John E. Heuser
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View article: <i>Bordetella pertussis</i> exhibits genomic diversity within patients and laboratory culture
<i>Bordetella pertussis</i> exhibits genomic diversity within patients and laboratory culture Open
Over the past decade, genomic characterization of Bordetella pertussis isolates recovered from US pertussis patients has unveiled noteworthy structural gene order variations. Whole-genome sequencing (WGS) shows that although B. pertussis e…
View article: Defining the EM-signature of successful cell-transfection
Defining the EM-signature of successful cell-transfection Open
In this report, we describe the architecture of Lipofectamine 2000 and 3000 transfection- reagents, as they appear inside of transfected cells, using classical transmission electron microscopy (EM). We also demonstrate that they provoke co…
View article: The AP-2 Adaptor 2 Appendage Scaffolds Alternate Cargo Endocytosis
The AP-2 Adaptor 2 Appendage Scaffolds Alternate Cargo Endocytosis Open
The independently folded appendages of the large α and β2 subunits of the endocytic adaptor protein (AP)-2 complex coordinate proper assembly and operation of endocytic components during clathrin-mediated endocytosis. The β2 subunit append…
View article: Myelination of peripheral nerves is controlled by PI4KB through regulation of Schwann cell Golgi function
Myelination of peripheral nerves is controlled by PI4KB through regulation of Schwann cell Golgi function Open
Significance We identify PI4KB, a phosphoinositide kinase that controls the functions of the Golgi compartment, as a critical regulator of myelination of peripheral nerves. Genetic inactivation of PI4KB, specifically in Schwann cells in mi…
View article: Contacting domains that segregate lipid from solute transporters in malaria parasites
Contacting domains that segregate lipid from solute transporters in malaria parasites Open
While membrane contact sites (MCS) between intracellular organelles are abundant 1 , and cell-cell junctions are classically defined 2 , very little is known about the contacts between membranes that delimit extracellular junctions within …
View article: Rounding precedes rupture and breakdown of vacuolar membranes minutes before malaria parasite egress from erythrocytes
Rounding precedes rupture and breakdown of vacuolar membranes minutes before malaria parasite egress from erythrocytes Open
Because Plasmodium falciparum replicates inside of a parasitophorous vacuole (PV) within a human erythrocyte, parasite egress requires the rupture of two limiting membranes. Parasite Ca2+ , kinases, and proteases contribute to efficient eg…