Jihyeon Yu
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View article: Neuronal mitochondrial disaggregase CLPB ameliorates Huntington's disease pathology in mice
Neuronal mitochondrial disaggregase CLPB ameliorates Huntington's disease pathology in mice Open
View article: Identification of optimal adenine and cytosine base editors for genome editing in <i>Arabidopsis</i> and soybean
Identification of optimal adenine and cytosine base editors for genome editing in <i>Arabidopsis</i> and soybean Open
Base editors, including adenine base editors (ABEs) and cytosine base editors (CBEs), are widely used in numerous organisms to introduce site-specific sequence modifications in genomic DNA without causing double-strand breaks (DSBs). Howev…
View article: Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean.
Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean. Open
Base editors, including adenine base editors (ABEs) and cytosine base editors (CBEs), are widely used in numerous organisms to introduce site-specific sequence modifications in genomic DNA without causing double-strand breaks (DSBs). Howev…
View article: Autophagy induction enhances homologous recombination-associated CRISPR–Cas9 gene editing
Autophagy induction enhances homologous recombination-associated CRISPR–Cas9 gene editing Open
CRISPR (clustered regularly interspaced short palindromic repeats)–Cas9 (CRISPR-associated protein 9)-based gene editing via homologous recombination (HR) enables precise gene correction and insertion. However, its low efficiency poses a c…
View article: Impaired chaperone-mediated autophagy leads to abnormal SORT1 (sortilin 1) turnover and CES1-dependent triglyceride hydrolysis
Impaired chaperone-mediated autophagy leads to abnormal SORT1 (sortilin 1) turnover and CES1-dependent triglyceride hydrolysis Open
SORT1 (sortilin 1), a member of the the Vps10 (vacuolar protein sorting 10) family, is involved in hepatic lipid metabolism by regulating very low-density lipoprotein (VLDL) secretion and facilitating the lysosomal degradation of CES1 (car…
View article: Production of the antimalarial drug precursor amorphadiene by microbial terpene synthase-like from the moss Sanionia uncinata
Production of the antimalarial drug precursor amorphadiene by microbial terpene synthase-like from the moss Sanionia uncinata Open
View article: Biotin Contents of Grain Products Consumed in Korea
Biotin Contents of Grain Products Consumed in Korea Open
Recently, the consumption of processed foods has been increasing due to changes in lifestyle.However, little is known about the biotin content of these foods.In this study, the biotin content of 28 kinds of rice products, 29 kinds of wheat…
View article: Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice Open
Pre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) a…
View article: Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1
Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1 Open
View article: Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of OsOr Gene
Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of OsOr Gene Open
We generated an orange-colored (OC) rice callus line by targeted mutagenesis of the orange gene (OsOr) using the CRISPR-Cas9 system. The OC line accumulated more lutein, β-carotene, and two β-carotene isomers compared to the WT callus line…
View article: Transcriptome and Metabolite Profiling of Tomato SGR-Knockout Null Lines Using the CRISPR/Cas9 System
Transcriptome and Metabolite Profiling of Tomato SGR-Knockout Null Lines Using the CRISPR/Cas9 System Open
Stay-green 1 (SGR1) protein is a critical regulator of chlorophyll degradation and senescence in plant leaves; however, the functions of tomato SGR1 remain ambiguous. Here, we generated an SGR1-knockout (KO) null line via clustered regular…
View article: Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (LcyE) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice
Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (LcyE) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice Open
Lycopene epsilon-cyclase (LcyE) is a key enzyme in the carotenoid biosynthetic pathway of higher plants. Using the CRSPR/Cas9 and the geminiviral replicon, we optimized a method for targeted mutagenesis and golden SNP replacement of the Lc…
View article: Identification and Characterization of PSEUDO-RESPONSE REGULATOR (PRR) 1a and 1b Genes by CRISPR/Cas9-Targeted Mutagenesis in Chinese Cabbage (Brassica rapa L.)
Identification and Characterization of PSEUDO-RESPONSE REGULATOR (PRR) 1a and 1b Genes by CRISPR/Cas9-Targeted Mutagenesis in Chinese Cabbage (Brassica rapa L.) Open
The CRISPR/Cas9 site-directed gene-editing system offers great advantages for identifying gene function and crop improvement. The circadian clock measures and conveys day length information to control rhythmic hypocotyl growth in photoperi…
View article: Statistical optimization of phytol and polyunsaturated fatty acid production in the Antarctic microalga <italic>Micractinium variabile</italic> KSF0031
Statistical optimization of phytol and polyunsaturated fatty acid production in the Antarctic microalga Micractinium variabile KSF0031 Open
Polar microorganisms produce physiologically active substances to adapt to harsh environments, and these substances can be used as biomedical compounds. The green microalga Micractinium variabile KSF0031, which was isolated from Antarctica…
View article: The two clock proteins CCA1 and LHY activate <i>VIN3</i> transcription during vernalization through the vernalization-responsive cis-element
The two clock proteins CCA1 and LHY activate <i>VIN3</i> transcription during vernalization through the vernalization-responsive cis-element Open
Vernalization, a long-term cold-mediated acquisition of flowering competence, is critically regulated by VERNALIZATION INSENSITIVE 3 (VIN3), a gene induced by vernalization in Arabidopsis. Although the function of VIN3 has been extensively…
View article: Arabidopsis ATXR2 represses de novo shoot organogenesis in the transition from callus to shoot formation
Arabidopsis ATXR2 represses de novo shoot organogenesis in the transition from callus to shoot formation Open
Plants exhibit high regenerative capacity, which is controlled by various genetic factors. Here, we report that ARABIDOPSIS TRITHORAX-RELATED 2 (ATXR2) controls de novo shoot organogenesis by regulating auxin-cytokinin interaction. The aux…
View article: AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation
AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation Open
I-motif or C4 is a four-stranded DNA structure with a protonated cytosine:cytosine base pair (C+:C) found in cytosine-rich sequences. We have found that oligodeoxynucleotides containing adenine and cytosine repeats form a stable secondary …
View article: High-purity production and precise editing of DNA base editing ribonucleoproteins
High-purity production and precise editing of DNA base editing ribonucleoproteins Open
Cytosine and adenine base editor ribonucleoproteins show precise base editing with reduced DNA and RNA off-target effects.
View article: Analysis of NHEJ-Based DNA Repair after CRISPR-Mediated DNA Cleavage
Analysis of NHEJ-Based DNA Repair after CRISPR-Mediated DNA Cleavage Open
Genome editing using CRISPR-Cas9 nucleases is based on the repair of the DNA double-strand break (DSB). In eukaryotic cells, DSBs are rejoined through homology-directed repair (HDR), non-homologous end joining (NHEJ) or microhomology-media…
View article: In vivo gene editing via homology-independent targeted integration for adrenoleukodystrophy treatment
In vivo gene editing via homology-independent targeted integration for adrenoleukodystrophy treatment Open
View article: Coronavirus Antigens: Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens (Adv. Biology 4/2021)
Coronavirus Antigens: Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens (Adv. Biology 4/2021) Open
The production of biomolecules that require human‐specific lipid environments is extremely useful for basic research and medical applications. In article number 2000154, Seong‐Jun Kim, Jae‐Sung Woo, Sangsu Bae, and co‐workers integrate mul…
View article: Enhancing plant immunity by expression of pathogen-targeted CRISPR-Cas9 in plants
Enhancing plant immunity by expression of pathogen-targeted CRISPR-Cas9 in plants Open
Recent studies showed that CRISPR nucleases can boost plant immunity against infected virus by inducing the cleavage of viral dsDNA intermediate in a host plant. Here, we demonstrate that CRISPR-Cas9 can also improve plant resistance again…
View article: Mitochondrial genome of the Antarctic microalga <i>Micractinium simplicissimum</i> KSF0127 (Chlorellaceae, Trebouxiophyceae)
Mitochondrial genome of the Antarctic microalga <i>Micractinium simplicissimum</i> KSF0127 (Chlorellaceae, Trebouxiophyceae) Open
We report the first mitochondrial genome of the Antarctic microalga Micractinium simplicissimum KSF0127. The circular mitochondrial genome was 67,923 bp in length and contained 45 protein-coding genes, one ribosomal RNA gene, and 60…
View article: Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens
Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens Open
Coproduction of multiple proteins at high levels in a single human cell line would be extremely useful for basic research and medical applications. Here, a novel strategy for the stable expression of multiple proteins by integrating the ge…
View article: <i>Ex vivo</i>therapeutic base and prime editing using chemically derived hepatic progenitors in a mouse model of tyrosinemia type 1
<i>Ex vivo</i>therapeutic base and prime editing using chemically derived hepatic progenitors in a mouse model of tyrosinemia type 1 Open
Summary DNA base editors and prime editing technology capable of therapeutic base conversion enable ex vivo gene editing therapy for various genetic diseases. For such therapy, it is critical that the target cells survive well both outside…
View article: Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice
Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice Open
The rice SLR1 gene encodes the DELLA protein (protein with DELLA amino acid motif), and a loss-of-function mutation is dwarfed by inhibiting plant growth. We generate slr1-d mutants with a semi-dominant dwarf phenotype to target mutations …
View article: Purification of an Intact Human Protein Overexpressed from Its Endogenous Locus <i>via</i> Direct Genome Engineering
Purification of an Intact Human Protein Overexpressed from Its Endogenous Locus <i>via</i> Direct Genome Engineering Open
The overproduction and purification of human proteins is a requisite of both basic and medical research. Although many recombinant human proteins have been purified, current protein production methods have several limitations; recombinant …
View article: CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual
CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual Open
View article: <i>Ex Vivo</i> Therapeutic Base and Prime Editing Using Chemically Derived Hepatic Progenitors in a Mouse Model of Tyrosinemia Type 1
<i>Ex Vivo</i> Therapeutic Base and Prime Editing Using Chemically Derived Hepatic Progenitors in a Mouse Model of Tyrosinemia Type 1 Open
View article: CRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells
CRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells Open