Jennifer J. Hunter
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View article: Radiometric and safety assessment of a ‘low‐level red‐light’ myopia control device
Radiometric and safety assessment of a ‘low‐level red‐light’ myopia control device Open
Purpose Recent literature has demonstrated that ‘low‐level red‐light therapy’ may be effective at slowing axial elongation in children, but reports have questioned the safety of some red‐light devices. The current work explored the beam ch…
View article: A 3D iPSC retina model reveals non-cell-autonomous and non-neuronal mechanism of photoreceptor degeneration in a lysosomal storage disorder.
A 3D iPSC retina model reveals non-cell-autonomous and non-neuronal mechanism of photoreceptor degeneration in a lysosomal storage disorder. Open
Acid ceramidase deficiency and consequently altered sphingolipid signaling promotes disease phenotype(s) in a lysosomal storage disorder, CLN3 disease.
View article: Multispectral label-free in vivo cellular imaging of human retinal pigment epithelium using adaptive optics fluorescence lifetime ophthalmoscopy improves feasibility for low emission analysis and increases sensitivity for detecting changes with age and eccentricity
Multispectral label-free in vivo cellular imaging of human retinal pigment epithelium using adaptive optics fluorescence lifetime ophthalmoscopy improves feasibility for low emission analysis and increases sensitivity for detecting changes with age and eccentricity Open
Multispectral AOFLIO can provide a more comprehensive picture of changes with age and eccentricity. These results indicate that cell segmentation has the potential to allow investigations in low-photon scenarios such as in older or disease…
View article: Near Infrared Autofluorescence Lifetime Imaging of Human Retinal Pigment Epithelium Using Adaptive Optics Scanning Light Ophthalmoscopy
Near Infrared Autofluorescence Lifetime Imaging of Human Retinal Pigment Epithelium Using Adaptive Optics Scanning Light Ophthalmoscopy Open
NIR-AOFLIO was repeatable and allowed visualization of the RPE cellular mosaic. An observed signal in only the pigmented mouse extract infers the fluorescence signal originates predominantly from melanin. Variations observed across the ret…
View article: Fluorescence Lifetime Imaging of Human Retinal Pigment Epithelium in Pentosan Polysulfate Toxicity Using Adaptive Optics Scanning Light Ophthalmoscopy
Fluorescence Lifetime Imaging of Human Retinal Pigment Epithelium in Pentosan Polysulfate Toxicity Using Adaptive Optics Scanning Light Ophthalmoscopy Open
Our study shows evidence that AOFLIO can provide meaningful information in outer retinal disease beyond what is obtainable from fluorescence intensity alone. More studies are needed to determine the prognostic value of AOFLIO.
View article: Spectral Dependence of Light Exposure on Retinal Pigment Epithelium Disruption in Living Primate Retina
Spectral Dependence of Light Exposure on Retinal Pigment Epithelium Disruption in Living Primate Retina Open
We measured with FAOSLO the action spectrum dependence for photochemical RPE disruption across the visible light spectrum. Using this in vivo measure of phototoxicity provided by FAOSLO, we find that thresholds are lower than previously me…
View article: Analysis of amyloid deposits in the retina and implications for use as a biomarker of Alzheimer’s Disease
Analysis of amyloid deposits in the retina and implications for use as a biomarker of Alzheimer’s Disease Open
Background In humans and the naturally occurring canine model of Alzheimer’s disease (AD), we and others observe amyloid deposits in the retina en face. We have previously shown that detecting the number of amyloid deposits with polarised …
View article: Adaptive optics fluorescence lifetime imaging ophthalmoscopy of in vivo human retinal pigment epithelium: erratum
Adaptive optics fluorescence lifetime imaging ophthalmoscopy of in vivo human retinal pigment epithelium: erratum Open
This erratum corrects an error in Table 4 of our paper [ Biomed. Opt. Express 13 , 1737 ( 2022 ) 10.1364/BOE.451628 ].
View article: Separate lifetime signatures of macaque S cones, M/L cones, and rods observed with adaptive optics fluorescence lifetime ophthalmoscopy
Separate lifetime signatures of macaque S cones, M/L cones, and rods observed with adaptive optics fluorescence lifetime ophthalmoscopy Open
In the retina, several molecules involved in metabolism, the visual cycle, and other roles exhibit intrinsic fluorescence. The overall properties of retinal fluorescence depend on changes to the composition of these molecules and their env…
View article: Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution
Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution Open
Stem cell-based transplantation therapies offer hope for currently untreatable retinal degenerations; however, preclinical progress has been largely confined to rodent models. Here, we describe an experimental platform for accelerating pho…
View article: Localized Photoreceptor Ablation Using Femtosecond Pulses Focused With Adaptive Optics
Localized Photoreceptor Ablation Using Femtosecond Pulses Focused With Adaptive Optics Open
With this model, a variety of vision restoration methods can be tested in the non-human primate.
View article: Imaging Retinal Activity in the Living Eye
Imaging Retinal Activity in the Living Eye Open
Retinal function has long been studied with psychophysical methods in humans, whereas detailed functional studies of vision have been conducted mostly in animals owing to the invasive nature of physiological approaches. There are exception…
View article: Ultrafast laser induced retinal degeneration model in macaque using adaptive optics
Ultrafast laser induced retinal degeneration model in macaque using adaptive optics Open
The promise of vision restoration in human remains challenging due to the absence of a retinal degeneration model in non-human primates. There has been tremendous progress in vision restoration using stem cell therapy and optogenetics in r…
View article: Two photon ophthalmoscopy reveals a new oddity of S cones
Two photon ophthalmoscopy reveals a new oddity of S cones Open
Two-photon excited fluorescence (TPEF) ophthalmoscopy provides novel images of retinal structure and function, such as the first images of single ganglion cells and tracking intermediate stages of the visual cycle in the primate eye. TPEF …
View article: Selective S Cone Damage and Retinal Remodeling Following Intense Ultrashort Pulse Laser Exposures in the Near-Infrared
Selective S Cone Damage and Retinal Remodeling Following Intense Ultrashort Pulse Laser Exposures in the Near-Infrared Open
Interestingly, S cones are the receptor class with the lowest sensitivity in the near-infrared but are known to be particularly susceptible to ultraviolet and blue light. This effect of selective S cone damage after intense infrared ultras…
View article: Cellular-scale evaluation of induced photoreceptor degeneration in the living primate eye
Cellular-scale evaluation of induced photoreceptor degeneration in the living primate eye Open
Progress is needed in developing animal models of photoreceptor degeneration and evaluating such models with longitudinal, noninvasive techniques. We employ confocal scanning laser ophthalmoscopy, optical coherence tomography (OCT) and hig…
View article: Selective photoreceptor changes after ultrashort pulse laser exposure in the infrared
Selective photoreceptor changes after ultrashort pulse laser exposure in the infrared Open
For two-photon excited fluorescence (TPEF) retinal imaging, infrared (IR) ultrashort pulse lasers are used to excite fluorophores such as all-trans-retinol and NADH in the ultraviolet (UV), a spectral range that is usually blocked by the o…
View article: <i>In vivo</i> imaging of photoreceptor structure and function in a non-human primate model of retinal degeneration
<i>In vivo</i> imaging of photoreceptor structure and function in a non-human primate model of retinal degeneration Open
Here we employ adaptive optics scanning light ophthalmoscopy (AOSLO) to assess cellular-scale changes in photoreceptor structure and function in a non-human primate model of retinal degeneration. The retinas of four macaques were subretina…
View article: Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of exogenous fluorophores in mice
Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of exogenous fluorophores in mice Open
In vivo cellular scale fluorescence lifetime imaging of the mouse retina has the potential to be a sensitive marker of retinal cell health. In this study, we demonstrate fluorescence lifetime imaging of extrinsic fluorophores using adaptiv…
View article: Formation and Clearance of All- <i>Trans</i> -Retinol in Rods Investigated in the Living Primate Eye With Two-Photon Ophthalmoscopy
Formation and Clearance of All- <i>Trans</i> -Retinol in Rods Investigated in the Living Primate Eye With Two-Photon Ophthalmoscopy Open
This study demonstrates a method for rapidly measuring the rate of clearance of retinol in vivo. Moreover, TPEF generated due to retinol can be used as a measure of rhodopsin depletion, similar to densitometry. This enhances the utility of…
View article: Imaging individual neurons in the retinal ganglion cell layer of the living eye
Imaging individual neurons in the retinal ganglion cell layer of the living eye Open
Significance Retinal ganglion cells are the primary output neurons of the retina that process visual information and transmit it to the brain. We developed a method to reveal these cells in the living eye that does not require the fluoresc…
View article: Safety assessment in macaques of light exposures for functional two-photon ophthalmoscopy in humans
Safety assessment in macaques of light exposures for functional two-photon ophthalmoscopy in humans Open
Two-photon ophthalmoscopy has potential for in vivo assessment of function of normal and diseased retina. However, light safety of the sub-100 fs laser typically used is a major concern and safety standards are not well established. To tes…
View article: Two-Photon Autofluorescence Imaging Reveals Cellular Structures Throughout the Retina of the Living Primate Eye
Two-Photon Autofluorescence Imaging Reveals Cellular Structures Throughout the Retina of the Living Primate Eye Open
This in vivo survey of two-photon autofluorescence throughout the primate retina demonstrates a wider variety of structural detail in the living eye than is available through conventional imaging methods, and broadens the use of two-photon…
View article: In Vivo Two-Photon Fluorescence Kinetics of Primate Rods and Cones
In Vivo Two-Photon Fluorescence Kinetics of Primate Rods and Cones Open
Although other molecules could be contributing, we posit that these fluorescence changes are mediated by products of the retinoid cycle. In vivo two-photon ophthalmoscopy provides a way to monitor noninvasively stages of the retinoid cycle…