John A. G. Briggs
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View article: The mechanistic basis of cargo selection during Golgi maturation
The mechanistic basis of cargo selection during Golgi maturation Open
The multiple cisternae of the Golgi apparatus contain resident membrane proteins crucial for lipid and protein glycosylation. How Golgi residents remain in their designated compartments despite a constant flow of secretory cargo is incompl…
View article: The molecular infrastructure of glutamatergic synapses in the mammalian forebrain
The molecular infrastructure of glutamatergic synapses in the mammalian forebrain Open
Glutamatergic synapses form the vast majority of connections within neuronal circuits, but how these subcellular structures are molecularly organized within the mammalian brain is poorly understood. Conventional electron microscopy using c…
View article: The molecular infrastructure of glutamatergic synapses in the mammalian forebrain
The molecular infrastructure of glutamatergic synapses in the mammalian forebrain Open
Glutamatergic synapses form the vast majority of connections within neuronal circuits, but how these subcellular structures are molecularly organized within the mammalian brain is poorly understood. Conventional electron microscopy using c…
View article: The mechanistic basis of cargo selection during Golgi maturation
The mechanistic basis of cargo selection during Golgi maturation Open
The multiple cisternae of the Golgi apparatus contain resident membrane proteins crucial for lipid and protein glycosylation. How Golgi residents remain in their designated compartments despite a constant flow of secretory cargo is incompl…
View article: In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-ET
In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-ET Open
Long terminal repeat (LTR) retrotransposons belong to the transposable elements (TEs), autonomously replicating genetic elements that integrate into the host's genome. Among animals, Drosophila melanogaster serves as an important model org…
View article: The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation
The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation Open
The virus particles of human immunodeficiency virus type 1 (HIV-1) are released in an immature, non-infectious form. Proteolytic cleavage of the main structural polyprotein Gag into functional domains induces rearrangement into mature, inf…
View article: Anti-Ebola virus mAb 3A6 protects highly viremic animals from fatal outcome via binding GP(1,2) in a position elevated from the virion membrane
Anti-Ebola virus mAb 3A6 protects highly viremic animals from fatal outcome via binding GP(1,2) in a position elevated from the virion membrane Open
Monoclonal antibodies (mAbs) against Ebola virus (EBOV) glycoprotein (GP 1,2 ) are the standard of care for Ebola virus disease (EVD). Anti-GP 1,2 mAbs targeting the stalk and membrane proximal external region (MPER) potently neutralize EB…
View article: Towards community-driven visual proteomics with large-scale cryo-electron tomography of <i>Chlamydomonas reinhardtii</i>
Towards community-driven visual proteomics with large-scale cryo-electron tomography of <i>Chlamydomonas reinhardtii</i> Open
In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production of large-scale cryo-ET datasets, as we…
View article: A physical model for M1-mediated influenza A virus assembly
A physical model for M1-mediated influenza A virus assembly Open
Influenza A virus particles assemble at the plasma membrane of infected cells. During assembly all components of the virus come together in a coordinated manner to deform the membrane into a protrusion eventually forming a new, membrane-en…
View article: Virion morphology and on-virus spike protein structures of diverse SARS-CoV-2 variants
Virion morphology and on-virus spike protein structures of diverse SARS-CoV-2 variants Open
The evolution of SARS-CoV-2 variants with increased fitness has been accompanied by structural changes in the spike (S) proteins, which are the major target for the adaptive immune response. Single-particle cryo-EM analysis of soluble S pr…
View article: The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation
The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation Open
HIV-1 particles are released in an immature, non-infectious form. Proteolytic cleavage of the main structural polyprotein Gag into functional domains induces rearrangement into mature, infectious virions. In immature virus particles, the G…
View article: A physical model for M1-mediated influenza A virus assembly
A physical model for M1-mediated influenza A virus assembly Open
Influenza A virus particles assemble at the plasma membrane of infected cells. During assembly all components of the virus come together in a coordinated manner to deform the membrane into a protrusion eventually forming a new, membrane-en…
View article: Ultrastructural Dynamics of Dopaminergic Presynaptic Release Sites revealed by Cryo-correlative Light and Electron Microscopy
Ultrastructural Dynamics of Dopaminergic Presynaptic Release Sites revealed by Cryo-correlative Light and Electron Microscopy Open
Dopaminergic neurons are fundamental in governing motivation, movement, and many aspects of cognition. The targeted modulation of dopaminergic signaling serves as a cornerstone in developing therapeutic interventions for conditions such as…
View article: In-cell structure and snapshots of<i>copia</i>retrotransposons in intact tissue by cryo-electron tomography
In-cell structure and snapshots of<i>copia</i>retrotransposons in intact tissue by cryo-electron tomography Open
Long terminal repeat (LTR) retrotransposons belong to the transposable elements (TE), autonomously replicating genetic elements that integrate into the host’s genome. LTR retrotransposons represent a major component of genomes across the t…
View article: Virion morphology and on-virus spike protein structures of diverse SARS-CoV-2 variants
Virion morphology and on-virus spike protein structures of diverse SARS-CoV-2 variants Open
The evolution of SARS-CoV-2 variants with increased fitness has been accompanied by structural changes in the spike (S) proteins that are the major target for the adaptive immune response. Single-particle cryo-EM analysis of soluble S from…
View article: Zieria nubicola (Rutaceae), a highly restricted and newly described species from New South Wales, Australia
Zieria nubicola (Rutaceae), a highly restricted and newly described species from New South Wales, Australia Open
Zieria nubicola H.K.Orel & Duretto sp. nov. (Rutaceae), a highly restricted species from Cambewarra Mountain on the south-central coast of New South Wales, is formally described and illustrated. Notes on the species distribution, habitat a…
View article: Molecular dynamics simulations of HIV-1 matrix-membrane interactions at different stages of viral maturation
Molecular dynamics simulations of HIV-1 matrix-membrane interactions at different stages of viral maturation Open
Although the structural rearrangement of the membrane-bound matrix (MA) protein trimers upon HIV-1 maturation has been reported, the consequences of MA maturation on the MA-lipid interactions are not well understood. Long-timescale molecul…
View article: Towards the Visual Proteomics of <i>C. reinhardtii</i> using High-throughput Collaborative <i>in situ</i> Cryo-ET
Towards the Visual Proteomics of <i>C. reinhardtii</i> using High-throughput Collaborative <i>in situ</i> Cryo-ET Open
Journal Article Towards the Visual Proteomics of C. reinhardtii using High-throughput Collaborative in situ Cryo-ET Get access Sagar Khavnekar, Sagar Khavnekar Cryo-EM Technology group, Max Planck Institute of Biochemistry, Martinsried, Ge…