Joaquím Roca
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View article: Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction
Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction Open
Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo development. In some mammals, like pigs, ejaculates are emitted in three separate fractions: pre-s…
View article: Nucleosomal DNA has topological memory
Nucleosomal DNA has topological memory Open
One fundamental yet elusive aspect of chromosome architecture is the topolome, which refers to how chromatin elements shape the path of DNA. Nucleosomes stabilise negative DNA supercoils, with most nucleosomes typically restraining a DNA l…
View article: Knowledge, attitudes, and practices about COVID-19 pandemic: a bibliometric analysis
Knowledge, attitudes, and practices about COVID-19 pandemic: a bibliometric analysis Open
Background In order to avoid high rates of COVID-19 infection, one of the main tasks that must be performed is to improve the knowledge, attitudes, and practices (KAP) about the virus. In this sense, Health Education is an essential tool f…
View article: Mating modifies the expression of crucial oxidative-reductive transcripts in the pig oviductal sperm reservoir: is the female ensuring sperm survival?
Mating modifies the expression of crucial oxidative-reductive transcripts in the pig oviductal sperm reservoir: is the female ensuring sperm survival? Open
Background Mating induces large changes in the female genital tract, warranting female homeostasis and immune preparation for pregnancy, including the preservation of crucial oxidative status among its pathways. Being highly susceptible to…
View article: Nucleosomal DNA has topological memory
Nucleosomal DNA has topological memory Open
SUMMARY One fundamental yet elusive aspect of the chromosome architecture is the constrained topolome, which refers to how chromatin elements restrain DNA topology. Nucleosomes stabilise negative DNA supercoils, with most nucleosomes typic…
View article: Structural analysis of the <i>Candida albicans</i> mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism
Structural analysis of the <i>Candida albicans</i> mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism Open
The compaction of mitochondrial DNA (mtDNA) is regulated by architectural HMG-box proteins whose limited cross-species similarity suggests diverse underlying mechanisms. Viability of Candida albicans, a human antibiotic-resistant mucosal p…
View article: Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients Open
Here, we examined the dynamics of the gut and respiratory microbiomes in severe COVID-19 patients in need of mechanical ventilation in the intensive care unit (ICU). We recruited 85 critically ill patients (53 with COVID-19 and 32 without …
View article: Dysbiosis: an indicator of COVID-19 severity in critically ill patients
Dysbiosis: an indicator of COVID-19 severity in critically ill patients Open
Background : Research into the association between the microbiome and SARS-CoV-2 infection and acute respiratory distress syndrome remains unclear. Here we examined the dynamics of the gut and respiratory microbiomes in severe COVID-19 cas…
View article: Condensin pinches a short negatively supercoiled DNA loop during each round of ATP usage
Condensin pinches a short negatively supercoiled DNA loop during each round of ATP usage Open
SUMMARY Condensin extrudes DNA loops using an ATP-dependent mechanism that remains to be elucidated. Here, we show how condensin activity alters the topology of the interacting DNA. High condensin concentrations restrain DNA positive super…
View article: Keeping intracellular DNA untangled: A new role for condensin?
Keeping intracellular DNA untangled: A new role for condensin? Open
The DNA‐passage activity of topoisomerase II accidentally produces DNA knots and interlinks within and between chromatin fibers. Fortunately, these unwanted DNA entanglements are actively removed by some mechanism. Here we present an outli…
View article: Dysbiosis and relapse-related microbiome in inflammatory bowel disease: A shotgun metagenomic approach
Dysbiosis and relapse-related microbiome in inflammatory bowel disease: A shotgun metagenomic approach Open
Crohn's disease (CD) and ulcerative colitis (UC), the two main forms of inflammatory bowel disease (IBD), affect several million people worldwide. CD and UC are characterized by periods of clinical remission and relapse. Although IBD patie…
View article: Transcriptional supercoiling boosts topoisomerase II-mediated knotting of intracellular DNA
Transcriptional supercoiling boosts topoisomerase II-mediated knotting of intracellular DNA Open
Recent studies have revealed that the DNA cross-inversion mechanism of topoisomerase II (topo II) not only removes DNA supercoils and DNA replication intertwines, but also produces small amounts of DNA knots within the clusters of nucleoso…
View article: Quantitative disclosure of DNA knot chirality by high-resolution 2D-gel electrophoresis
Quantitative disclosure of DNA knot chirality by high-resolution 2D-gel electrophoresis Open
The characterization of knots formed in duplex DNA has proved useful to infer biophysical properties and the spatial trajectory of DNA, both in free solution and across its macromolecular interactions. Since knotting, like supercoiling, ma…
View article: Structure and Chromosomal Organization of Yeast Genes Regulated by Topoisomerase II
Structure and Chromosomal Organization of Yeast Genes Regulated by Topoisomerase II Open
Cellular DNA topoisomerases (topo I and topo II) are highly conserved enzymes that regulate the topology of DNA during normal genome transactions, such as DNA transcription and replication. In budding yeast, topo I is dispensable whereas t…
View article: DNA knots occur in intracellular chromatin
DNA knots occur in intracellular chromatin Open
In vivo DNA molecules are narrowly folded within chromatin fibers and self-interacting chromatin domains. Therefore, intra-molecular DNA entanglements (knots) might occur via DNA strand passage activity of topoisomerase II. Here, we assess…
View article: Genome urbanization: clusters of topologically co-regulated genes delineate functional compartments in the genome of Saccharomyces cerevisiae
Genome urbanization: clusters of topologically co-regulated genes delineate functional compartments in the genome of Saccharomyces cerevisiae Open
The eukaryotic genome evolves under the dual constraint of maintaining coordinated gene transcription and performing effective DNA replication and cell division, the coupling of which brings about inevitable DNA topological tension. DNA su…
View article: Chromosomal DNA Topology: Topoisomerases Handle Extremely Supercoiled, Catenated and Knotted Chromatin Conformations
Chromosomal DNA Topology: Topoisomerases Handle Extremely Supercoiled, Catenated and Knotted Chromatin Conformations Open
Trabajo presentado en las DNA Topoisomerases in Biology and Medicine Gordon Research Conferences: The Interplay of DNA Topoisomerases and DNA Topology in Biology - Emerging Technologies, Dynamics of Enzyme Function and Opportunities in Dru…
View article: Genome urbanization: Clusters of topologically co-regulated genes delineate functional compartments in the genome of<i>S. cerevisiae</i>
Genome urbanization: Clusters of topologically co-regulated genes delineate functional compartments in the genome of<i>S. cerevisiae</i> Open
The eukaryotic genome evolves under the dual constraint of maintaining co-ordinated gene transcription and performing effective DNA replication and cell division, the coupling of which brings about inevitable DNA topological tension. DNA s…