protease profiling
Urinary detection of lung cancer in mice via noninvasive pulmonary protease profiling Open
Intrapulmonary protease-sensitive nanoparticles detect lung cancer as a urinary readout in two genetically engineered mouse models.
View article: Classification of prostate cancer using a protease activity nanosensor library
Classification of prostate cancer using a protease activity nanosensor library Open
Significance Prostate cancer is the most common noncutaneous cancer in men, but there is a need for better biomarkers that can identify aggressive disease. Here, we describe a bottom-up approach to design nanosensors to detect and classify…
View article: A Size-Selective Intracellular Delivery Platform
A Size-Selective Intracellular Delivery Platform Open
Identifying and separating a subpopulation of cells from a heterogeneous mixture are essential elements of biological research. Current approaches require detailed knowledge of unique cell surface properties of the target cell population. …
View article: A Size‐Selective Intracellular Delivery Platform
A Size‐Selective Intracellular Delivery Platform Open
Identifying and separating a subpopulation of cells from a heterogeneous mixture are essential elements of biological research. Current approaches require detailed knowledge of unique cell surface properties of the target cell population. …