Jesse Aaron
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View article: Redefining colocalization analysis with a novel Phasor Mixing Coefficient
Redefining colocalization analysis with a novel Phasor Mixing Coefficient Open
The first step to probing any potential interaction between two biomolecules is to determine their spatial association. In other words, if two biomolecules localize similarly within a cell, then it is plausible they could interact. Traditi…
View article: A roadmap for the widespread adoption of frugal microscopes
A roadmap for the widespread adoption of frugal microscopes Open
Microscopy drives biological discovery, yet high costs limit its access to resource-limited regions. We highlight examples of successful frugal microscopes that have overcome adoption barriers, offering a roadmap to expand affordable, quan…
View article: De‐risking transformative microscopy technologies for broad adoption
De‐risking transformative microscopy technologies for broad adoption Open
The past 20 years have seen a paradigm‐shifting explosion of new optical microscopy technologies aimed at uncovering fundamental biological insights. Yet only a small portion ‘cross the finish line’ into wide adoption by the life science c…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Distinct mobility patterns of BRCA2 molecules at DNA damage sites Open
BRCA2 is an essential tumor suppressor protein involved in promoting faithful repair of DNA lesions. The activity of BRCA2 needs to be tuned precisely to be active when and where it is needed. Here, we quantified the spatio-temporal dynami…
View article: Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores
Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores Open
Single-molecule localization microscopy (SMLM) uses activatable or switchable fluorophores to create non-diffraction limited maps of molecular location in biological samples. Despite the utility of this imaging technique, the portfolio of …
View article: Focal adhesions contain three specialized actin nanoscale layers
Focal adhesions contain three specialized actin nanoscale layers Open
Focal adhesions (FAs) connect inner workings of cell to the extracellular matrix to control cell adhesion, migration and mechanosensing. Previous studies demonstrated that FAs contain three vertical layers, which connect extracellular matr…
View article: Four-dimensional quantitative analysis of cell plate development in Arabidopsis using lattice light sheet microscopy identifies robust transition points between growth phases
Four-dimensional quantitative analysis of cell plate development in Arabidopsis using lattice light sheet microscopy identifies robust transition points between growth phases Open
Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodelling, and timely deposition of polysaccharides, such as callose. Understanding such a dynam…
View article: More than just ‘added value’: The perils of not establishing shared core facilities in resource‐constrained communities
More than just ‘added value’: The perils of not establishing shared core facilities in resource‐constrained communities Open
The accelerating pace of technological advancements necessitates specialised expertise and cutting‐edge instruments to maintain competitive research in life sciences. Core facilities – collaborative laboratories equipped with state‐of‐the‐…
View article: Believing is seeing – the deceptive influence of bias in quantitative microscopy
Believing is seeing – the deceptive influence of bias in quantitative microscopy Open
The visual allure of microscopy makes it an intuitively powerful research tool. Intuition, however, can easily obscure or distort the reality of the information contained in an image. Common cognitive biases, combined with institutional pr…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2
Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2 Open
This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: single molecule tracking data of BRCA2-Hal…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2
Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2 Open
This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: single molecule tracking data of BRCA2-Hal…
View article: Imagining the future of optical microscopy: everything, everywhere, all at once
Imagining the future of optical microscopy: everything, everywhere, all at once Open
The optical microscope has revolutionized biology since at least the 17 th Century. Since then, it has progressed from a largely observational tool to a powerful bioanalytical platform. However, realizing its full potential to study live s…
View article: Four-dimensional quantitative analysis of cell plate development using lattice light sheet microscopy identifies robust transition points between growth phases
Four-dimensional quantitative analysis of cell plate development using lattice light sheet microscopy identifies robust transition points between growth phases Open
Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodeling, and timely polysaccharide deposition, such as callose. Such a dynamic process requires…
View article: Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome
Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome Open
A DNA damage-inducible mutagenic gene cassette has been implicated in the emergence of drug resistance in Mycobacterium tuberculosis during anti-tuberculosis (TB) chemotherapy. However, the molecular composition and operation of the encode…
View article: Dissecting Cell Plate Development During Plant Cytokinesis
Dissecting Cell Plate Development During Plant Cytokinesis Open
Journal Article Dissecting Cell Plate Development During Plant Cytokinesis Get access Rosalie Sinclair, Rosalie Sinclair University of California, Davis, United States Search for other works by this author on: Oxford Academic Google Schola…
View article: Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription
Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription Open
NDP52 is an autophagy receptor involved in the recognition and degradation of invading pathogens and damaged organelles. Although NDP52 was first identified in the nucleus and is expressed throughout the cell, to date, there is no clear nu…
View article: Author response: Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome
Author response: Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract A DNA d…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Distinct mobility patterns of BRCA2 molecules at DNA damage sites Open
BRCA2 is an essential tumor suppressor protein involved in promoting faithful repair of DNA lesions. The activity of BRCA2 needs to be tuned precisely to be active when and where it is needed. Here, we quantified the spatio-temporal dynami…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Distinct mobility patterns of BRCA2 molecules at DNA damage sites Open
This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: - Movies: This folder contains the movies …
View article: Specialized actin nanoscale layers control focal adhesion turnover
Specialized actin nanoscale layers control focal adhesion turnover Open
SUMMARY Focal adhesions (FAs) connect inner workings of the cell to the extracellular matrix to control cell adhesion, migration, and mechanosensing 1,2 . Previous studies demonstrated that FAs contain three vertical layers, which connect …
View article: Hyperspectral stimulated emission depletion microscopy and methods of use thereof
Hyperspectral stimulated emission depletion microscopy and methods of use thereof Open
A hyperspectral stimulated emission depletion ("STED") microscope system for high-resolution imaging of samples labeled with multiple fluorophores (e.g., two to ten fluorophores). The hyperspectral STED microscope includes a light source, …
View article: Single molecule microscopy to profile the effect of zinc status on transcription factor dynamics
Single molecule microscopy to profile the effect of zinc status on transcription factor dynamics Open
The regulation of transcription is a complex process that involves binding of transcription factors (TFs) to specific sequences, recruitment of cofactors and chromatin remodelers, assembly of the pre-initiation complex and recruitment of R…