Explanipedia

Structural and functional studies of the EGF20-27 region reveal new features of the human Notch receptor important for optimal activation Open

Zhihan Bo, T.J.W. Rowntree, Steven Johnson, Hilman Nurmahdi, Richard J. Suckling , et al. · 2024

The Notch receptor is activated by the Delta/Serrate/Lag-2 (DSL) family of ligands. The organization of the extracellular signaling complex is unknown, although structures of Notch/ligand complexes comprising the ligand-binding region (LBR…

Rescue of secretion of rare‐disease‐associated misfolded mutant glycoproteins in UGGT1 knock‐out mammalian cells Open

Gábor Tax, Kevin P. Guay, Ludovica Pantalone, Martina Ceci, Tatiana Soldà , et al. · 2024

Endoplasmic reticulum (ER) retention of misfolded glycoproteins is mediated by the ER‐localized eukaryotic glycoprotein secretion checkpoint, UDP‐glucose glycoprotein glucosyl‐transferase (UGGT). The enzyme recognizes a misfolded glycoprot…

A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpoint Open

Kevin P. Guay, Roberta Ibba, J. L. Kiappes, Snežana Vasiljević, Francesco Bonì , et al. · 2023

Misfolded glycoprotein recognition and endoplasmic reticulum (ER) retention are mediated by the ER glycoprotein folding quality control (ERQC) checkpoint enzyme, UDP-glucose glycoprotein glucosyltransferase (UGGT). UGGT modulation is a pro…

Rescue of secretion of a rare-disease associated mis-folded mutant glycoprotein inUGGT1knock-out mammalian cells Open

Gábor Tax, Kevin P. Guay, Tatiana Soldà, Charlie J. Hitchman, Johan C. Hill , et al. · 2023

Endoplasmic reticulum (ER) retention of mis-folded glycoproteins is mediated by the ER- localised eukaryotic glycoprotein secretion checkpoint, UDP-glucose glycoprotein glucosyl-transferase (UGGT). The enzyme recognises a mis-folded glycop…

A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpoint Open

Kevin P. Guay, Roberta Ibba, J.L. Kiappes, Maria De Benedictis, Ilaria Zeni , et al. · 2022

The Endoplasmic Reticulum (ER) glycoprotein folding Quality Control (ERQC) machinery aids folding of glycoproteins in the ER. Misfolded glycoprotein recognition and ER-retention is mediated by the ERQC checkpoint enzyme, the 170 kDa UDP-Gl…

A Quinolin-8-Ol Sub-Millimolar Inhibitor of UGGT, the ER Glycoprotein Folding Quality Control Checkpoint Open

Kevin P. Guay, Roberta Ibba, J.L. Kiappes, Maria De Benedictis, Ilaria Zeni , et al. · 2022

Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase Open

Carlos P. Modenutti, Juan I. Blanco Capurro, Roberta Ibba, Dominic S. Alonzi, Mauro N. Song , et al. · 2020

UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention. We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain…

Development of autotaxin inhibitors: A series of tetrazole cinnamides Open

Christopher G. Thomson, Darren Le Grand, Mark R. Dowling, David T. Beattie, Lucy M. Elphick , et al. · 2020

Clamping, Bending, and Twisting Inter-Domain Motions in the Misfold-Recognising Portion of UDP-Glucose: Glycoprotein Glucosyl-Transferase Open

Carlos P. Modenutti, Juan I. Blanco Capurro, Roberta Ibba, Dominic S. Alonzi, Snežana Vasiljević , et al. · 2020

Clamping, bending, and twisting inter-domain motions in the misfold-recognising portion of UDP-glucose:glycoprotein glucosyl-transferase Open

Carlos P. Modenutti, Juan I. Blanco Capurro, Roberta Ibba, Snežana Vasiljević, Mario Hensen , et al. · 2019

Summary UDP-glucose:glycoprotein glucosyltransferase (UGGT) is the only known glycoprotein folding quality control checkpoint in the eukaryotic glycoprotein secretory pathway. When the enzyme detects a misfolded glycoprotein in the Endopla…

Crystal Structures Determination Of Ctuggt-N Aka Ctuggt S180C:T742C Open

Pietro Roversi, Roberta Ibba, Mario Hensen, Johan C. Hill · 2018

Two crystal structures of the double Cys mutant CtUGGT-N aka CtUGGT S180C:T742C have been determined by X-ray crystallography. The trigonal one, in space group P3₂12 was determined at 4.7 Å resolution …

Crystal Structures Determination Of Ctuggt-N Aka Ctuggt S180C:T742C Open

Pietro Roversi, Roberta Ibba, Mario Hensen, Johan C. Hill · 2018

Two crystal structures of the double Cys mutant CtUGGT-N aka CtUGGT S180C:T742C have been determined by X-ray crystallography. The trigonal one, in space group P3₂12 was determined at 4.7 Å resolution …

Development of autotaxin inhibitors: A series of zinc binding triazoles Open

Christopher G. Thomson, Darren Le Grand, Mark R. Dowling, Cara E. Brocklehurst, Colin Chinn , et al. · 2018

Structural characterisation of calnexin cycle components and assessment as antiviral targets Open

Johan C. Hill · 2018

N-glycosylated proteins that traverse the endoplasmic reticulum (ER) can make use of the calnexin cycle to attain their correct fold. The calnexin cycle modifies the N-glycan structure and allows for association of the glycoprotein with th…

Target highlights from the first post‐PSI CASP experiment (CASP12, May–August 2016) Open

Andriy Kryshtafovych, Reinhard Albrecht, Arnaud Baslé, Pedro Bule, Alessandro T. Caputo , et al. · 2017

The functional and biological significance of the selected CASP12 targets are described by the authors of the structures. The crystallographers discuss the most interesting structural features of the target proteins and assess whether thes…

Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint Open

Pietro Roversi, Lucia Marti, Alessandro T. Caputo, Dominic S. Alonzi, Johan C. Hill , et al. · 2017

Significance A dedicated endoplasmic reticulum quality control (ERQC) machinery ensures the correct fold of secreted proteins bearing N-linked glycans, which constitute around a fifth of the whole proteome and are essential for many import…

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