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Tanycytic annexinA1-containing extracellular vesicles control thermogenesis by orchestrating microglial and neuronal functions Open
SUMMARY Obesity, a major global health issue, results from disrupted energy balance driven by chronic hypothalamic inflammation and altered intercellular communication. Among the diverse cells orchestrating this regulation, tanycytes—speci…
Foam Formation in Shake Flasks and Its Consequences Open
Foam formation in stirred tank fermentation processes is a well‐studied phenomenon. However, foaming in shake flask cultivations is rarely considered. Non‐baffled shake flasks, in particular, are generally considered to prevent foaming pro…
Online monitoring of the mitochondrial respiration activity and protein formation in the Almost Living Cell-free Expression (ALiCE) system Open
Background Cell-free protein synthesis (CFPS) is one approach to address the increasing demand for complex recombinant proteins in various applications, especially in the pharmaceutical sector. CFPS offers a variety of advantages like the …
Application of online spectroscopic techniques for detection of Bacillus spores in cultivations with complex medium Open
Bacillus spores are important in a range of biotechnological applications. Real-time spore detection methods are advantageous for effectively characterizing and optimizing the spore production processes. In this study, a qualitative high-t…
Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures Open
Shake flasks are among the most relevant culture vessels for early-stage process development of viscous microbial cultures. While online process monitoring systems are available for temperature, pH, biomass concentration, dissolved oxygen …
Primary high-throughput screening of engineered phytases by online monitoring of the oxygen transfer rate of Komagataella phaffii Open
Background Recombinant phytase production has recently gained increased recognition in phosphate recycling from phytate contained in plant-based side and waste streams. Until now, new phytase variants are evaluated at the end of the expres…
Small-scale fed-batch cultivations of Vibrio natriegens: overcoming challenges for early process development Open
Vibrio natriegens is a fast-growing microbial workhorse with high potential for biotechnological applications. However, handling the bacterium in batch processes is challenging due to its high overflow metabolism and mixed acid formation u…
Effect of High‐Speed Shaking on Oxygen Transfer in Shake Flasks Open
Shake flasks are predominantly used in screening and the early stages of biotechnological process development. However, oxygen‐demanding processes cannot easily be performed in shake flasks, since the maximum oxygen transfer capacity is us…
Citrate Supplementation Modulates Medium Viscosity and Poly‐γ‐Glutamic Acid Synthesis by Engineered <i>B. subtilis</i> 168 Open
The industrially attractive biopolymer poly‐γ‐glutamic acid (γ‐PGA) is commonly produced by species of the genus Bacillus by co‐feeding different carbon‐ and nitrogen‐sources. Recent studies have highlighted the pivotal role of co‐metaboli…
Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression Open
The transcriptional factors control the expression of many genes and represent an important layer of complexity in cell factories. However, the effect of individual sigma factor deletions from a biomanufacturing perspective has not been ad…
Online monitored characterization of <i>Phocaeicola vulgatus</i> for organic acid production using anaerobic microtiter plate cultivations Open
Phocaeicola vulgatus (formerly Bacteroides vulgatus ), an anaerobic gut bacterium, produces several organic acids. Research on P. vulgatus is still in its infancy. However, a detailed understanding of P. vulgatus growth and metabolism is e…
Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate Open
The transition towards sustainable bioprocesses requires renewable feedstocks to reduce dependency on finite resources. While plant-based feedstocks offer significant potential, their complex composition poses new challenges. The microorga…
High‐sensitivity real‐time monitoring of <span>pH</span> and respiration activity unveils metabolic dynamics in shake flask cultures Open
Erlenmeyer shake flasks are widely used during the first steps of bioprocess development. Despite their broad application in academia and industry, shake flasks usually lack standardized and user‐friendly online monitoring techniques. In t…
Exploration of the Out‐of‐Phase Phenomenon in Shake Flasks by CFD Calculations of Volumetric Power Input, k<sub>L</sub>a Value and Shear Rate at Elevated Viscosity Open
Culture broth with secreted macromolecules and culture broth of filamentous fungi showing disperse growth exhibit elevated viscosity, usually with shear‐thinning flow behavior. High viscosity, however, poses a serious challenge in the prod…
Rheology and Culture Reproducibility of Filamentous Microorganisms: Impact of Flow Behavior and Oxygen Transfer During Salt‐Enhanced Cultivation of the Actinomycete <i>Actinomadura namibiensis</i> Open
Analyzing the relationship between cell morphology, rheological characteristics, and production dynamics of cultivations with filamentous microorganisms is a challenging task. The complex interdependencies and the commonly low reproducibil…
Sigma factors as potential targets to enhance recombinant protein expression Open
The transcriptional factors control the expression of many genes and represent an important layer of complexity in cell factories. However, the effect of individual sigma factor deletions from a biomanufacturing perspective has not been ad…
Impact of hydromechanical stress on CHO cells’ metabolism and productivity: Insights from shake flask cultivations with online monitoring of the respiration activity Open
The hydromechanical stress is a relevant parameter for mammalian cell cultivations, especially regarding scale-up processes. It describes the mechanical forces exerted on cells in a bioreactor. The maximum local energy dissipation rate is …
Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes Open
Background Vibrio natriegens , a halophilic marine γ-proteobacterium, holds immense biotechnological potential due to its remarkably short generation time of under ten minutes. However, the highest growth rates have been primarily observed…
Balancing pH and yield: exploring itaconic acid production in Ustilago cynodontis from an economic perspective Open
Background Itaconic acid is a promising bio-based building block for the synthesis of polymers, plastics, fibers and other materials. In recent years, Ustilago cynodontis has emerged as an additional itaconate producing non-conventional ye…
Phosphate limitation enhances malic acid production on nitrogen-rich molasses with Ustilago trichophora Open
Background An important step in replacing petrochemical products with sustainable, cost-effective alternatives is the use of feedstocks other than, e.g., pure glucose in the fermentative production of platform chemicals. Ustilaginaceae off…
Adaptive laboratory evolution in a novel parallel shaken pH‐auxostat Open
Adaptive laboratory evolution (ALE) is a widely used microbial strain development and optimization method. ALE experiments, to select for faster‐growing strains, are commonly performed as serial batch cultivations in shake flasks, serum bo…
Diffusion-driven fed-batch fermentation in perforated ring flasks Open
Purpose Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional perforated inner glas…
Online monitoring of the respiration activity in 96‐deep‐well microtiter plate Chinese hamster ovary cultures streamlines kill curve experiments Open
Cell line generation of mammalian cells is a time‐consuming and labor‐intensive process, especially because of challenges in clone selection after transfection. Antibiotics are common selection agents for mammalian cells due to their simpl…
Transport‐controlled growth decoupling for self‐induced protein expression with a glycerol‐repressible genetic circuit Open
Decoupling cell formation from recombinant protein synthesis is a potent strategy to intensify bioprocesses. Escherichia coli strains with mutations in the glucose uptake components lack catabolite repression, display low growth rate, no o…