John A. Cooper
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View article: Initial treatment approaches for nodular gastric antral vascular ectasia: A comparison of endoscopic band ligation and thermal therapies
Initial treatment approaches for nodular gastric antral vascular ectasia: A comparison of endoscopic band ligation and thermal therapies Open
BACKGROUND Gastric antral vascular ectasia (GAVE) accounts for up to 4% of nonvariceal upper gastrointestinal bleeding. Argon plasma coagulation and radiofrequency ablation have been primary treatment modalities for patients with linear an…
Coordinated regulation of Capping Protein by CARMIL and V-1/myotrophin Open
Capping Protein (CP) plays a central role in controlling actin filament dynamics in almost all eukaryotic cell types by virtue of its ability to block monomer addition and loss at the barbed end of the actin filament. Like other proteins t…
Unveiling the Complexity in the Management and Outcomes of Traumatic Versus Non-Traumatic Bile Leaks—A Comparative Analysis Open
Background and Aims Endoscopic retrograde cholangiopancreatography (ERCP) is a crucial diagnostic and therapeutic modality for managing bile leaks, particularly in the context of traumatic etiologies. Here, we aimed to contribute to this b…
Correction: Dynein and Dynactin Leverage Their Bivalent Character to Form a High-Affinity Interaction Open
[This corrects the article DOI: 10.1371/journal.pone.0059453.].
Reconstitution of Arp2/3-Nucleated Actin Assembly with CP, V-1 and CARMIL Open
Summary Actin polymerization is often associated with membrane proteins containing capping-protein-interacting (CPI) motifs, such as CARMIL, CD2AP, and WASHCAP/Fam21. CPI motifs bind directly to actin capping protein (CP), and this interac…
Capping protein regulators of actin assembly in budding yeast Open
Cellular functions of actin capping protein (CP) regulators are poorly understood. Di Pietro and colleagues (https://doi.org/10.1083/jcb.202306154) shed unprecedented light on this topic using budding yeast. Two proteins with CPI (capping …
View article: Biophysical Mechanism of Allosteric Regulation of Actin Capping Protein
Biophysical Mechanism of Allosteric Regulation of Actin Capping Protein Open
Actin capping protein (CP) can be regulated by steric and allosteric mechanisms. The molecular mechanism of the allosteric regulation at a biophysical level includes linkage between the binding sites for three ligands: F-actin, Capping-Pro…
Septin and actin contributions to endothelial cell–cell junctions and monolayer integrity Open
Septins in endothelial cells (ECs) have important roles supporting the integrity of the endothelial monolayer. Cell–cell junctions in EC monolayers are highly dynamic, with continuous retractions and protrusions. Depletion of septins in EC…
Actin capping protein regulates postsynaptic spine development through CPI-motif interactions Open
Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable…
A randomised trial of a web-based physical activity self-management intervention in COPD Open
Improving exercise capacity is a primary objective in COPD. Declines in exercise capacity result in reduced physical activity and health-related quality of life (HRQoL). Self-management interventions can teach patients skills and behaviour…
Targeting primary and metastatic uveal melanoma with a G protein inhibitor Open
Uveal melanoma (UM) is the most common intraocular tumor in adults. Nearly half of UM patients develop metastatic disease and often succumb within months because effective therapy is lacking. A novel therapeutic approach has been suggested…
Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium Open
Uveal melanoma is highly metastatic and only disseminates hematogenously, so that transendothelial migration is vital to metastasis. Uveal melanoma cells switch from amoeboid to mesenchymal motility, controlled by the Rho and Rac pathways,…
Junctional Localization of Septin 2 Is Required for Organization of Junctional Proteins in Static Endothelial Monolayers Open
Objective: Septin 2 is localized at junctions in human microvascular endothelial monolayers. The junctional localization of septin 2 is necessary for organization of cell-cell adhesion proteins of endothelial cells. Approach and Results: S…
CARMIL3 is important for cell migration and morphogenesis during early development in zebrafish Open
Cell migration is important during early animal embryogenesis. Cell migration and cell shape are controlled by actin assembly and dynamics, which depend on capping proteins, including the barbed-end heterodimeric actin capping protein (CP)…
Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium Open
SUMMARY Uveal melanomas (UM) are malignant cancers arising from the pigmented layers of the eye. UM cells spread through the bloodstream, and circulating UM cells are detectable in patients before metastases appear. Extravasation of UM cel…
Comparative Analysis of CPI-Motif Regulation of Biochemical Functions of Actin Capping Protein Open
The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding se…
Septin Roles and Mechanisms in Organization of Endothelial Cell Junctions Open
Septins play an important role in regulating the barrier function of the endothelial monolayer of the microvasculature. Depletion of septin 2 protein alters the organization of vascular endothelial (VE)-cadherin at cell-cell adherens junct…
Comparative analysis of CPI-motif regulation of biochemical functions of actin capping protein Open
The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding se…
Metoprolol for the Prevention of Acute Exacerbations of COPD Open
Among patients with moderate or severe COPD who did not have an established indication for beta-blocker use, the time until the first COPD exacerbation was similar in the metoprolol group and the placebo group. Hospitalization for exacerba…