John P. Cooke
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View article: APOA1 binding protein promotes lymphatic cell fate and lymphangiogenesis by relieving caveolae-mediated inhibition of VEGFR3 signaling
APOA1 binding protein promotes lymphatic cell fate and lymphangiogenesis by relieving caveolae-mediated inhibition of VEGFR3 signaling Open
The lymphatic system maintains tissue fluid balance, and its dysfunction can result in lymphedema. Although cholesterol is essential for cellular function, its role in lymphatic development has remained unknown. Here, we identify APOA1 bin…
View article: Telomerase mRNA therapy protects human skin against radiation-induced DNA damage
Telomerase mRNA therapy protects human skin against radiation-induced DNA damage Open
Radiation therapy, while effective against cancer, often causes collateral damage to surrounding healthy tissues, leading to DNA damage that can precipitate genomic instability and cancer. Despite the enormity of the problem, there is curr…
View article: Transcription factor 4 maintains endothelial cell identity by inhibiting endothelial to mesenchymal transition
Transcription factor 4 maintains endothelial cell identity by inhibiting endothelial to mesenchymal transition Open
Endothelial to mesenchymal transition (EndoMT) is essential for embryonic heart development and contributes to many pathological processes. It is unclear how the balance between endothelial cell (EC) identity and EndoMT mediators is regula…
View article: Downregulation of LATS1/2 Drives Endothelial Senescence-Associated Stemness (SAS) and Atherothrombotic Lesion Formation
Downregulation of LATS1/2 Drives Endothelial Senescence-Associated Stemness (SAS) and Atherothrombotic Lesion Formation Open
Background Atherothrombosis, the main event leading to acute coronary syndrome (ACS), is strongly linked to disturbed blood flow (d-flow) regions. Although the involvement of the Hippo pathway and its kinases Large Tumor Suppressor Kinase …
View article: A visual–omics foundation model to bridge histopathology with spatial transcriptomics
A visual–omics foundation model to bridge histopathology with spatial transcriptomics Open
Artificial intelligence has revolutionized computational biology. Recent developments in omics technologies, including single-cell RNA sequencing and spatial transcriptomics, provide detailed genomic data alongside tissue histology. Howeve…
View article: A visual–omics foundation model to bridge histopathology image with transcriptomics
A visual–omics foundation model to bridge histopathology image with transcriptomics Open
Artificial intelligence has revolutionized computational biology. Recent developments in omics technologies, including single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST), provide detailed genomic data alongside tissue …
View article: Gallium disrupts endoplasmic reticulum iron metabolism to induce lipid metabolic reprogramming in glioblastoma cells
Gallium disrupts endoplasmic reticulum iron metabolism to induce lipid metabolic reprogramming in glioblastoma cells Open
Gallium based therapeutic strategies are thought to be an effective means to promote tumor cell killing that are currently being investigated in glioblastoma. Gallium is a group IIIa metal that is redox inactive, however, its atomic simila…
View article: The impact of T7 RNA polymerase class II pause sites on mRNA therapeutics
The impact of T7 RNA polymerase class II pause sites on mRNA therapeutics Open
Background and purpose: There is growing interest in messenger ribonucleic acid (mRNA) drugs for a wide variety of applications including cardiovascular disease. Typically, mRNA drug substances are generated from linear deoxyribonucleic ac…
View article: Modulation of cell fate by shock wave therapy in ischaemic heart disease
Modulation of cell fate by shock wave therapy in ischaemic heart disease Open
Aims Cardiac shockwave therapy (SWT) improves left ventricular (LV) function in patients with ischaemic cardiomyopathy. Shockwave therapy activates Toll-like receptor 3 (TLR3), a receptor-inducing chromatin remodelling and nuclear reprogra…
View article: Circular <scp>RNA</scp> Telomerase Reverses Endothelial Senescence in Progeria
Circular <span>RNA</span> Telomerase Reverses Endothelial Senescence in Progeria Open
Telomeres shorten with each cell division, acting as a chronometer of cell age. The enzyme telomerase, primarily active in stem cells, reverses telomere erosion. We have previously observed that transient transfection with human TERT mRNA …
View article: Telomerase mRNA Reduces Radiation-induced DNA Damage of human skin
Telomerase mRNA Reduces Radiation-induced DNA Damage of human skin Open
Over four million people undergo radiation therapy annually in the United States. Among these, more than 90% experience varying degrees of radiation-induced skin injury. Despite the enormity of the problem, there is currently no FDA-approv…
View article: Recovery from Heart Failure is a Vascular Recovery
Recovery from Heart Failure is a Vascular Recovery Open
Heart failure (HF) remains a major cause of morbidity and mortality worldwide, with limited treatment options. Heart transplantation is an end stage option but limited by donor availability. Left-ventricular assist device (LVAD) implantati…
View article: Cardiac shockwave therapy in addition to coronary bypass surgery improves myocardial function in ischaemic heart failure: the CAST-HF trial
Cardiac shockwave therapy in addition to coronary bypass surgery improves myocardial function in ischaemic heart failure: the CAST-HF trial Open
Background and Aims In chronic ischaemic heart failure, revascularisation strategies control symptoms but are less effective in improving left ventricular ejection fraction (LVEF). The aim of this trial is to investigate the safety of card…
View article: β-catenin mRNA encapsulated in SM-102 lipid nanoparticles enhances bone formation in a murine tibia fracture repair model
β-catenin mRNA encapsulated in SM-102 lipid nanoparticles enhances bone formation in a murine tibia fracture repair model Open
Fractures continue to be a global economic burden as there are currently no osteoanabolic drugs approved to accelerate fracture healing. In this study, we aimed to develop an osteoanabolic therapy which activates the Wnt/β-catenin pathway,…
View article: Mapping Endothelial-Macrophage Interactions in Diabetic Vasculature: Role of TREM2 in Vascular Inflammation and Ischemic Response
Mapping Endothelial-Macrophage Interactions in Diabetic Vasculature: Role of TREM2 in Vascular Inflammation and Ischemic Response Open
Diabetes mellitus (DM) significantly accelerates vascular diseases like peripheral arterial disease (PAD). Endothelial cells (ECs) and macrophages (MΦs) singularly and synergistically are important contributors to DM-associated vascular dy…
View article: TNIK regulation of interferon signaling and endothelial cell response to virus infection
TNIK regulation of interferon signaling and endothelial cell response to virus infection Open
Background Traf2 and Nck-interacting kinase (TNIK) is known for its regulatory role in various processes within cancer cells. However, its role within endothelial cells (ECs) has remained relatively unexplored. Methods Leveraging RNA-seq d…
View article: Telomerase mRNA Enhances Human Skin Engraftment for Wound Healing (Adv. Healthcare Mater. 2/2024)
Telomerase mRNA Enhances Human Skin Engraftment for Wound Healing (Adv. Healthcare Mater. 2/2024) Open
Lipid Nanoparticles In article 2302029 by John P. Cooke, Biana Godin, and co-workers, lipid nanoparticles carrying human telomerase reverse transcriptase mRNA (hTERT-mRNA LNP) are formulated for transfection in human skin cells suspension …
View article: Myocardial Recovery versus Myocardial Regeneration: Mechanisms and Therapeutic Modulation
Myocardial Recovery versus Myocardial Regeneration: Mechanisms and Therapeutic Modulation Open
Myocardial recovery is characterized by a return toward normal structure and function of the heart after an injury. Mechanisms of myocardial recovery include restoration and/or adaptation of myocyte structure and function, mitochondrial ac…
View article: Sociodemographic and clinical correlates of physical therapy utilization in adults with symptomatic knee osteoarthritis
Sociodemographic and clinical correlates of physical therapy utilization in adults with symptomatic knee osteoarthritis Open
Background. Physical therapy is essential for conservative management of symptomatic knee osteoarthritis (OA). However, physical therapy utilization data are limited for knee OA. Objective. The purpose of this study is to identify correlat…
View article: Racial Differences in Performance-Based Function and Potential Explanatory Factors Among Individuals With Knee Osteoarthritis
Racial Differences in Performance-Based Function and Potential Explanatory Factors Among Individuals With Knee Osteoarthritis Open
Objective: In individuals with knee osteoarthritis (OA), self-reported physical function is poorer in African Americans than in whites, but whether this difference holds true for objective assessments is unclear. The purpose of this study …
View article: Premature senescence and cardiovascular disease following cancer treatments: mechanistic insights
Premature senescence and cardiovascular disease following cancer treatments: mechanistic insights Open
Cardiovascular disease (CVD) is a leading cause of morbidity and mortality, especially among the aging population. The “response-to-injury” model proposed by Dr. Russell Ross in 1999 emphasizes inflammation as a critical factor in atherosc…