Joakim S. Dahlin
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View article: Single-cell omics-based characterization of human basophils reveals two transcriptionally distinct populations
Single-cell omics-based characterization of human basophils reveals two transcriptionally distinct populations Open
Background Basophils are implicated in various diseases including allergies, but a comprehensive characterization of human basophils has yet to be performed at the single-cell level. Objective We aimed to generate a single-cell omics-based…
View article: CRISPR/Cas9-mediated gene disruption determines the roles of MITF and CITED2 in human mast cell differentiation
CRISPR/Cas9-mediated gene disruption determines the roles of MITF and CITED2 in human mast cell differentiation Open
A CRISPR/Cas9-based framework allows the identification of genes involved in human mast cell differentiation
View article: No indication of aberrant neutrophil extracellular trap release in indolent or advanced systemic mastocytosis
No indication of aberrant neutrophil extracellular trap release in indolent or advanced systemic mastocytosis Open
In disease states with chronic inflammation, there is a crosstalk between mast cells and neutrophil granulocytes in the inflamed microenvironment, which may be potentiated by tryptase. In systemic mastocytosis (SM), mast cells are constitu…
View article: Analysis of human lung mast cells by single cell RNA sequencing
Analysis of human lung mast cells by single cell RNA sequencing Open
Mast cells are tissue-resident cells playing major roles in homeostasis and disease conditions. Lung mast cells are particularly important in airway inflammatory diseases such as asthma. Human mast cells are classically divided into the su…
View article: A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells
A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells Open
Innate lymphoid cells (ILCs) play important roles in tissue homeostasis and host defense, but the proliferative properties and migratory behavior of especially human ILCs remain poorly understood. Here we mapped at single-cell resolution t…
View article: Single-cell transcriptomics reveals the identity and regulators of human mast cell progenitors
Single-cell transcriptomics reveals the identity and regulators of human mast cell progenitors Open
Mast cell accumulation is a hallmark of a number of diseases, including allergic asthma and systemic mastocytosis. Immunoglobulin E–mediated crosslinking of the FcεRI receptors causes mast cell activation and contributes to disease pathoge…
View article: The Proportion of FceRI+ Blood Monocytes Increases With the Degree of IgE-Mediated Sensitization in Asthma
The Proportion of FceRI+ Blood Monocytes Increases With the Degree of IgE-Mediated Sensitization in Asthma Open
Study participantsThe study included 67 individuals between 15-41 years old in a 3-5 year follow-up of the MIDAS cohort (Minimally Invasive Diagnostic Procedures in Allergy, Asthma, or Food hypersensitivity), MIDASII.Data from MIDASII have…
View article: Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity Open
Background Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensiv…
View article: Single-cell transcriptomics reveals the identity and regulators of human mast cell progenitors
Single-cell transcriptomics reveals the identity and regulators of human mast cell progenitors Open
Mast cell accumulation is a hallmark of a number of diseases including allergic asthma and systemic mastocytosis. IgE-mediated crosslinking of the FcεRI receptors causes mast cell activation and contributes to disease pathogenesis. The mas…
View article: The ingenious mast cell: Contemporary insights into mast cell behavior and function
The ingenious mast cell: Contemporary insights into mast cell behavior and function Open
Mast cells are (in)famous for their role in allergic diseases, but the physiological and pathophysiological roles of this ingenious cell are still not fully understood. Mast cells are important for homeostasis and surveillance of the human…
View article: Immunoprofiling reveals novel mast cell receptors and the continuous nature of human lung mast cell heterogeneity
Immunoprofiling reveals novel mast cell receptors and the continuous nature of human lung mast cell heterogeneity Open
Background: Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensi…
View article: The Ingenious Mast Cell: Contemporary insights into mast cell behavior and function
The Ingenious Mast Cell: Contemporary insights into mast cell behavior and function Open
Mast cells are (in)famous for their role in allergic diseases, but the physiological and pathophysiological roles of this ingenious cell are still not fully understood. Mast cells are important for homeostasis and surveillance of the human…
View article: Single‐cell molecular profiling provides a high‐resolution map of basophil and mast cell development
Single‐cell molecular profiling provides a high‐resolution map of basophil and mast cell development Open
Background Basophils and mast cells contribute to the development of allergic reactions. Whereas these mature effector cells are extensively studied, the differentiation trajectories from hematopoietic progenitors to basophils and mast cel…
View article: Localization-Specific Expression of CCR1 and CCR5 by Mast Cell Progenitors
Localization-Specific Expression of CCR1 and CCR5 by Mast Cell Progenitors Open
Mast cells are powerful immune cells found predominately in barrier tissues. They play an important role in immune surveillance and act as effector cells in allergic reactions. Mast cells develop from mast cell progenitors (MCp), which mig…
View article: Single-cell molecular profiling provides a high-resolution map of basophil and mast cell differentiation
Single-cell molecular profiling provides a high-resolution map of basophil and mast cell differentiation Open
Differentiation of hematopoietic stem and progenitor cells ensure a continuous supply of mature blood cells. Recent models of differentiation are represented as a landscape, in which individual progenitors traverse a continuum of multipote…
View article: CD203c distinguishes the erythroid and mast cell‐basophil differentiation trajectories among human FcεRI<sup>+</sup> bone marrow progenitors
CD203c distinguishes the erythroid and mast cell‐basophil differentiation trajectories among human FcεRI<sup>+</sup> bone marrow progenitors Open
IgE molecules that bind their specific antigen crosslink FcεRI receptors present on mast cells and basophils. Downstream signaling results in cell activation and subsequent release of diverse compounds that exhibit potential to trigger all…
View article: Single-cell analysis reveals the KIT D816V mutation in haematopoietic stem and progenitor cells in systemic mastocytosis
Single-cell analysis reveals the KIT D816V mutation in haematopoietic stem and progenitor cells in systemic mastocytosis Open
The KIT D816V mutation arises in early haematopoietic stem and progenitor cells and the mutation frequency is approaching 100% in mature mast cells, which express the aberrant marker CD45RA.
View article: Circulating mast cell progenitors correlate with reduced lung function in allergic asthma
Circulating mast cell progenitors correlate with reduced lung function in allergic asthma Open
Summary Background Studies using mouse models have revealed that mast cell progenitors are recruited from the blood circulation to the lung during acute allergic airway inflammation. The discovery of a corresponding human mast cell progeni…
View article: PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells
PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells Open
View article: An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells
An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells Open
Background: Mast cells are tissue-resident inflammatory cells defined by their high granularity and surface expression of the high-affinity IgE receptor, FcεRI, and CD117/KIT, the receptor for stem cell factor (SCF). There is a cons…
View article: Deciphering the differentiation trajectory from hematopoietic stem cells to mast cells
Deciphering the differentiation trajectory from hematopoietic stem cells to mast cells Open
Hematopoietic stem cells differentiate into all types of blood cells, including peripheral tissue-resident mast cells. The early mast cell differentiation takes place in the bone marrow, after which the progenitor cells enter the circulati…
View article: Single-cell analysis reveals the <i>KIT</i> D816V mutation in hematopoietic stem and progenitor cells in systemic mastocytosis: Supplementary data
Single-cell analysis reveals the <i>KIT</i> D816V mutation in hematopoietic stem and progenitor cells in systemic mastocytosis: Supplementary data Open
Background: Systemic mastocytosis (SM) is a hematological disease characterized by organ infiltration by neoplastic mast cells. Almost all SM patients have a mutation in the gene encoding the tyrosine kinase receptor KIT causing a D816V su…
View article: A single-cell hematopoietic landscape resolves 8 lineage trajectories and defects in Kit mutant mice
A single-cell hematopoietic landscape resolves 8 lineage trajectories and defects in Kit mutant mice Open
Key Points Single-cell transcriptional landscape of 44 802 hematopoietic stem/progenitor cells defines entry points to 8 different blood lineages. Comparison with 13 815 c-Kit mutant cells identifies pleiotropic changes in cell type abunda…
View article: Graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells
Graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells Open
Single-cell RNA-seq quantifies biological heterogeneity across both discrete cell types and continuous cell transitions. Partition-based graph abstraction (PAGA) provides an interpretable graph-like map of the arising data manifold, based …
View article: KIT signaling is dispensable for human mast cell progenitor development
KIT signaling is dispensable for human mast cell progenitor development Open
Key Points SCF and KIT signaling are dispensable for the survival, proliferation, and maturation of human mast cell progenitors.
View article: Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia Open
View article: Influenza Infection in Mice Induces Accumulation of Lung Mast Cells through the Recruitment and Maturation of Mast Cell Progenitors
Influenza Infection in Mice Induces Accumulation of Lung Mast Cells through the Recruitment and Maturation of Mast Cell Progenitors Open
Mast cells (MCs) are powerful immune cells that mature in the peripheral tissues from bone marrow (BM)-derived mast cell progenitors (MCp). Accumulation of MCs in lung compartments where they are normally absent is thought to enhance sympt…
View article: IgE-mediated enhancement of CD4+ T cell responses requires antigen presentation by CD8α− conventional dendritic cells
IgE-mediated enhancement of CD4+ T cell responses requires antigen presentation by CD8α− conventional dendritic cells Open
IgE, forming an immune complex with small proteins, can enhance the specific antibody and CD4 + T cell responses in vivo . The effects require the presence of CD23 (Fcε-receptor II) + B cells, which capture IgE-complexed antigens (Ag) in t…
View article: Lin− CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitors
Lin− CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitors Open
Key Points Human Lin− CD34hi CD117int/hi FcεRI+ cells in blood constitute mast cell progenitors. Asthmatics with reduced lung function have an increased frequency of circulating mast cell progenitors.
View article: Distinguishing Mast Cell Progenitors from Mature Mast Cells in Mice
Distinguishing Mast Cell Progenitors from Mature Mast Cells in Mice Open
Mast cells originate from the bone marrow and develop into c-kit(+) FcɛRI(+) cells. Both mast cell progenitors (MCp) and mature mast cells express these cell surface markers, and ways validated to distinguish between the two maturation for…