John E. Chandler
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View article: Correction: Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy
Correction: Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy Open
Correction for ‘Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy’ by Graham L. C. Spicer et al. , Nanoscale , 2018, 10 , 19125–19130, https://doi.org/10.1039/C8NR07481J.
View article: Nanoscale chromatin imaging and analysis platform bridges 4D chromatin organization with molecular function
Nanoscale chromatin imaging and analysis platform bridges 4D chromatin organization with molecular function Open
nano-ChIA combines multiple nanoimaging modalities to uncover 4D chromatin organization and its relationship to molecular function.
View article: Nanoscale Chromatin Imaging and Analysis (nano-ChIA) platform bridges 4-D chromatin organization with molecular function
Nanoscale Chromatin Imaging and Analysis (nano-ChIA) platform bridges 4-D chromatin organization with molecular function Open
In eukaryotic cells, chromatin structure is linked to transcription processes through the regulation of genome organization. Extending across multiple length-scales - from the nucleosome to higher-order three-dimensional structures - chrom…
View article: Sub-10-nm imaging of nucleic acids using spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON)
Sub-10-nm imaging of nucleic acids using spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON) Open
Elucidating chromatin structure in vitro requires resolution below 10 nm to visualize the mononucleosome has been an ongoing challenge. In this work, we achieve sub-10-nm imaging of nucleic acids via spectroscopic intrinsic-contrast photon…
View article: Multimodal interferometric imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm
Multimodal interferometric imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm Open
We present a multimodal label-free interferometric imaging platform for measuring intracellular nanoscale structure and macromolecular dynamics in living cells with a sensitivity to macromolecules as small as 20nm and millisecond temporal …
View article: The Transformation of the Nuclear Nanoarchitecture in Human Field Carcinogenesis
The Transformation of the Nuclear Nanoarchitecture in Human Field Carcinogenesis Open
Morphological alterations of the nuclear texture are a hallmark of carcinogenesis. At later stages of disease, these changes are well characterized and detectable by light microscopy. Evidence suggests that similar albeit nanoscopic altera…
View article: Stochastic fluorescence switching of nucleic acids under visible light illumination
Stochastic fluorescence switching of nucleic acids under visible light illumination Open
We report detailed characterizations of stochastic fluorescence switching of unmodified nucleic acids under visible light illumination. Although the fluorescent emission from nucleic acids under the visible light illumination has long been…
View article: The Global Relationship between Chromatin Physical Topology, Fractal Structure, and Gene Expression
The Global Relationship between Chromatin Physical Topology, Fractal Structure, and Gene Expression Open
Most of what we know about gene transcription comes from the view of cells as molecular machines: focusing on the role of molecular modifications to the proteins carrying out transcriptional reactions at a loci-by-loci basis. This view ign…
View article: Label-free imaging of the native, living cellular nanoarchitecture using partial-wave spectroscopic microscopy
Label-free imaging of the native, living cellular nanoarchitecture using partial-wave spectroscopic microscopy Open
Significance Chromatin is one of the most critical structures within the cell because it houses most genetic information. Its structure is well understood at the nucleosomal (<20-nm) and chromosomal (>200-nm) levels; however, due to the la…
View article: The Greater Genomic Landscape: The Heterogeneous Evolution of Cancer
The Greater Genomic Landscape: The Heterogeneous Evolution of Cancer Open
Results have historically shown a broad plasticity in the origin of tumors and their functions, with significant heterogeneity observed in both morphologies and functional capabilities. Largely unknown, however, are the mechanisms by which…
View article: Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast
Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast Open
Significance Fluorescence photoswitching of native, unmodified deoxyribonucleic acid (DNA) using visible light facilitates the label-free nanoscale imaging of chromatin structures based on the principle of single-molecule photon localizati…
View article: Live Cell Partial Wave Spectroscopic microscopy: Label-free Imaging of the Native, Living Cellular Nano-architecture
Live Cell Partial Wave Spectroscopic microscopy: Label-free Imaging of the Native, Living Cellular Nano-architecture Open
The organization of chromatin is a regulator of molecular processes including transcription, replication, and DNA repair. The structures within chromatin that regulate these processes span from the nucleosomal (10nm) to the chromosomal (>2…
View article: Finite-difference time-domain-based optical microscopy simulation of dispersive media facilitates the development of optical imaging techniques
Finite-difference time-domain-based optical microscopy simulation of dispersive media facilitates the development of optical imaging techniques Open
Combining finite-difference time-domain (FDTD) methods and modeling of optical microscopy modalities, we previously developed an open-source software package called Angora, which is essentially a “microscope in a computer.” However, the sa…
View article: Consensus thermotolerance ranking for 110 <i>Symbiodinium</i> phylotypes: an exemplar utilization of a novel iterative partial‐rank aggregation tool with broad application potential
Consensus thermotolerance ranking for 110 <i>Symbiodinium</i> phylotypes: an exemplar utilization of a novel iterative partial‐rank aggregation tool with broad application potential Open
Summary Corals may reduce the effects of heat‐induced bleaching through associations with thermotolerant algal symbionts ( Symbiodinium) . Although hundreds of Symbiodinium genetic types (phylotypes) are known, thermotolerance has been sys…
View article: Nanoscale refractive index fluctuations detected via sparse spectral microscopy
Nanoscale refractive index fluctuations detected via sparse spectral microscopy Open
Partial Wave Spectroscopic (PWS) Microscopy has proven effective at detecting nanoscale hallmarks of carcinogenesis in histologically normal-appearing cells. The current method of data analysis requires acquisition of a three-dimensional d…