Joanna Simpson
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View article: Pharmacological inhibition of hydroxysteroid 11‐beta dehydrogenase type 1 (11‐βHSD1) after myocardial infarction preserves cardiac function in a translational mini‐pig model
Pharmacological inhibition of hydroxysteroid 11‐beta dehydrogenase type 1 (11‐βHSD1) after myocardial infarction preserves cardiac function in a translational mini‐pig model Open
Background and Purpose Plasma glucocorticoids increase acutely after MI, thereafter tissue levels are amplified selectively within cells expressing 11‐ ß hydroxysteroid dehydrogenase type 1 (11‐ßHSD1) that regenerates active glucocorticoid…
Patient-Centred Preoperative Assessment Open
Patient-centred preoperative assessment describes an approach to preoperative assessment based on early screening and triage, optimisation of comorbidities and lifestyle, shared decision-making and multidisciplinary working. This approach …
A survey of the workload generated by older surgical patients referred to on-call medical registrars—SNAP-3 Open
The findings highlight the significant workload and training gaps faced by medical registrars in managing older surgical patients. Bridging the gap between national recommendations and local services may reduce demands on on-call registrar…
Temporal dichotomy of neutrophil function in acute liver injury and repair Open
Novel therapies are required for APAP-ALI to improve patient outcomes. Neutrophil products and functions are potential targets for future therapies, but current literature controversy and a lack of time-dependent studies hinder progression…
Sensitive targeted analysis of salivary steroids by liquid chromatography mass spectrometry for studies of infertility v1 Open
Progesterone plays a key role in implantation and early pregnancy (Mesen et al, 2015) and studies have shown that low blood progesterone in the luteal phase result in lower pregnancy rates and an increased pregnancy loss rate following fre…
View article: Long COVID and menstruation: bidirectional associations and potential mechanisms.
Long COVID and menstruation: bidirectional associations and potential mechanisms. Open
Women have reported both menstrual disruption following SARS-CoV-2 infection and that long COVID symptoms change across the menstrual cycle. Yet there is a lack of female-specific research on COVID and menstruation. We aimed to determine i…
View article: Pharmacological inhibition of 11ßhydroxysteroid dehydrogenase type 1 after myocardial infarction targets extracellular matrix processing and preserves cardiac function in a translational mini-pig model
Pharmacological inhibition of 11ßhydroxysteroid dehydrogenase type 1 after myocardial infarction targets extracellular matrix processing and preserves cardiac function in a translational mini-pig model Open
Background and Purpose Plasma glucocorticoids (GCs) increase acutely after myocardial infarction (MI), thereafter tissue levels are amplified selectively within cells expressing 11ßHydroxysteroid Dehydrogenase type 1 (11ßHSD1) that regener…
View article: Proof of concept for a superior therapeutic index of corticosterone compared with hydrocortisone in patients with congenital adrenal hyperplasia
Proof of concept for a superior therapeutic index of corticosterone compared with hydrocortisone in patients with congenital adrenal hyperplasia Open
Objective Outcomes are poor for patients with congenital adrenal hyperplasia (CAH), in part due to the supraphysiological glucocorticoid doses required to control adrenal androgen excess. Hydrocortisone (ie, cortisol) is the recommended gl…
View article: Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis
Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis Open
Summary Endometriosis is a common and debilitating neuro-inflammatory disorder that is associated with chronic pain. Definitive diagnosis is based on the presence of endometrial-like tissue (lesions) in sites outside the uterus. Kynurenine…
View article: Repurposing a Fully Reducing Polyketide Synthase toward 2-Methyl Guerbet-like Lipids
Repurposing a Fully Reducing Polyketide Synthase toward 2-Methyl Guerbet-like Lipids Open
In nature, thousands of diverse and bioactive polyketides are assembled by a family of multifunctional, "assembly line" enzyme complexes called polyketide synthases (PKS). Since the late 20th century, there have been several attempts to de…
View article: Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgia, and rescues mice from a negative behavioural phenotype in experimental endometriosis
Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgia, and rescues mice from a negative behavioural phenotype in experimental endometriosis Open
Summary Endometriosis is a common and debilitating neuro-inflammatory disorder that is associated with chronic pain. Definitive diagnosis is based on the presence of endometrial-like tissue (lesions) in sites outside the uterus. Kynurenine…
View article: Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis
Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis Open
Summary Endometriosis is a common and debilitating neuro-inflammatory disorder that is associated with chronic pain. Definitive diagnosis is based on the presence of endometrial-like tissue (lesions) in sites outside the uterus. Kynurenine…
View article: Repurposing a Fully-Reducing Polyketide Synthase for 2-Methyl Guerbet-like Lipid Production
Repurposing a Fully-Reducing Polyketide Synthase for 2-Methyl Guerbet-like Lipid Production Open
In Nature, thousands of diverse and bioactive polyketides are assembled by a family of multifunctional, “assembly line” enzyme complexes called Polyketide Synthases (PKS). Since the late 20th century, there have been several attempts to de…
View article: ATP-binding cassette family C member 1 constrains metabolic responses to high-fat diet in male mice
ATP-binding cassette family C member 1 constrains metabolic responses to high-fat diet in male mice Open
Glucocorticoids modulate glucose homeostasis, acting on metabolically active tissues such as liver, skeletal muscle, and adipose tissue. Intracellular regulation of glucocorticoid action in adipose tissue impacts metabolic responses to obe…
Carbonyl Reductase 1: a novel regulator of blood pressure in Down Syndrome Open
Background Approximately one in every 800 children is born with the severe aneuploid condition of Down Syndrome (DS), a trisomy of chromosome 21. Low blood pressure (hypotension) is a common condition associated with DS and can have a sign…
View article: Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis
Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis Open
SUMMARY Endometriosis is a common and debilitating neuro-inflammatory disorder that is associated with chronic pain. Definitive diagnosis is based on the presence of endometrial-like tissue (lesions) in sites outside the uterus. Kynurenine…
View article: Plasma steroid concentrations reflect acute disease severity and normalise during recovery in people hospitalised with COVID‐19
Plasma steroid concentrations reflect acute disease severity and normalise during recovery in people hospitalised with COVID‐19 Open
Objective Endocrine systems are disrupted in acute illness, and symptoms reported following coronavirus disease 2019 (COVID‐19) are similar to those found with clinical hormone deficiencies. We hypothesised that people with severe acute CO…
View article: Systematic review and longitudinal analysis of implementing Artificial Intelligence to predict clinical deterioration in adult hospitals: what is known and what remains uncertain
Systematic review and longitudinal analysis of implementing Artificial Intelligence to predict clinical deterioration in adult hospitals: what is known and what remains uncertain Open
Objective To identify factors influencing implementation of machine learning algorithms (MLAs) that predict clinical deterioration in hospitalized adult patients and relate these to a validated implementation framework. Materials and metho…
Using TraceFinder and Excel software to evaluate and report multi-analyte targeted LC-MS data acquired on an ThermoScientific Exploris 240 Orbitrap v1 Open
In our lab we focus on targeted analysis and have found that acquiring LC-HRMS data using Xcalibur software and assessing the data using TraceFinder software is the most robust and flexible approach. This protocol describes our approach to…
Targeted analysis of Short Chain Fatty Acids (SCFAs) in human serum using derivatization and LC-HRMS analysis v1 Open
This protocol describes the sample preparation and analysis of short chain fatty acids in human serum samples. Short chain fatty acids (SCFAs) - acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and …
Targeted analysis of 5-Fluorouracil (5-FU) and Fluoroacetate (FAC) in human plasma by automated PPT+ extraction and LC-HRMS analysis v1 Open
This protocol describes the extraction and targeted high-resolution mass spectrometry analysis of two compounds - the anti-cancer drug 5-fluorouracil (5FU) and its catabolite fluoroacetate (FAC), in human plasma samples. A targeted LC-MS m…
Analysis of AT7519 as a pro-resolution compound in an acetaminophen-induced mouse model of acute inflammation by UPLC-MS/MS Open
Background Uncontrolled inflammation contributes to the progression of organ damage in acute conditions, such as acetaminophen-induced acute liver injury (APAP-ALI) and there are limited treatments for this condition. AT7519, a cyclic-depe…