Joan E. McEwen
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<i>Histoplasma capsulatum</i>and<i>Caenorhabditis elegans</i>: a simple nematode model for an innate immune response to fungal infection Open
Histoplasma capsulatum is a primary fungal pathogen of mammals responsible for histoplasmosis. During pathogenesis H. capsulatum yeast proliferate in phagosomes of macrophages. This extensive host/pathogen interaction involves a complex ca…
Increasing NADH oxidation reduces overflow metabolism in <i>Saccharomyces cerevisiae</i> Open
Respiratory metabolism plays an important role in energy production in the form of ATP in all aerobically growing cells. However, a limitation in respiratory capacity results in overflow metabolism, leading to the formation of byproducts, …
Mechanisms of Resistance to Oxidative and Nitrosative Stress: Implications for Fungal Survival in Mammalian Hosts Open
The ability of a fungal pathogen to cause disease requires the ability to survive in the host. Survival in the host is dependent on evasion of the host's immune system, including the microbial killing mechanisms of phagocytes. The innate i…
The Translational Regulation of Lipoprotein Lipase by Epinephrine Involves an RNA Binding Complex Including the Catalytic Subunit of Protein Kinase A Open
The balance of lipid flux in adipocytes is controlled by the opposing actions of lipolysis and lipogenesis, which are controlled primarily by hormone-sensitive lipase and lipoprotein lipase (LPL), respectively. Catecholamines stimulate adi…
Expression of the divergent transcription unit containing the yeast PET122 and OXA1 genes Open
The nuclear PET122 gene of S. cerevisiae encodes a mitochondrial‐localized protein that activates initiation of translation of the mitochondrial mRNA from the COX3 gene, which encodes subunit III of cytochrome c oxidase. The PET122 locus c…
The <i>Saccharomyces cerevisiae</i> Pet309 protein is embedded in the mitochondrial inner membrane Open
The nuclear PET309 gene of Saccharomyces cerevisiae is necessary for expression of the mitochondrial COX1 gene, which encodes subunit I of cytochrome c oxidase. In a pet309 null mutant, there is a defect both in accumulation of COX1 pre‐RN…
Genetic evidence that different functional domains of the PET54 gene product facilitate expression of the mitochondrial genes COX1 and COX3 in Saccharomyces cerevisiae. Open
Expression of the yeast mitochondrial genes COX1 and COX3, which encode subunits I and III of cytochrome oxidase, respectively, is controlled by a common nuclear-encoded trans-acting factor. This protein, encoded by the PET54 gene, control…
Divergent overlapping transcripts at the PET122 locus in Saccharomyces cerevisiae. Open
PET122 is one of three nuclear genes specifically required for translation of the mitochondrial mRNA for cytochrome c oxidase subunit III in Saccharomyces cerevisiae. The nucleotide sequence of 2,862 base pairs (bp) of yeast genomic DNA en…
Nucleotide sequence of the gene encoding cytochrome c oxidase subunit VII from<i>Saccharomyces cerevisiae</i> Open
Journal Article Nucleotide sequence of the gene encoding cytochrome c oxidase subunit VII from Saccharomyces cerevisiae Kathleen M. Calder, Kathleen M. Calder Department of Microbiology, University of California, 405 Hilgard Avenue, Los An…
Molecular cloning and nucleotide sequence of the nuclear PET122 gene required for expression of the mitochondrial COX3 gene in S.cerevisiae Open
The nuclear PET122 gene from S. cerevisiae is necessary for translation of a single mitochondrial mRNA that encodes subunit III of cytochrome c oxidase. We report here the cloning and nucleotide sequence of PET122, and properties of the pr…
Identification of a third nuclear protein-coding gene required specifically for posttranscriptional expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae Open
A third nuclear protein-coding gene termed PET122 has been shown to be required for a post-transcriptional step in expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae. pet122 mutants fail to produce cytochrome c oxidase s…
Nuclear functions required for cytochrome c oxidase biogenesis in Saccharomyces cerevisiae. Characterization of mutants in 34 complementation groups. Open
To identify nuclear functions required for cytochrome c oxidase biogenesis in yeast, recessive nuclear mutants that are deficient in cytochrome c oxidase were characterized. In complementation studies, 55 independently isolated mutants wer…
Rapid method for isolation and screening of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae Open
We describe here a new method for the specific isolation of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae. One unique feature of the method is the use of tetramethyl-p-phenylenediamine as a cytochrome c oxidase activit…
Mitochondrial gene expression in saccharomyces cerevisiae. II. Fidelity of translation in isolated mitochondria from wild type and respiratory-deficient mutant cells. Open
The fidelity of mitochondrial translation has been examined in isolated yeast mitochondria incubated in an optimized protein-synthesizing medium (McKee, E. E., and Poyton, R. O., (1984) J. Biol. Chem. 259, 9320-9331). These studies have re…
Nuclear genes for mitochondrial proteins. Identification and isolation of a structural gene for subunit V of yeast cytochrome c oxidase. Open
The gene for yeast cytochrome c oxidase subunit V, COX5, has been isolated from a Saccharomyces cerevisiae DNA library by complementation of a cytochrome c oxidase subunit V mutant, JM28. One complementing plasmid, YEp13-511, with a DNA in…
Synthesis of outer membrane proteins in cpxA cpxB mutants of Escherichia coli K-12 Open
Two major proteins, the murein lipoprotein and the OmpF matrix porin, are deficient in the outer membrane of cpxA cpxB mutants of Escherichia coli K-12. We present evidence that the cpx mutations prevent or retard the translocation of thes…
Mutations in genes cpxA and cpxB alter the protein composition of Escherichia coli inner and outer membranes Open
Mutations in chromosomal genes cpxA and cpxB altered the protein composition of the inner and outer bacterial membranes. Electrophoretic analyses of membrane proteins from isogenic strains differing only at their cpx loci and of spontaneou…
Mutations in genes cpxA and cpxB of Escherichia coli K-12 cause a defect in acetohydroxyacid synthase I function in vivo Open
Mutations in Escherichia coli genes cpxA and cpxB together cause a temperature-sensitive defect in isoleucine and valine syntheses that is related specifically to acetohydroxyacid synthase I. This enzyme catalyzes the first pair of homolog…
Mutations in genes cpxA and cpxB of Escherichia coli K-12 cause a defect in isoleucine and valine syntheses Open
Mutations in two chromosomal genes of Escherichia coli, cpxA and cpxB, produced a temperature-sensitive growth defect that was remedied specifically by the addition of isoleucine and valine to the minimal medium. This auxotrophy was manife…
Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB Open
Two temperature-sensitive, chromosomal mutants of Escherichia coli were selected for their inability to express deoxyribonucleic acid donor activity and other activities associated with the conjugative plasmid F. These mutants were also au…
Selection of Escherichia coli K-12 chromosomal mutants that prevent expression of F-plasmid functions Open
Chromosomal mutants of Escherichia coli deficient in the expression of F-plasmid functions were selected by mutagenizing F- cells, introducing an F' plasmid into the mutagenized cells by conjugation, and identifying transconjugants resista…
Chromosomal mutations of Escherichia coli that alter expression of conjugative plasmid functions. Open
We have identified two chromosomal genes of Escherichia coli K12 that are required for the expression of conjugative plasmid functions in the presence of normal plasmid DNA. Hfr cells with mutations in both of these genes are resistant to …