Jiahao Sha
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View article: Engineering neural recovery: Micro/nano-structured materials for nerve regeneration
Engineering neural recovery: Micro/nano-structured materials for nerve regeneration Open
View article: A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular Niche
A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular Niche Open
Childhood cancer treatments often impair male fertility due to gonadotoxic effects. While in vitro culture of prepubertal testis tissue offers a potential solution to preserve fertility, producing sufficient sperm remains a major barrier. …
View article: Aptamer/Dopamine Molecularly Imprinted Polymer Composite Based pH Stability Improved Electrochemical Sensor for Simultaneous Detection of Cd(Ⅱ) and Hg(Ⅱ)
Aptamer/Dopamine Molecularly Imprinted Polymer Composite Based pH Stability Improved Electrochemical Sensor for Simultaneous Detection of Cd(Ⅱ) and Hg(Ⅱ) Open
Cd(II) and Hg(II) are well‐known toxic heavy metal elements that are difficult to degrade by microorganisms in the environment. Limits for Cd(II) and Hg(II) in food have been established by the Chinese Government and other authorities. Apt…
View article: Drosophila melanogaster: A Model Organism in Muscular Dystrophy Studies
Drosophila melanogaster: A Model Organism in Muscular Dystrophy Studies Open
Muscular dystrophy is a group of complicated, genetically heterogeneous disorders characterized by progressive muscle weakness and degeneration. Due to the intricate nature, understanding the molecular mechanisms underlying muscular dystro…
View article: HumanTestisDB: A Comprehensive Atlas of Testicular Transcriptomes and Cellular Interactions
HumanTestisDB: A Comprehensive Atlas of Testicular Transcriptomes and Cellular Interactions Open
Advances in single-cell technology have enabled the detailed mapping of testicular cell transcriptomes, which is essential for understanding spermatogenesis. However, the fragmented nature of age-specific data from various literature sourc…
View article: Characterization of Metabolic Patterns in Mouse Spermatogenesis and Its Clinical Implications in Humans
Characterization of Metabolic Patterns in Mouse Spermatogenesis and Its Clinical Implications in Humans Open
Spermatogenesis is a complex process requiring precisely controlled metabolic adaptations. Although the genetic and cellular aspects of spermatogenesis have been extensively studied, the underlying metabolic mechanisms remain largely under…
View article: Stratified profiling of azoospermia: Differentiating histological subtypes with seminal plasma metabolic signatures
Stratified profiling of azoospermia: Differentiating histological subtypes with seminal plasma metabolic signatures Open
Non-obstructive azoospermia (NOA), characterized by impaired spermatogenesis and the complete absence of sperm in the ejaculate, represents one of the most severe forms of male infertility. Current diagnostic strategies rely on invasive pr…
View article: Improving <i>in vitro</i> induction efficiency of human primordial germ cell-like cells using N2B27 or NAC-based medium
Improving <i>in vitro</i> induction efficiency of human primordial germ cell-like cells using N2B27 or NAC-based medium Open
Primordial germ cells (PGCs), the precursors of oocytes or spermatozoa, are highly pluripotent. In recent years, the in vitro induction of human primordial germ cell-like cells (hPGCLCs) has advanced significantly. However, the stab…
View article: HumanTestisDB: A Comprehensive Atlas of Testicular Transcriptomics and Cellular Interactions
HumanTestisDB: A Comprehensive Atlas of Testicular Transcriptomics and Cellular Interactions Open
Advances in single-cell technology have enabled the detailed mapping of testicular cell transcriptomes, which is essential for understanding spermatogenesis. However, the fragmented nature of age-specific data from various literature sourc…
View article: Establishment of a novel non-integrated human pluripotent stem cell-based gastruloid model
Establishment of a novel non-integrated human pluripotent stem cell-based gastruloid model Open
Embryo loss and pregnancy disorders are prevalent worldwide, with both conditions critically associated with dysfunctioning gastrulation processes. Gastrulation and post-gastrulation organogenesis are crucial stages of embryonic developmen…
View article: Germ cell-specific deletion of <i>Pex3</i> reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis
Germ cell-specific deletion of <i>Pex3</i> reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis Open
Peroxisomes are organelles enclosed by a single membrane and are present in various species. The abruption of peroxisomes is correlated with peroxisome biogenesis disorders and single peroxisomal enzyme deficiencies that induce diverse dis…
View article: A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis
A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis Open
View article: A prime editor efficiently repaired human induced pluripotent stem cells with AR gene mutation (c.2710G > A; p. V904M)
A prime editor efficiently repaired human induced pluripotent stem cells with AR gene mutation (c.2710G > A; p. V904M) Open
Prime Editor (PE) is a precise genome manipulation technology based on the CRISPR-Cas9 system, while its application in human induced pluripotent stem cells (iPSCs) remains limited. Here, we established a repaired hiPS cell line (SKLRMi001…
View article: Derivation of new pluripotent stem cells from human extended pluripotent stem cells with formative features and trophectoderm potential
Derivation of new pluripotent stem cells from human extended pluripotent stem cells with formative features and trophectoderm potential Open
Previous studies have demonstrated the existence of intermediate stem cells, which have been successfully obtained from human naive pluripotent stem cells (PSCs) and peri‐implantation embryos. However, it is not known whether human extende…
View article: Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro
Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro Open
Epigenetic regulation, particularly post-translational modifications (PTMs) of histones, participates in spermatogonial stem cell (SSCs) differentiation. However, there is a lack of systemic studies of histone PTM regulation during the dif…
View article: Biallelic mutations in RNA-binding protein ADAD2 cause spermiogenic failure and non-obstructive azoospermia in humans
Biallelic mutations in RNA-binding protein ADAD2 cause spermiogenic failure and non-obstructive azoospermia in humans Open
STUDY QUESTION What are some pathogenic mutations for non-obstructive azoospermia (NOA) and their effects on spermatogenesis? SUMMARY ANSWER Biallelic missense and frameshift mutations in ADAD2 disrupt the differentiation of round spermati…
View article: Additional file 2 of A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis
Additional file 2 of A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis Open
Additional file 2: Table S1. Results of analysis of circRNA sequencing.
View article: Additional file 5 of A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis
Additional file 5 of A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis Open
Additional file 5. Individual data values.
View article: Functional screening of congenital heart disease risk loci identifies 5 genes essential for heart development in zebrafish
Functional screening of congenital heart disease risk loci identifies 5 genes essential for heart development in zebrafish Open
View article: Single-Cell Quantitative Proteomic Analysis of Human Oocyte Maturation Revealed High Heterogeneity in In Vitro–Matured Oocytes
Single-Cell Quantitative Proteomic Analysis of Human Oocyte Maturation Revealed High Heterogeneity in In Vitro–Matured Oocytes Open
Oocyte maturation is pertinent to the success of in vitro maturation (IVM), which is used to overcome female infertility, and produced over 5000 live births worldwide. However, the quality of human IVM oocytes has not been investigated at …
View article: Mebendazole-Induced Blood-Testis Barrier Injury in Mice Testes by Disrupting Microtubules in Addition to Triggering Programmed Cell Death
Mebendazole-Induced Blood-Testis Barrier Injury in Mice Testes by Disrupting Microtubules in Addition to Triggering Programmed Cell Death Open
Mebendazole (MBZ) is a synthetic benzimidazole known for its antiparasitic properties. In recent years, growing evidence showed that MBZ was also used as an anti-tumor agent. However, whether (and to what extent) this drug treatment affect…
View article: Single‐cell RNA‐Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation
Single‐cell RNA‐Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation Open
The assembly of primordial follicles in mammals represents one of the most critical processes in ovarian biology. It directly affects the number of oocytes available to a female throughout her reproductive life. Premature depletion of prim…
View article: Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study
Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study Open
View article: Peer Review #1 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.2)"
Peer Review #1 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.2)" Open
Background:The CCT complex is an important mediator of microtubule assembly and intracellular protein folding.Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis.In the present report, we therefore evaluat…
View article: Peer Review #1 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.1)"
Peer Review #1 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.1)" Open
Background:The CCT complex is an important mediator of microtubule assembly and intracellular protein folding.Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis.In the present report, we therefore evaluat…
View article: Peer Review #3 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.1)"
Peer Review #3 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.1)" Open
Background:The CCT complex is an important mediator of microtubule assembly and intracellular protein folding.Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis.In the present report, we therefore evaluat…
View article: Peer Review #2 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.1)"
Peer Review #2 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.1)" Open
Background:The CCT complex is an important mediator of microtubule assembly and intracellular protein folding.Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis.In the present report, we therefore evaluat…
View article: T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia
T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia Open
Background The CCT complex is an important mediator of microtubule assembly and intracellular protein folding. Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis. In the present report, we therefore evalu…
View article: Peer Review #3 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.2)"
Peer Review #3 of "T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia (v0.2)" Open
Background:The CCT complex is an important mediator of microtubule assembly and intracellular protein folding.Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis.In the present report, we therefore evaluat…
View article: Quantitative proteomic biomarkers from extracellular vesicles of human seminal plasma in the differential diagnosis of azoospermia
Quantitative proteomic biomarkers from extracellular vesicles of human seminal plasma in the differential diagnosis of azoospermia Open
Dear Editor, Azoospermia, which accounts for about 10%–15% of infertile men, is generally classified as obstructive azoospermia (OA) due to male reproductive tract obstruction and nonobstructive azoospermia (NOA) due to testicular failure.…