Jesse Stombaugh
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View article: Minimal activation of the p53 DNA damage response by a modular cytosine base editor enables effective multiplexed gene knockout in induced pluripotent stem cells
Minimal activation of the p53 DNA damage response by a modular cytosine base editor enables effective multiplexed gene knockout in induced pluripotent stem cells Open
Precise genome editing of induced pluripotent stem cells (iPSC) holds great promise for engineering advanced cell therapies. CRISPR-Cas systems have been widely adopted in genome engineering applications, however their dependence on genoto…
View article: An aptamer-mediated base editing platform for simultaneous knock-in and multiple gene knockout for allogeneic CAR-T cells generation
An aptamer-mediated base editing platform for simultaneous knock-in and multiple gene knockout for allogeneic CAR-T cells generation Open
Gene editing technologies hold promise for enabling the next generation of adoptive cellular therapies. Conventional gene editing platforms that rely on nuclease activity, such as Clustered regularly interspaced short palindromic repeats-C…
View article: A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs
A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs Open
While CRISPR interference (CRISPRi) systems have been widely implemented in pooled lentiviral screening, there has been limited use with synthetic guide RNAs for the complex phenotypic readouts enabled by experiments in arrayed format. Her…
View article: Development and Characterization of a Modular CRISPR and RNA Aptamer Mediated Base Editing System
Development and Characterization of a Modular CRISPR and RNA Aptamer Mediated Base Editing System Open
Conventional CRISPR approaches for precision genome editing rely on the introduction of DNA double-strand breaks (DSB) and activation of homology-directed repair (HDR), which is inherently genotoxic and inefficient in somatic cells. The de…
View article: Microbiota Regulate Intestinal Absorption and Metabolism of Fatty Acids in the Zebrafish
Microbiota Regulate Intestinal Absorption and Metabolism of Fatty Acids in the Zebrafish Open
Regulation of intestinal dietary fat absorption is critical to maintaining energy balance. While intestinal microbiota clearly impact the host’s energy balance, their role in intestinal absorption and extra-intestinal metabolism of dietary…
View article: The Power Decoder Simulator for the Evaluation of Pooled shRNA Screen Performance
The Power Decoder Simulator for the Evaluation of Pooled shRNA Screen Performance Open
RNA interference screening using pooled, short hairpin RNA (shRNA) is a powerful, high-throughput tool for determining the biological relevance of genes for a phenotype. Assessing an shRNA pooled screen's performance is difficult in practi…