Explanipedia

High-throughput Single-Cell Proteomics and Transcriptomics from the Same Cells with a Nanoliter-Scale Spin-Transfer Approach Open

Pranav Dawar, Lye Meng Markillie, Sarah Williams, Hugh Mitchell, Johannes Bagnoli , et al. · 2025

Single-cell multiomic platforms provide a comprehensive snapshot of cellular states and cell types by offering critical insights into the spatiotemporal regulation of biomolecular networks at a systems level, thereby defining the basis of …

Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting Open

James Fulcher, Lye Meng Markillie, Hugh Mitchell, Sarah Williams, Kristin Engbrecht , et al. · 2024

Single-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), a…

CSF3R T618I Collaborates With RUNX1-RUNX1T1 to Expand Hematopoietic Progenitors and Sensitizes to GLI Inhibition Open

Anja Swoboda, Vanessa Cristina Arfelli, Anna Danese, Roland Windisch, Paul Kerbs , et al. · 2023

Activating colony-stimulating factor-3 receptor gene ( CSF3R ) mutations are recurrent in acute myeloid leukemia (AML) with t(8;21) translocation. However, the nature of oncogenic collaboration between alterations of CSF3R and the t(8;21) …

Wound infiltrating adipocytes are not myofibroblasts Open

Shruthi Kalgudde Gopal, Ruoxuan Dai, Ania Stefańska, Meshal Ansari, Jiakuan Zhao , et al. · 2023

UHMK1 is a novel splicing regulatory kinase Open

Vanessa Cristina Arfelli, Yun-Chien Chang, Johannes Bagnoli, Paul Kerbs, Felipe E. Ciamponi , et al. · 2023

Data for EMSL Project 51834 from January 2023 Open

Lye Meng Markillie, Ying Zhu, Carter Bracken, Tony Chiang, Joshua Cantlon-Bruce , et al. · 2023

Data for EMSL Project 51834 from January 2023 Open

Lye Meng Markillie, Ying Zhu, Carter Bracken, Tony Chiang, Joshua Cantlon-Bruce , et al. · 2023

Data for EMSL Project 51834 from January 2023 Open

Lye Meng Markillie, Ying Zhu, Carter Bracken, Tony Chiang, Joshua Cantlon-Bruce , et al. · 2023

Ly6D+Siglec-H+ precursors contribute to conventional dendritic cells via a Zbtb46+Ly6D+ intermediary stage Open

Konstantin Lutz, Andrea Musumeci, Christopher Sie, Ezgi Dursun, Elena Winheim , et al. · 2022

UHMK1 is a novel splicing regulatory kinase Open

Vanessa Cristina Arfelli, Yun-Chien Chang, Johannes Bagnoli, Paul Kerbs, Felipe E. Ciamponi , et al. · 2022

The U2AF Homology Motif Kinase 1 (UHMK1) is the only kinase that contains the U2AF homology motif (UHM), a common protein interaction domain among splicing factors. Through this motif, UHMK1 interacts with the splicing factors SF1 and SF3B…

Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting Open

James Fulcher, Lye Meng Markillie, Hugh Mitchell, Sarah Williams, Kristin Engbrecht , et al. · 2022

Single-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), a…

prime-seq v2 Open

Aleksandar Janjic, Lucas E. Wange, Johannes Bagnoli, Johanna Geuder, Phong Nguyen , et al. · 2022

Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruS…

prime-seq v2 Open

Aleksandar Janjic, Lucas E. Wange, Johannes Bagnoli, Johanna Geuder, Phong Thanh Nguyen , et al. · 2022

Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruS…

Prime-seq, efficient and powerful bulk RNA sequencing Open

Aleksandar Janjic, Lucas E. Wange, Johannes Bagnoli, Johanna Geuder, Phong Nguyen , et al. · 2022

Adverse stem cell clones within a single patient’s tumor predict clinical outcome in AML patients Open

Christina Zeller, Daniel Richter, Vindi Jurinović, Ilse A. Valtierra-Gutiérrez, Ashok Kumar Jayavelu , et al. · 2022

Data for EMSL Project 51834 from October 2022 Open