Jiumei Hu
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View article: A Unified Multiplex Digital PCR-based Platform for Multi-class Biomarker Quantification from Low-volume Blood
A Unified Multiplex Digital PCR-based Platform for Multi-class Biomarker Quantification from Low-volume Blood Open
Simultaneous quantification of multiple biomarker classes from blood is essential for improving diagnostic accuracy, yet current methods remain limited by low multiplexing capacity, reliance on separate instruments and workflows, and incon…
View article: An unbiased approach to measure aberrant DNA methylation alterations
An unbiased approach to measure aberrant DNA methylation alterations Open
The ability to accurately measure aberrant DNA methylation levels, which occur in multiple diseases including cancer, is integral to the understanding of DNA methylation biology. It is well-established that in cancer, the largest, and thus…
View article: Multiplex digital profiling of DNA methylation heterogeneity for sensitive and cost-effective cancer detection in low-volume liquid biopsies
Multiplex digital profiling of DNA methylation heterogeneity for sensitive and cost-effective cancer detection in low-volume liquid biopsies Open
Molecular alterations in cancerous tissues exhibit intercellular genetic and epigenetic heterogeneity, complicating the performance of diagnostic assays, particularly for early cancer detection. Conventional liquid biopsy methods have limi…
View article: A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection
A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection Open
The detection of protein biomarkers presenting at low concentrations in biological fluids is essential for disease diagnosis and therapeutic monitoring. While magnetic beads-based solid-phase immunoassays have shown promise in achieving hi…
View article: Exploiting β‐Lactams‐Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of <i>Neisseria Gonorrhoeae</i> via Dual‐Digital PCR
Exploiting β‐Lactams‐Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of <i>Neisseria Gonorrhoeae</i> via Dual‐Digital PCR Open
The evolution of antimicrobial resistance (AMR) presents substantial challenges to global medical health systems. Neisseria gonorrhoeae ( N. gonorrhoeae ), in particular, has developed resistance to all currently available antimicrobials. …
View article: Enhanced CRISPR/Cas-Based Immunoassay through Magnetic Proximity Extension and Detection
Enhanced CRISPR/Cas-Based Immunoassay through Magnetic Proximity Extension and Detection Open
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-associated systems have recently emerged as a focal point for developing next-generation molecular diagnosis, particularly for nucleic acid detection. However, the dete…
View article: A Highly Sensitive Point-of-Care Covid-19 Serological Test using Immuno-PCR in 35 Mins
A Highly Sensitive Point-of-Care Covid-19 Serological Test using Immuno-PCR in 35 Mins Open
Serological tests play important roles in the fight against the Coronavirus Disease 2019 (COVID-19). The most commonly used methods, enzyme-linked immunosorbent assays (ELISA), provide reliable and sensitive antibody measurement though the…
View article: Unusual isothermal multimerization and amplification by the strand-displacing DNA polymerases with reverse transcription activities
Unusual isothermal multimerization and amplification by the strand-displacing DNA polymerases with reverse transcription activities Open
Abstarct Existing isothermal nucleic acid amplification (INAA) relying on the strand displacement activity of DNA polymerase usually requires at least two primers. However, in this paper, we report an unusual isothermal multimerization and…