Joann L. Prior
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View article: Single-stranded DNA oligonucleotides containing CpG motifs are non-stimulatory in vitro but offer protection in vivo against Burkholderia pseudomallei
Single-stranded DNA oligonucleotides containing CpG motifs are non-stimulatory in vitro but offer protection in vivo against Burkholderia pseudomallei Open
Therapies that modulate and appropriately direct the immune response are promising candidates for the treatment of infectious diseases. One such candidate therapeutic is DZ13, a short, synthetic, single-stranded DNA molecule. This molecule…
View article: The Arabinose 5-Phosphate Isomerase KdsD Is Required for Virulence in Burkholderia pseudomallei
The Arabinose 5-Phosphate Isomerase KdsD Is Required for Virulence in Burkholderia pseudomallei Open
The intrinsic resistance of B. pseudomallei to many antibiotics complicates treatment. This opportunistic pathogen possesses a wide range of virulence factors, resulting in severe and potentially fatal disease.
View article: Spinning sugars in antigen biosynthesis: characterization of the Coxiella burnetii and Streptomyces griseus TDP-sugar epimerases
Spinning sugars in antigen biosynthesis: characterization of the Coxiella burnetii and Streptomyces griseus TDP-sugar epimerases Open
View article: Antibiotic-Loaded Polymersomes for Clearance of Intracellular <i>Burkholderia thailandensis</i>
Antibiotic-Loaded Polymersomes for Clearance of Intracellular <i>Burkholderia thailandensis</i> Open
Melioidosis caused by the facultative intracellular pathogen Burkholderia pseudomallei is difficult to treat due to poor intracellular bioavailability of antibiotics and antibiotic resistance. In the absence of novel compounds, poly…
View article: Spinning sugars in antigen biosynthesis: a direct study of the <i>Coxiella burnetii</i> and <i>Streptomyces griseus</i> TDP-sugar epimerases
Spinning sugars in antigen biosynthesis: a direct study of the <i>Coxiella burnetii</i> and <i>Streptomyces griseus</i> TDP-sugar epimerases Open
The sugars streptose and dihydrohydroxystreptose (DHHS) are unique to the bacteria Streptomyces griseus and Coxiella burnetii respectively. Streptose forms the central moiety of the antibiotic streptomycin, whilst DHHS is found in the O-an…
View article: Monoclonal Antibodies Opsonize <i>Burkholderia</i> spp. and Reduce Intracellular Actin Tail Formation in a Macrophage Infection Assay
Monoclonal Antibodies Opsonize <i>Burkholderia</i> spp. and Reduce Intracellular Actin Tail Formation in a Macrophage Infection Assay Open
Melioidosis is difficult to treat successfully due to the causal bacterium being resistant to many classes of antibiotics, therefore limiting available therapeutic options. New and improved therapies are urgently required to treat this dis…
View article: In Vitro Priming of Human T Cells by Dendritic Cells Provides a Screening Tool for Candidate Vaccines for Burkholderia pseudomallei
In Vitro Priming of Human T Cells by Dendritic Cells Provides a Screening Tool for Candidate Vaccines for Burkholderia pseudomallei Open
Murine dendritic cells, when pulsed with heat-killed Burkholderia pseudomallei and used to immunise naïve mice, have previously been shown to induce protective immunity in vivo. We have now demonstrated the in vitro priming of naïve human …
View article: Role for both myeloid and plasmacytoid DC in driving primary T helper cell response to<i>Burkholderia pseudomallei</i>in healthy individuals
Role for both myeloid and plasmacytoid DC in driving primary T helper cell response to<i>Burkholderia pseudomallei</i>in healthy individuals Open
Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, an infectious disease endemic to south-east Asia. As B. pseudomallei is antibiotic-resistant, the need for cell-based vaccines and therapies is crucial to mana…
View article: Assessments of hepatitis B virus-like particles and Crm197 as carrier proteins in melioidosis glycoconjugate vaccines
Assessments of hepatitis B virus-like particles and Crm197 as carrier proteins in melioidosis glycoconjugate vaccines Open
The Tier 1 select agent Burkholderia pseudomallei is the causative agent of melioidosis, a global pathogen and a major cause of pneumonia and sepsis for which no licensed vaccines currently exist. Previous work has shown the potential for …
View article: UK vaccines network: Mapping priority pathogens of epidemic potential and vaccine pipeline developments
UK vaccines network: Mapping priority pathogens of epidemic potential and vaccine pipeline developments Open
During the 2013-2016 Ebola outbreak in West Africa an expert panel was established on the instructions of the UK Prime Minister to identify priority pathogens for outbreak diseases that had the potential to cause future epidemics. A total …
View article: Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever
Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever Open
Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in …
View article: Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
A weakness of studies using B. thailandensis as a surrogate for B. pseudomallei is that the strains used replicate at a slower rate in infected cells. We show that the pattern of growth of B. thailandensis strain E555 in macrophages closel…
View article: Genome Resequencing of Laboratory Stocks of Burkholderia pseudomallei K96243
Genome Resequencing of Laboratory Stocks of Burkholderia pseudomallei K96243 Open
We have resequenced the genomes of four Burkholderia pseudomallei K96243 laboratory cultures and compared them to the reported genome sequence that was published in 2004. Compared with the reference genome, these laboratory cultures harbor…
View article: Additional file 9: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 9: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S7. Lists of genes in each group shown in Table 2. (XLSX 321 kb)
View article: Additional file 5: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 5: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S3. Transcripts detected in vitro and in vivo. (XLSX 2144 kb)
View article: Additional file 4: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 4: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S2. Gene expression results and details of the genes subjected to RT-qPCR analysis. (XLSX 15 kb)
View article: Additional file 8: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 8: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S6. Known virulence genes. (XLSX 208 kb)
View article: Additional file 6: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 6: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S4. Proteins detected in vitro and in vivo. (XLSX 1095 kb)
View article: Additional file 3: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 3: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S1. Details of B. thailandensis strain E555 genes and proteins. (XLSX 6051 kb)
View article: Additional file 7: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages
Additional file 7: of Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages Open
Table S5. Significantly regulated genes, capsule expression and all expression data. (XLSX 2279 kb)
View article: An O-Antigen Glycoconjugate Vaccine Produced Using Protein Glycan Coupling Technology Is Protective in an Inhalational Rat Model of Tularemia
An O-Antigen Glycoconjugate Vaccine Produced Using Protein Glycan Coupling Technology Is Protective in an Inhalational Rat Model of Tularemia Open
There is a requirement for an efficacious vaccine to protect people against infection from Francisella tularensis , the etiological agent of tularemia. The lipopolysaccharide (LPS) of F. tularensis is suboptimally protective against a pare…
View article: Zoonoses under our noses
Zoonoses under our noses Open
View article: VALIDATE: Exploiting the synergy between complex intracellular pathogens to expedite vaccine research and development for tuberculosis, leishmaniasis, melioidosis and leprosy
VALIDATE: Exploiting the synergy between complex intracellular pathogens to expedite vaccine research and development for tuberculosis, leishmaniasis, melioidosis and leprosy Open
For several complex intracellular pathogens, we have an urgent need for effective vaccines and yet there are common barriers to vaccine development. These diseases, including tuberculosis, leishmaniasis, leprosy and melioidosis, cause a hu…
View article: Structural characterisation of the capsular polysaccharide expressed by Burkholderia thailandensis strain E555:: wbiI (pKnock-KmR) and assessment of the significance of the 2-O-acetyl group in immune protection
Structural characterisation of the capsular polysaccharide expressed by Burkholderia thailandensis strain E555:: wbiI (pKnock-KmR) and assessment of the significance of the 2-O-acetyl group in immune protection Open
View article: Mapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection
Mapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection Open
View article: Protection against Experimental Melioidosis with a Synthetic <i>manno</i>-Heptopyranose Hexasaccharide Glycoconjugate
Protection against Experimental Melioidosis with a Synthetic <i>manno</i>-Heptopyranose Hexasaccharide Glycoconjugate Open
Melioidosis is an emerging infectious disease caused by Burkholderia pseudomallei and is associated with high morbidity and mortality rates in endemic areas. Antibiotic treatment is protracted and not always successful; even with appropria…
View article: Immunisation with proteins expressed during chronic murine melioidosis provides enhanced protection against disease
Immunisation with proteins expressed during chronic murine melioidosis provides enhanced protection against disease Open
View article: Differential Toll-Like Receptor-Signalling of Burkholderia pseudomallei Lipopolysaccharide in Murine and Human Models
Differential Toll-Like Receptor-Signalling of Burkholderia pseudomallei Lipopolysaccharide in Murine and Human Models Open
The Gram-negative bacterium Burkholderia pseudomallei causes melioidosis and is a CDC category B bioterrorism agent. Toll-like receptor (TLR)-2 impairs host defense during pulmonary B.pseudomallei infection while TLR4 only has limited impa…
View article: Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery
Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery Open
View article: Systematic Mutagenesis of Genes Encoding Predicted Autotransported Proteins of Burkholderia pseudomallei Identifies Factors Mediating Virulence in Mice, Net Intracellular Replication and a Novel Protein Conferring Serum Resistance
Systematic Mutagenesis of Genes Encoding Predicted Autotransported Proteins of Burkholderia pseudomallei Identifies Factors Mediating Virulence in Mice, Net Intracellular Replication and a Novel Protein Conferring Serum Resistance Open
Burkholderia pseudomallei is the causative agent of the severe tropical disease melioidosis, which commonly presents as sepsis. The B. pseudomallei K96243 genome encodes eleven predicted autotransporters, a diverse family of secreted and o…