Explanipedia

Programmable conjugative CRISPR interference targeting genotoxin in the gut Open

Jiahe Li, Brian Hamp, Hania Timek, Chengyuan Fang, Zongqi Wang , et al. · 2025

Among microbially derived metabolites that influence host disease, colibactin garners increasing attention for its roles in the rising incidence of early-onset colorectal cancer. Produced by pks⁺ Escherichia coli, colibactin is a potent ge…

Programmable conjugative CRISPR interference targeting genotoxin in the gut Open

Brian Hamp, Hania Timek, Chengyuan Fang, Zongqi Wang, Amber E Haley , et al. · 2025

Among microbially derived metabolites that influence host disease, colibactin garners increasing attention for its roles in the rising incidence of early-onset colorectal cancer. Produced by pks ⁺ Escherichia coli , colibactin is a potent …

Repurposing the bacterial surface display technology for drug delivery Open

Shaobo Yang, Mengdi Yang, Maria Jennings, Hania Timek, Amber E Haley , et al. · 2025

Bacteria have emerged as versatile platforms for therapeutic delivery, owing to their inherent adaptability, genetic tractability, and ability to interface with the human microbiome and immune system. This review explores the evolution of …

Cascade amplification of therapeutic payloads for cancer immunotherapy Open

Yingzhong Li, Jiayu Wu, Na Li, Maoqing Wu, Zongqi Wang , et al. · 2025

Achieving sufficient therapeutic payload delivery remains a significant challenge in gene therapy, particularly for cancer immunotherapy, where payload thresholds are critical for efficacy. To address this, we developed a Cascade Amplifica…

Correction: Identification of a Fusobacterial RNA-binding protein involved in host small RNA-mediated growth inhibition Open

Pu‐Ting Dong, Mengdi Yang, Jie Hu, Lujia Cen, Peng Zhou , et al. · 2025

Effect of immune infiltration intensity on the efficacy of neoadjuvant immunotherapy for esophageal cancer Open

Yong Zhang, Xinyao Xu, Xiaorong Mu, Juzheng Wang, Jipeng Zhang , et al. · 2025

Background Esophageal squamous cell carcinoma (ESCC) treatment often involves neoadjuvant therapy combining chemotherapy and immune checkpoint inhibitors. However, the effectiveness of these treatments is limited by immune infiltration in …

Identification of a Fusobacterial RNA-binding protein involved in host small RNA-mediated growth inhibition Open

Pu‐Ting Dong, Mengdi Yang, Jie Hu, Lujia Cen, Peng Zhou , et al. · 2025

Host-derived small RNAs are emerging as critical regulators in the dynamic interactions between host tissues and the microbiome, with implications for microbial pathogenesis and host defense. Among these, transfer RNA-derived small RNAs (t…

The promise of synthetic bacteria in cancer immunotherapy: Revitalizing tumor immunity via IL-10R modulation Open

Jiahe Li, Allen P. Liu · 2025

Design of circularly polarized phosphorescence materials guided by transfer learning Open

Xu Liu, Yihan Zhang, Yifan Xie, L. L. Wang, Liyu Gan , et al. · 2025

It is highly desirable that artificial circularly polarized phosphorescent materials with high luminescence asymmetry factor (g_lum), narrowband emission and tunable chiral phosphorescent performance can be constructed. Especiall…

Physiologically relevant coculture model for oral microbial-host interactions Open

Zeyang Pang, Nicole Cady, Lujia Cen, Thomas M. Schmidt, Xuesong He , et al. · 2025

Engineering tripartite gene editing machinery for highly efficient non-viral targeted genome integration Open

Hangu Nam, Keqiang Xie, Ishita Majumdar, Jiao Wang, Shaobo Yang , et al. · 2025

Non-viral DNA donor templates are commonly used for targeted genomic integration via homologous recombination (HR), with efficiency improved by CRISPR/Cas9 technology. Circular single-stranded DNA (cssDNA) has been used as a genome enginee…

Physiologically Relevant Coculture Model for Oral Microbial-Host Interactions Open

Zeyang Pang, Nicole Cady, Lujia Cen, Thomas M. Schmidt, Xuesong He , et al. · 2025

Understanding microbial-host interactions in the oral cavity is essential for elucidating oral disease pathogenesis and its systemic implications. In vitro bacteria-host cell coculture models have enabled fundamental studies to characteriz…

In situ neutralization of the genotoxicity of pathogenic gut bacteria with the polyketide synthase island Open

Jiahe Li, Shaobo Yang, Zongqi Wang, Chengyuan Fang, Minmin Yang , et al. · 2024

The gut microbiota produce beneficial and deleterious metabolites to influence host physiology, with colibactin from pks⁺ bacteria one of the most studied metabolites associated with host genotoxicity and tumorigenesis. However,…

Construction of Energy Consumption Model in Asphalt Mixture Production Stage Based on Field Measurements Open

Chunhong Wang, Yubin Zhang, Xiaoli Fang, Hu Tao, Zhi Suo , et al. · 2024

To better construct an energy consumption model for the asphalt mixture production stage, this study divides the production process into four phases: aggregate dust removal, aggregate drying, asphalt heating, and mixture blending. Utilizin…

The mechanism of MMP14-positive tumor-associated fibroblast subsets in inhibiting PD-1 immunotherapy for esophageal cancer through exosomal tsRNA-10522 Open

Juzheng Wang, Chunlong Zheng, Jiayu Lu, Xinyao Xu, Guangyu Xiang , et al. · 2024

An autoantibody profile identified by human genome‐wide protein arrays in rheumatoid arthritis Open

Xu Liu, Xiaoying Zhang, Yu‐Jian Kang, Fei Huang, Shuang Liu , et al. · 2024

Precise diagnostic biomarkers of anticitrullination protein antibody (ACPA)‐negative and early‐stage RA are still to be improved. We aimed to screen autoantibodies in ACPA‐negative patients and evaluated their diagnostic performance. The h…

Identification of P-type ATPase as a bacterial transporter for host-derived small RNA Open

Pu‐Ting Dong, Mengdi Yang, Lujia Cen, Peng Zhou, Difei Xu , et al. · 2024

Host-derived small RNAs represent a finely tuned host response to maintain the host-microbial homeostasis. Among these, an emerging class known as transfer RNA-derived small RNA (tsRNA) has been implicated in modulating microbial-host inte…

LC-MS/MS-based chemical profiling of water extracts of Moringa oleifera leaves and pharmacokinetics of their major constituents in rat plasma Open

Jiahong Wang, Yiyang Du, Li Jiang, Jiahe Li, Bing Yu , et al. · 2024

Moringa oleifera leaves (MOL) are native to India and have high biological activities. To better understand the basic pharmacodynamic materials, the chemical components in MOL and their pharmacokinetic properties were studied and qu…

Nonpathogenic E. coli engineered to surface display cytokines as a new platform for immunotherapy Open

Rizwan Romee, Shaobo Yang, Michal Sheffer, Isabel Kaplan, Zongqi Wang , et al. · 2024

FAM20A is a golgi-localized Type II transmembrane protein Open

Mohd Faizan Siddiqui, Jiahe Li, Shuzhen Wang, Hua Zhang, Chunlin Qin , et al. · 2024

Family with sequence similarity 20, member A (FAM20A) is a pseudo-kinase in the secretory pathway and is essential for enamel formation in humans. Here we examine if FAM20A is a membrane-associated protein. We show that the full-length FAM…

Constitutive expression of spliced X-box binding protein 1 inhibits dentin formation in mice Open

Qian Xu, Jiahe Li, Hua Zhang, Shuzhen Wang, Chunlin Qin , et al. · 2024

Upon endoplasmic reticulum (ER) stress, inositol-requiring enzyme 1 (IRE1) is activated, which subsequently converts an unspliced X-box binding protein 1 ( XBP1U ) mRNA to a spliced mRNA that encodes a potent XBP1S transcription factor. XB…

Engineered<i>Bacillus subtilis</i>as oral probiotics to enhance clearance of blood lactate Open

Mengdi Yang, Noah Hutchinson, Ningyuan Ye, Jianing Yin, Ming Guan , et al. · 2023

Elevated lactate concentrations are implicated in various acute and chronic diseases such as sepsis and mitochondrial dysfunction, respectively. Conversely, ineffective lactate clearance is associated with poor clinical prognoses and high …

Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration Open

Hao Wu, Hangu Nam, Keqiang Xie, Ishita Majumdar, Shaobo Yang , et al. · 2023

TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration Open

Hangu Nam, Keqiang Xie, Ishita Majumdar, Shaobo Yang, Jakob Starzyk , et al. · 2023

Summary/Abstract Non-viral DNA donor template has been widely used for targeted genomic integration by homologous recombination (HR). This process has become more efficient with RNA guided endonuclease editor system such as CRISPR/Cas9. Ci…

Revolutionizing Infection Risk Scoring: An Ensemble “From Weak to Strong” Deduction Strategy and Enhanced Point‐of‐Care Testing Tools Open

Haoman Chen, Hui Ye, Fanxuan Chen, Yi Shi, Jiahe Li , et al. · 2023

The COVID‐19 pandemic exacerbates challenges faced by human immunodeficiency virus patients, who are at heightened risk for infection due to compromised immune systems. This study aims to develop a reliable, home‐based point‐of‐care testin…

3D Printing of Interpenetrating Network Flexible Hydrogels with Enhancement of Adhesiveness Open

Lei Zhang, Huifeng Du, Xin Sun, Cheng Feng, Wenhan Lee , et al. · 2023

3D printing of hydrogels has been widely explored for the rapid fabrication of complex soft structures and devices. However, using 3D printing to customize hydrogels with both adequate adhesiveness and toughness remains a fundamental chall…

Amplification and Dynamic Control of Circularly Polarized Luminescence in Poly (vinyl Alcohol)/CdSe@CdS Nanorod Assembly Twisted‐Stacking Hetero‐Structures Open

Wen Li, Yifan Xie, Peng Yang, Hongli Zhang, Jiahe Li , et al. · 2023

Chiroptical materials with strong and switchable circularly polarized luminescence (CPL) properties have gained considerable interest due to their feasible applications in optoelectronic devices, sensing, and information encryption. Howeve…

Novel, accurate pathogen sensors for fast detection of SARS-CoV-2 in the aerosol in seconds for a breathalyzer platform Open

Xiaoling Shi, Pardis Sadeghi, Nader Lobandi, Shadi Emam, Seyed Mahdi Seyed Abrishami , et al. · 2023

Rapid and accurate detection of the pathogens, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for COVID-19, is critical for mitigating the COVID-19 pandemic. Current state-of-the-art pathogen tests for COVID-19 diagno…

Targeting <i>Fusobacterium nucleatum</i> through chemical modifications of host-derived transfer RNA fragments Open

Mengdi Yang, Pu‐Ting Dong, Lujia Cen, Wenyuan Shi, Xuesong He , et al. · 2023

Host mucosal barriers possess an arsenal of defense molecules to maintain host-microbe homeostasis such as antimicrobial peptides and immunoglobulins. In addition to these well-established defense molecules, we recently reported small RNAs…

Supplementary Data from Phenotypic Heterogeneity and Metastasis of Breast Cancer Cells Open

Lauren A. Hapach, Shawn P. Carey, Samantha C. Schwager, Paul Taufalele, Wenjun Wang , et al. · 2023

Supplemental data and methods

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